简介：Whitespotsyndromevirus(WSSV)isoneofthemajorshrimppathogenscausinglargeeconomiclossestoshrimpfarming.Inanattempttoidentifytheenvelopeproteinsinvolvedinthevirusinfection,purifiedWSSVvirionsweremixedwiththreeantiseraagainstWSSVenvelopeproteins(VP39,VP124andVP187),individually.Andthentheywereinjectedintramuscularlyintocrayfish(Procambarusclarkii)toconductinvivoneutralizationassays.TheresultsshowedthatforgroupsinjectedwithvirionsonlyandgroupsinjectedwiththemixtureofvirionsandantiserumagainstVP124,thecrayfishmortalitieswere100%and60%onthe8thdaypostinfection,individually.ThevirusinfectioncouldbedelayedorneutralizedbyantibodyagainsttheenvelopeproteinVP124.QuantitativePCRwasusedtofurtherinvestigatetheinfluenceofthreeantiseradescribedaboveonthevirusinfection.TheresultsshowedthattheantiserumagainstVP124couldrestrainthepropagationofWSSVincrayfish.AlloftheresultssuggestedthattheviralenvelopeproteinVP124playedaroleinWSSVinfection.

简介：-thymosins,高度保存的肽的一个家庭，起一个重要作用在愈合创伤，angiogenesis，抗菌剂过程和抗病毒的免疫。三新奇-thymosin-repeat蛋白质，命名mjthm4，mjthm3和mjthm2，从抑制用表示顺序标签(EST)从Marsupenaeusjaponicus被克隆减少性的杂交。拥有的mjthm4，mjthm3和mjthm2cDNAs打开编码了166，128和90的读的框架氨基酸残余多肽并且包含了四，三和二-thymosin肌动朊绑定模块分别地。强风分析证明mjthm4，mjthm3和mjthm2与已知的无脊椎的多重复-thymosins分享了高相同。这些蛋白质无所不在地检验纸巾在所有被表示，并且transcriptional层次在肠是最高的。进一步的调查表明mjthm4，mjthm3和mjthm2是显著地起来调整的在WSSV感染以后的6h。而且当mjthm4transcriptional层次没显示变化时，mjthm3和mjthm2层次在病毒抵抗的虾减少了。结果显示mjthm4，mjthm3和mjthm2是新奇多重复-thymosin相当或相同事物，与WSSV感染有一种靠近的关系，并且可能贡献在虾的主人防卫或病毒侵略相互作用的更好的理解。