Surface Characterization and in Vitro Blood Compatibility of Poly （Ethylene Terephthalate） Immobilized with Hirudin-中国期刊网

摘要 Poly(ethyleneterephthalate)(dacron,PET)filmswereexposedunderargonplasmaglowdischargewithdifferentglowsandinducedpolymerizationofacrylicacid(AA)inordertointroducecarboxylicacidgroupontoPET(PET-AA)assistedbyultravioletradiation(UV).HirudinimmobilizedPET(PET-HRD)filmswerepreparedbythegraftingofPET-AA,followedbychemicalreactionwithhirudin.ThesurfacestructureofthetreatedPETwasdeterminedbyX-rayphotoelectronspectroscopy(XPS).Thewettability,surfacefreeenergy,andinterfacefreeenergyofthefilmswereinvestigatedbycontactanglemeasurement.Thebloodcompatibilityofthefilmswasassessedbyplatelet-adhesiontestandfibrinogenconformationalchangemeasurementstoevaluatetheviabilityofthematerialsinbiomedicalengineering.Measurementbyscanningelectronmicroscopy(SEM)revealedthattheamountsofadhered,aggregatedandmorphologicallychangedplateletswerereducedonthehirudin-immobilizedPETfilms.Enzyme-linked-immunoassaymeasurementsthatdisclosedfibrinogenconformationalchangesshowedresultsconsistentwiththeplatelets’behavior.

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Surface Characterization and in Vitro Blood Compatibility of Poly （Ethylene Terephthalate） Immobilized with Hirudin

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Surface Characterization and in Vitro Blood Compatibility of Poly （Ethylene Terephthalate） Immobilized with Hirudin

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