摘要
ToinvestigatethechangesofthebiochemicalcompositionofAmericanshad(Alosasapidissima)eggsandlarvaeatembryonicandearlylarvalstages,sampleswerecollectedatdifferentdevelopmentstagesfromartificialfertilizationtotheendofyolkabsorptionincluding2h,12hand30hafterfertilizationandnewlyhatchedlarvaeincluding1and3daysafterhatching.Thecompositionoflipid,fattyacids,proteinandaminoacidswereanalyzed.Thecontentoftotalproteinexhibitedadecreasingtrendduringembryogenesisandlarvaldevelopment,andasignificantreductionwasdetectedafterhatching(P<0.05).Thetotallipidcontentremainedrelativestable.Asignificantreductionwasdetectedinalmostallaminoacidsafterhatchingexceptforglycine(P<0.05),whileasignificantdecreasewasfoundinthecontentofcysteine,proline,tyrosine,valine,isoleucine,leucineandphenylalanineduringtheyolk-sacphase(P<0.05).Ontheotherhand,allthegroupsoffattyacidsremainedstableduringtheperiodofembryogenesis.Butafterhatching,asignificantdecreasewasfoundinthecontentofC18:2n-6,C18:3n-6,SFAandratioofEPA/ARA(P<0.05),whileasignificantincreasewasfoundinthecontentofC18:3n-3,C20:4n-6,C22:6n-3andratioofn-3/n-6(P<0.05).Inconclusion,thecombineddatasuggestedthatAmericanshadutilizestheproteincontentaspreferentialenergysubstratesduringembryonicandearlylarvaldevelopmentswithsomespecificityintheconsumptionofdifferentaminoacids.
出版日期
2018年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
