摘要
Topreparecalcium-bindingpeptides,thefleshresidueofMactraVeneriformiswassubjectedtoenzymatichydrolysis.Bycomparingthecapabilityofcombiningcalciumofthehydrolyzates,pepsinwasconfirmedtobethemostsuitableenzymeforhydrolyzingthefleshresiduetoreleasecalcium-bindingpeptidesamongtheseventestedproteases.Thepepsinhydrolyzate(PHM)wasdividedintothreefractionsaccordingtothemoleculeweightofitscomposition,whichrangedfrom0.5to15kDa.Thelow-molecule-weightfractionnamedPHM-3hadthehighestcapabilityincombiningcalcium.ThepeptidesexistinginthePHM-3fractionconsistedofhighercontentsofGlu,AlaandLeu,andcouldproduceonetypeofcalcium-peptidecomplexbypowerfullychelatingcalciumions.PHM-3productscouldeffectivelyincreasecalciumabsorptionandretentionwhiletheydecreasedthecalciumexcretioninanimaltests.Additionally,symptomscausedbylowcalciumbioavailabilityinovariectomizedrats,suchasbonemineraldensityreductionandmechanicalstrengthlosscouldbesignificantlyamelioratedbythehydrolyticproductsadditionindiet.
出版日期
2012年03月13日（中国期刊网平台首次上网日期，不代表论文的发表时间）
