简介:Inthisstudy,theentiremitochondrialDNA(mtDNA)controlregion(CR)ofPholisfangiwasamplifiedviapolymerasechainreactionfollowedbydirectsequencing.ThelengthofthemtDNACRconsensussequenceofP.fangiwas853bpinlength.Inaccordancewiththerecognitionsitesaswerepreviouslyreportedinfishspecies,themtDNACRsequenceofP.fangicanbedividedinto3domains,i.e.,theextendedterminalassociatedsequence(ETAS),thecentralconservedsequenceblock(CSB),andtheCSBdomain.Inaddition,thefollowingstructureswereidentifiedinthemtDNACRsequenceofP.fangi:2ETASsintheETASdomain(TASandcTAS),6CSBsinthecentralCSBdomain(CSB-FtoCSB-A),and3CSBsintheCSBdomain(CSB-1toCSB-3).ThesedemonstratedthatthestructureofthemtDNACRofP.fangiwassubstantiallydifferentfromthoseofmostotherfishspecies.ThemtDNACRsequenceofP.fangicontainedoneconservedregionfrom656bpto815bp.Similartomostotherfishspecies,P.fangihasnotandemrepeatsequencesinitsmtDNACRsequence.PhylogeneticanalysisbasedonthecompletemtDNACRsequencesshowedthattherewerenogeneticdifferenceswithinP.fangipopulationsofthesamegeographicaloriginandbetweenP.fangipopulationsofdifferentgeographicalorigins.
简介:Mercury(Hg)isoneofthecommonlyencounteredheavymetals,whichiswidespreadininshoresedimentsofChina.InordertoinvestigatethetoxicityofHgonmarineinvertebrates,westudiedtheeffectsofthedivalentmercuricion(Hg2+)(attwofinalconcentrationsof0.0025and0.0050mgL-1,preparedwithHgCl2)onmetallothionein(MT)content,DNAintegrity(DNAstrandbreaks)andcatalase(CAT)inthegillsandhepatopancreas,antioxidantenzymeactivitiesofsuperoxidedismutase(SOD),catalase(CAT)andglutathioneperoxidase(GPx),inthehemolymph,gillsandhepatopancreasoftheportunidcrabCharybdisjaponicaforanexperimentperiodupto15d.TheresultsindicatedthatMTwassignificantlyinducedafter3d,withapositivecorrelationwithHg2+doseandtimeinthehepatopancreasandanegativecorrelationwithHg2+doseandtimeinthegills.WhileCATinthehemolymphwasnotdetected,itincreasedinthehepatopancreasduringtheentireexperiment;SODandGPxinthethreetissueswerestimulatedafter12h,bothattainedpeakvalueandthenreducedduringtheexperimentalperiod.Meanwhile,DNAstrandbreakswereallinducedsignificantlyafter12h.TheseresultssuggestedthedetoxificationstrategiesagainstHg2+inthreetissuesofC.japonica.
简介:Aciladivaricata(Hinds,1843)andA.mirabilis(AdamsandReeve,1850)arecommonbenthicbivalvesinChina.Anumberofresearchershaveproposedthatthelatterspeciesisajuniorsynonymoftheformerspecies.Becauseofmorphologicalsimilarities,itisdifficulttodistinguishthesetwospeciesbasedonvisualexaminationonly.Forbetterunderstandingoftheirtaxonomy,themitochondrialCOIgenefragmentsoffiveindividualsofA.divaricatafromtheEastChinaSeaandsixindividualsofA.mirabilisfromtheYellowSeaweresequencedinthisstudy.ThephylogeneticrelationshipsoftheobtainedCOIsequences,togetherwithnineteensequencesofthreespeciesofthegenusNucula,wereanalyzed.Thepairwiseintra-andinter-specificdistancesfortheCOIsequencesrangedfrom0.002to0.017andfrom0.128to0.134,respectively,andnooverlapwasfound.Phylogenetically,A.divaricataandA.mirabilisformdistinctcladesandclusterintoasistertoallotherNuculaspecies.TheresultsindicatedthatA.divaricataandA.mirabilisaretwodistinctspecies.Thedifferencesinthemorphologyanddistributionbetweenthetwospecieswerebrieflydiscussed.
简介:Inthispaper,wetooktheleadinstudyingonspecificityofthemicrosatelliteDNAlociandapplicabilityofmicrosatelliteDNAprimersinprotozoa.Inordertostudycharactersofmicrosatellitesinfree-livingprotozoa,eightmicrosatellitelociprimersdevelopedfromTrypanosomacruzi(MCLE01,SCLE10,MCLE08,SCLE11,MCLF10,MCLG10,MCL03,MCL05)wereemployedtoamplifymicrosatelliteinfourfree-livingprotozoa,includingBododesignis,EuglenagracilisFACHB848,ParameciumbruziseandTetrahymenathermophilaBF1.IntheamplificationsystemsofP.bruzise,fourloci(SCLE10,SCLE11,MCLF10,MCL03)wereamplifiedsuccessfully,andfouramplificationfragmentswereinpropersize.IngenomeofE.gracilisFACHB848,fiveofeightprimersbroughtfiveclearamplificationbands.InB.designis,three(No.4,5and7)ofeightlociproducedclearandsharpproductswithoutstutterbands,whereasnobandsappearedinT.thermophilaBF1.Further,eight300-500bpamplificationfragmentswereclonedandsequenced.Nevertheless,allsequencedproductsdidnotcontaincorrespondingmicrosatellitesequence,althoughBodoisinthesameorderandhasthenearestphylogeneticrelationwithTrypanosomaamongthesefourspecies.Thus,themicrosatelliteDNAprimerscannotbeappliedamongorderormorefartaxa,andthespecificityofmicrosatelliteDNAisveryhighinprotozoa.TheresultsofthisstudywillcontributetoourunderstandingofmicrosatelliteDNAinprotozoa.
简介:Saccharina是最重要的无热水设备生活水兵褐之一海藻的类。在这研究,我们分析了S的transcriptome。装饰用的梨树,它属于1000植物(OneKP)工程,由使用定序技术的下一代的高产量的DNA。未加工的数据的大约5.16GB被产生,并且有454bp的平均长度的65536脚手架与肥皂denovo汇编方法被装配。总共,19040unigenes被强风识别;25734脚手架被聚类进37基因本体论功能的组;6760脚手架被分类进25个轮牙范畴,以及被分到306条KEGG小径的2665脚手架。unigenes的多数比另外的cyanobacteria,海洋的硅藻,和植物包括棕色的水藻和硅藻展出了更多的类似到水藻。Saccharina装饰用的梨树有突出的能力积累象Br那样的卤素,我从海水经由halogenation处理。我们在S获得了42不同钒依赖者haloperoxidases(vHPO)。装饰用的梨树transcriptome数据,包括钒依赖者iodoperoxidase(vIPO)的5个片断和钒依赖者bromoperoxidase(vBPO)的37个片断。识别fulllengthS的复杂分析。装饰用的梨树vBPO1和S。装饰用的梨树vBPO2在在海洋的海藻的vBPOs和vIPOs之间的棕色的水藻和强壮的关系的种类之中揭示了vBPO的重要性。这研究将提高我们生物特征的理解和S的经济价值。装饰用的梨树种类。
简介:从中国福建沿海某一污染滩涂的沉积物中筛选到一株具有较强拮抗水产病原菌的生防细菌LHB02。采用通用引物,对LHB02的16SrRNA片段进行扩增,获得1511bp的DNA系列,基于该DNA系列,结合细菌形态学、生理生化特征,鉴定LHB02为一株枯草芽孢杆菌。对LHB02进行了抗菌试验、抗菌谱检测和发酵条件优化研究,结果表明,LHB02对哈维氏弧菌、溶藻弧菌、鳗弧菌等有很强的拮抗作用,其优化后的发酵条件为:KB培养基(蛋白胨20g,丙三醇10mL,K2HPO41.5g,MgSO4.7H2O1.5g,H2O1000mL);温度28°C,pH值7.0,培养时间36h,接种量1.5%。
简介:我们曾报道了短梗霉菌产生的高纤维素酶产量98。在这项研究中,羧甲基纤维素酶(CMCase)在培养的细胞。短梗霉98的纯化至均一,与酶的最大产量为4.51U(mg蛋白)-1。SDS-PAGE分析表明,纯化的酶的分子量为67.0kda。具有相当的敏感性为40℃纯化的酶的最适温度,比从其他真菌的cmcases低得多。该酶的最佳pH值为5.6,和活动的个人资料被稳定在一个范围内的酸度(pH5,0-6.0)。这种酶被激活Na+,Mg2+,Ca2+,K+,Fe2+和Cu2+,然而,它是由Fe3+,Ba2+,Zn2+,Mn2+和银离子抑制。公里和纯化的酶的Vmax值4.7mgml-10.57pmolL-1min-1(mg蛋白)1,分别。只有大小不同的低聚糖,羧甲基纤维素(CMC)释放与纯化的酶水解后。该基因编码的酶是A.霉98个克隆,其中包含一个开放阅读框(eu978473)意义。推导的蛋白质含有酶超家族的保守结构域(糖基水解酶家族5)。的N-末端氨基酸序列的纯化的酶是m-a-p-h-a-e-p-q-s-q-t-t-e-q-t-s-s-g-q-f,这与从克隆的基因推导一致。这表明,纯化的酶是由克隆纤维素酶基因在酵母编码。