简介：ThebindingofCo(bpy)2dppz3+tocalfthymusDNAwasinvestigatedbyusingabsorptionandemissionspectroscopy,DNAmeltingtechniques,cyclicvoltammetry,viscosityandelectro-phoresismeasurements,wherebpyis2,2’-bipyridyl,dppzisdipyrido[3,2-o:2’,3’-c]phenazine.Thebindingcompoundshowsabsorptionhypochromicity,fluorescenceenhancement,andincreasingofDNAmeltingtemperatureandthespecificviscosity.CVmeasurementshowstheshiftsinoxidation-reductionpotentialandchangeinpeakcurrentwithadditionofDNA.ThecompoundisalsoshowntobemoreefficientphotosensitisersforstrandbreaksinplasmidDNA.

简介：TheeffectofCdionsonsalmonspermDNAwasstudiedbymeansofcirculardichroism(CD),Ramanspectroscopy,X-rayphotoelectronspectroscopy(XPS)andfluorescencespectroscopy.TheCDspectralandfluorescentprobe-acriflavineresultsindicatethattheDNAunderwentaconformationchangeupontheadditionofCdions.XPSandRamanstudiesrevealthatthereexistedinteractionsbetweenCdionsandthephosphategroupsoftheDNA.Inaddition,anewbandappearedat803cm-1intheRamanspectra,whichcanbeattributedtocharacterizing"marker"bandofA-DNA.ItisconcludedthatCdionscanbecoordinatedbythephosphategroupsoftheDNAandinducetheconformationchangesoftheDNAfromB-DNAtoA-DNA.

简介：HydrolysisofDNAisanimportantenzymaticreaction,butitisexceedinglydifficulttomimicinthelaboratorybecauseofthestabilityofhydrolysisofDNA.Inthispaper,thecleavageactivityofcomplexesformedbetweenCu(Ⅱ)andfourdifferentaminoacidoraminoacidmethylesteronDNAisstudiedbygelelec-trophoresis.ItisfoundthatDNAcouldbecleavedbyCu(Ⅱ)-L-HisandCu(Ⅱ)-L-Hismethylestercomplexesandtheefficiencyofcleavageislargelydependentonthemetalion-to-ligandratio.FurtherexperimentsshowthatthecleavageofDNAmediatedbyCu(Ⅱ)-L-HiscomplexesoccursviaahydrolyticmechanismandtheactivechemicalspeciesthataffectsDNAcleavageisproposedtobeMI2H+andML2H22+.

简介：Adetectionofanthracyclineantitumordrugdaunomycin(DNR)reactingwithDNAinsimulatemetabolisminvitrohasbeenmade.ItwasfoundthatDNRcouldreactwithDNAtoformDNR-DNAadducts.TheadductcompositionsofDNRwithfishspermDNAandthermallydenaturatedDNAweredetermined.TheequilibriumassociationconstantKofDNRwithfishspermDNAis1.98×10^5L/molandthatofDNRwithdenaturatedDNAis2.29×10^4L/mol.Semiquinonefreeradicals,metabolicproductsofDNR,candestroybothfishspermDNAanditsthermallydenaturatedDNA.ItisverifiedbyhyperchromiceffectincreaseobservedinUVspectrumandAFMexperiments.ThemechanismofDNAdegradationhasalsobeeninvestigated.Resultsobtainedallowonetoexplainthereasonofsideeffectofanthracyclinedrugandgivethewaytodepress,whichwereofclinicalsignificance.

简介：AvohammetricstudyoftheinteractionofneutralRed(NR)withDNAatagoldelectrodeinaphosphatebuffersolutionisdescribed.AfteraddingDNAinanNRsolution,thereductionpeakcurrentofNRdecreases.ThebindingmeehahismsofNRtoDNAindifferentpHrangesaredifferent.ThereductionpeakpotentialofNRinapH7.0phosphatebuffersolutioninthepresenceofDNAshiftspositively,indicatingthatthebindingofNRtoDNAisintercalationaction,butatpH=6.0thereductionpeakpotentialofNRshiftsnegatively,indicatingthatthebindingofNRtoDNAiselectrostaticaction.TheformedcomplexesareDNA-NRwhen[NR]/[DNA]<0.18andDNA-3NRwhen[NR]/[DNA]>0.35,respectively.

简介：MtDNAwassuccessfullyextractedfromtenindividualbones(femurs)inthetombsofancientJushiinTurfanbasin,datedbacktotheyearabout3000-2500yearsago.Bymeansoffouroverlappingprimers,wegotnucleotidesequenceofthe218bplength.AncientmtDNAwasanalyzedbythesequencingofhypervariableregionⅠofthemtDNAcontrolregion.Theresultshowsthat9haplotypeswith24polymorphicsiteswereobtained.ThephylogeneticanalysisindicatedthatMongoliansandAltaiarethepopulationgeneticallyclosesttotheJushigroupsandJushimtDNApoolbeinganadmixtureofeasternAsianandEuropeanlineages.SoourpreliminarydataimplythatanancientminglingofEuro-AsianpopulationhadexistedinTurfanbasinpriortotheearlyIronAge.

简介：Rheumatoidfactors(RFs)arethecharacteristicautoantibodiesofrheumatoidarthritis.Recentresearchesinourlaboratoryshowedthattheimmobilizedsingle-strandedDNA(ss-DNA)immunoadsorbentcanselectivelyremoveRFsfromtheserumofpatients.Inthepresentpaperarestudiedthemodificationofargininine,tryptophan,lysineresiduesandcarboxylterminusofIgGRF,whichwasseparatedfrompatients′serum,with1,2-cyclohexanedione(CHD),N-bromosuccinimide(NBS),pyridoxal5′-phosphate(PP)and1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC)respectively,andtheireffectsontheadsorptioncapacityoftheimmobilizedss-DNAimmunoadsorbentforIgGRF.Afterthespecificmodification,thecorrespondingadsorptioncapacitiesoftheadsorbentswerechangedfrom48%,46%,44%and54%to84%,14%,21%and81%,respectively.Theseresultsindicatethattheelectrostaticorionic-bondingisessentialfortheinteractionbetweenss-DNAandIgGRF.

简介：TheroleplayedbyDNAinmolecularbiologyisclearlyrecognizedtobevitaltolifeonthisplanet.8-oxo-7,8-dihydro-2deoxyguanosine(=8-OHdG),isprobablythemostimportantproductof"oxidativestress”inDNA.ItsconcentrationinDNAis,infact.aquantitativeanalysisofthedegreeofDNAdamagethatanorganismhasundergone.Duetotheimportanceof8-OHdGofnucleicacidginmutagenesis,carcinogenesisandaging,numerouschemicalandbiologicalinvestigationshavebeenmadeonthissubjectinthepasttime.Kuchinoandco-workershavefoundthat8-OHdGresidueinDNAismisreadingduringtheprocessofDNAreplication.Recently,somereportshavebeenpresentedonhigh8-OHdGlevelsinpatientssufferingfromvariousdiseasessuchaschronichepatitis,Fanconisanemia,diabetesmellitusandHelicobacterpyloriinfections.Asaresult,8-OHdGisausefulmarkerforthestudyofDNAdamagearisingfromreactiveoxygenspeciesandisofgreatsignificanceforcancerresearch.The8-OHdGlevelsinDNAcanhelpunderstandthemechanismofcarcinogensandleadtomoreeffectivetreatmentsformanydifferenttypesofcancer.Forthesereasons,ananalysisof8-OHdGwithquickness,lowcostandhighaccuracyisrequired.

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简介：Thedye-dopedsilicananoparticlescanbeusedasnanobiosensorsthatareabletorecognizeanddetectspecificDNAsequence.Inthispaper,sphericalnanosizedluminol/SiO2compositeparticleshavebeensynthesizedwithreversemicellsviahydrolysisoftetraethylorthosilicate(TEOS)inthemicroemulsion.ThenanoparticlesweremodifiedwithchitosanandusedtolabelDNA,formingtheDNAprobewhichwasusedtohybridizewithtargetDNAimmobilizedonaPPymodifiedPtelectrode.Thehybridizationeventswereevaluatedbyelectrogeneratedchemiluminescence(ECL)measurementsandonlythecomplementarysequencecouldformadouble-strandedDNA(dsDNA)withDNAprobeandgivestrongECLsignals.Athreebasemismatchsequenceandanon-complementarysequencehadalmostnegligibleresponses.Duetothelargenumberofluminolmoleculesinsidesilicananoparticles,theassayallowsdetectionatlevelsaslowas2.0×10^-12mol/LofthetargetDNA.TheintensityofECLwaslinearlyrelatedtotheconcentrationofthecomplementarysequenceintherangeof5.0×10^-12—1.0×10^-9mol/L.

简介：本文报导了作者们编制的分子波函数绘图程序JDPLOT,利用该程序可绘制各类原子轨道、分子轨道、轨道电子密度截面等值图,以及它们的截面立体透视图。文中给出了简单示例。

简介：新奇建筑群[公司(phen)2HPlP]Cl3[phen=phenanethroline，HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][l，10]phenanethroline]被元素的分析综合了，在结构上描绘了，紫外，红外和1HNMR光谱学。有小牛胸腺DNA(CTDNA)的建筑群的相互作用被学习了使用吸收和排放光谱学，DNA融化技术和周期的voltammetry。混合物看吸收hypochromicity，荧光改进和DNA融化温度增长是否有约束力到CTDNA。CV测量与DNA的增加在山峰电流在减小潜力和一个变化显示出移动。这些结果证明混合物插入到DNA基础对的地席。山峰潜力的移动显示在建筑群和DNA之间的离子相互作用模式。到DNA的混合物的有约束力的常数是4.37灳慬瑮?郥誸???蚰铧?髧??螻?趤??藥???藥薗郧芀漀椭吗？？

简介：ＳＴＵＤＹ　ＯＮ　ＴＨＥ　ＲＥＬＡＴＩＯＮＳＨＩＰ　ＢＥＴＷＥＥＮ　ＭＥＬＴＩＮＧ　ＰＯＩＮＴ　ＡＮＤ　ＡＣＩＤ　ＤＥＮＡＴＵＲＩＮＧ　ＡＣＩＤＩＴＹ　ＯＦ　ＤＮＡ　ＢＹ　ＵＬＴＲＡＶＩＯＬＥＴ　ＳＰＥＣＴＲＯＭＥＴＥＲＳＴＵＤＹＯＮＴＨＥＲＥＬＡＴ...

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简介：对硝基取代甲烷的硝基式和酸式两种异构体,用CNDO/2方法进行了计算.结果发现这类化合物的C—N间键级函数(YCN)与离解能(DCN)、键长;H—O间键级函数(YOH)、氢原子极化率（PH）与酸性、熔点;能量差（△E）与平衡常数之间具有某种相关性.对硝基甲烷的负离子的几何构型和成键情况进行了讨论,负离子为平面构型,C—N间约为1.6级键.

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简介：用二次量子化方法讨论了ＨＣｌ分子在激光场中的多光子激发，还包括对ＨＣｌ分子伸缩振动能级的计算，跃迁概率随外场频率的变化及随时间的变化(取光场强度１０－８Ｗ／ｃｍ２)。

简介：通过分子轨道理论和杂化轨道理论推断出较稳定的白藜芦醇分子是平面型分子，然后根据白藜芦醇分子结构特点计算了该化合物的一种稳定异构体的转动惯量，用矢量合成法计算了其电偶极矩，为微波辅助白藜芦醇萃取理论研究提供转动惯量和电偶极矩的数据．