简介：BACKGROUND:Olfactoryensheathingcelltransplantationcanactivateaxonalregenerationandenhancemyelinrepair,whicharebeneficialfortreatingdemyelinatingdiseases.OBJECTIVE:Toexploretheeffectsofolfactoryensheathingcelltransplantationonmyelinrepair,synaptophysinexpression,andmotorfunctioninaratmodelofexperimentalallergicencephalomyelitis.DESIGN,TIMEANDSETTING:Arandomized,controlledexperimentwasperformedattheLaboratoryofProvincialHospitalaffiliatedtoShandongUniversitybetweenAugust2006andSeptember2007.MATERIALS:Dibenzylamine(Hoechst33342),luxolfastblue,andrabbitanti-ratsynaptophysinantibodywereprovidedbySigma,USA.METHODS:OlfactoryensheathingcellsextractedfromneonatalWistarratswereculturedfor10-14daysandlabeledwithdibenzylamine.SpinalcordextractedfromahealthyguineapigwashomogenizedandequallymixedwithcompleteFreund'sadjuvant;thereafter,themixturewasintracutaneouslyinjectedintotwoposteriorvoixpedisofhealthymaleWistarratstoestablishmodelsofexperimentalallergicencephalomyelitis.Ratswererandomlydividedintoacontrolencephalomyelitisgroupandanolfactoryensheathingcelltransplantationgroup,36ratsineachgroup.Physiologicalsaline(2μL)oranolfactoryensheathingcellsuspension(2μL)wasseparatelyinjectedalonglateralcerebralventricleatday7post-modelinduction.MAINOUTCOMEMEASURES:Themigrationanddistributionofolfactoryensheathingcellswereobservedunderfluorescencemicroscopy;myelinrepairwasdetectedusinghematoxylin-eosinstainingandluxolfastbluestaining;synaptophysinexpressionwasmeasuredusingimmunohistochemicalstaining;motorfunctionwasevaluatedusingamotorfunctionscale.RESULTS:Olfactoryensheathingcellscouldsurviveinvivoandmigratetothedistalendofthetransplantfocusandspinalcord,andsurvived21days.Hematoxylin-eosinstainingandluxolfastbluestainingindicatedthatmyelininthetransplantationgroupwasintact,andtheinfla