简介：Anovelbiphenylside-chaineddiaminewithalkyloxyspacerandalkyloxytail,4'-butoxy-4-(3,5-diaminobenzoyloxy)hexyloxybiphenyl(C6BBC4),wassynthesizedandcharacterizedbyFTIRand1HNMR.Aseriesofpolyimide(PI)filmswithdifferentsidechaincontentswassynthesizedfrombiphenyltetracarboxylicdianhydride(BPDA),4,4'-oxydianiline(ODA)andC6BBC4,andtheirthermalproperties,opticaltransparencyandliquidcrystalalignmentproperty,andsoon,wereinvestigatedbythermogravimetricanalysis(TGA),differentialscanningcalorimetry(DSC),crystalrotationmethod,polarizingmicroscopyandUV-Visspectra.Withthecontentofsidechaineddiamineincreasingfrom0to100%,theηinhofpoly(amideacid)(PAA)decreasedrapidlyfrom3.0to0.51,theglasstransitiontemperature(Tg)ofpolyimides(PIs)decreasedfrom274℃to203℃andtheVis-lighttransmittanceofalignmentfilmssubstantiallyincreased.Asforthealignmentproperties,thepretiltangleofPIalignmentfilmswithoutrubbingcouldreach90°whenthecontentofC6BBC4was60%ormore.Atthesametime,thealignmentstabilitycouldmeettheindustrialrequirements.Itwasconsideredthatthealkoxyspacer,thebiphenylunitandthealkoxytailmayallplayimportantrolesinachievingalargepretiltangleevenupto90°,andtheintroductionofonlythealkoxyspaceroralkoxytailcanalsoimprovethepretiltangle,butnotbymuch.

简介：Twosolubleisomerizedpolyimides(PIs)synthesizedfrom2,2'-bis(3,4-dicarboxyphenyl)hexafluoropropanedianhydride(6FDA)witheither2,2'-dimethylbenzidine(2,2'-DMB)or3,3'-DMBwereinvestigatedbymeansofsize-exclusionchromatographycoupledwithmulti-anglelaserlightscattering,aviscometerandarefractiveindexdetectorintetrahydrofuran(THF)withtetrabutylammoniumbromide(TBAB)at35℃.Thecorrespondingparametersrelatedtoconformationsαandν,evaluatedfromthescalingrelationships[η]=KηMαandRg=KgMν,respectively,were0.66±0.01and0.55±0.02forpoly(6FDA/3,3'-DMB),and0.67±0.01and0.56±0.01forpoly(6FDA/2,2'-DMB),indicatingarandomcoilconformationforboththesamplesinthismobilesystem.ThepersistencelengthlpandshiftfactorML(relativemolecularweightperunitcontourlength)wereestimatedfromtherelationshipbetweenintrinsicviscosityandmolecularweightforthewormlikecylindermodelproposedbyBohdanecky.BothlpandMLshowedthatthetwoPIsinTHFareflexiblechainsandexhibitsomelocalrigiditytosomeextent.

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简介：styrenictriblock共聚物(SBC)的导致温度的分子的链运动，即polystyrene-block-polybutadiene-block-polystyrene(SBS)并且polystyrene-block-poly(ethylene-co-1-butene)-block-polystyrene(SEBS)，被内在的荧光方法学习。为SBS，内在的荧光方法获得的B块和S块的玻璃转变温度(Tgs)在对微分扫描热量测定大小(DSC)的好同意。在SEBS的情况中，一个isoemission点在提高的温度在大约310nm被遵守，建议在单体和excimer排放之间的细微变换。在这个基础上，SEBS的分子的链运动被荧光紧张和excimer/monomer荧光比率监视。除S块和EB块的Tgs以外，一个融化的点(Tm)弱在EB水晶块被内在的荧光明白地决定。而且，融化的过程直接导致了在接口并且因而PS片断松开的忽视，这被发现excimer的数量的减小。合理机制被建议在加热之上描述分子的链运动和SEBS的阶段转变。而且，非放射的过程的明显的激活精力上的温度的影响(吃)在S的Tg附近，块在B或EB块的Tg附近是比那强壮得多的。

简介：二个Schiff底转移忍受morpholine戒指的方面链的金属建筑群，这被报导了被综合并且描绘，并且有一样的二建筑群基于代理人但是不同金属离子在这份报纸在p-nitrophenylpicolinate的催化水解作用被用作假装的hydrolase。PNPP催化水解作用的机制被结果建议并且支持光谱分析和运动计算。一个运动数学模型，适用于计算运动并且PNPP催化水解作用的热力学参数，在建议的机制的基础上被建立了。学习的结果证明二建筑群在PNPP催化水解作用的PNPP催化水解作用，和率举办一项好催化活动随缓冲区解决方案的pH值的增加被增加并且由建筑群的金属离子的极化效果影响了。

简介：Amain-chainliquidcrystallineionomer(MLCI)containingsulfonicgroupwassynthesizedbyaninterfacialcondensationreaction.TheMLCIwasblendedwithpolybutyleneterephthalate(PBT)andpolypropylene(PP).MLCIinteractedwithboththedispersed(PP)phaseandthematrix(PBT)phasetomodifytheinterfacialinteractionofPBTandPP.Differentialscanningcalorimetry(DSC),scanningelectronmicroscopy(SEM)andFTIRimagingsystemanalysisdemonstratedthesignificanceofinterfacialinteractioninthepolymerblends.MLCIbroughtaboutgoodadhesionattheinterfacial,whichreducedthedispersephasesizeandenabledafinePPphaseatmatrix.ThemechanicalpropertiesoftheternaryblendswereimprovedwhenaproperamountofMLCIwasadded.Thiswasattributedtoenhancedadhesionattheinterface,whichinvokedbettermechanicalpropertiesintheblends.

简介：TofindoptimalconditionsforexpressingthesolubleformofsFv-2F3andtostudythepurificationandpropertyofitsderivativeSe-sFv-2F3,thepreferredexpressionconditionswereinvestigatedbymeansoforthogonaldesign.Thesecultureconditionsincludedincubationtemperature,inducerconcentration,inductiontimeandcellconcentration.TheevaluationofexpressionwasaccomplishedbytheanalysisofwholecelllysatesandtheyieldofsolublesFv-2F3wascalculatedaccordingtotheanalysisofProfinder(FTI-500,Pharmacia).Thepurificationprocedurewascarriedoutviaatwo-steppurificationprocedureconsistingofion-exchangechromatography,followedbyimmobilizedmetalaffinitychromatography(IMAC).TheantioxidantefficacyofSe-sFv-2F3wasdemonstratedbythedeterminationofthecontentofthemainproductoflipidperoxidation,MDA,theviabilityofcellsandtheactivityofLDH.WeobtainedthepreferredcultureconditionstogrowtheengineeredbacteriaandtheprocedureforpreparingsolublesFv-2F3andconfirmedtheantioxidantefficacyofSe-sFv-2F3.

简介：Inordertoenhancetheglutathioneperoxidase(GPX)catalyticactivityoftheselenium-containingsingle-chainvariablefragments(Se-scFv),anovelhumanscFvwasdesignedonthebasisofthestructureofhumanantibodyandoptimizedviabioinformaticsmethodssuchashomologoussequenceanalysis,three-dimensional(3D)modelbuilding,binding-siteanalysisanddocking.TheDNAsequenceofthenewhumanscFvwassynthesizedandclonedintotheexpressionvectorpET22b(+),thenthescFvproteinwasexpressedinsolubleforminEscherichiacoliBL21(DE3)andpurifiedbyNi2+-immobilizedmetalaffinitychromatography(IMAC).TheserineresidueofscFvintheactivesitewasconvertedintoselenocysteine(Sec)withthechemicalmodificationmethod,thus,thehumanSe-scFvwithGPXactivitywasobtained.TheGPXactivityoftheSe-scFvproteinwascharacterized.ComparedwithotherSe-scFv,thenewhumanSe-scFvshowedsimilarefficiencyforcatalyzingthereductionofhydrogenperoxidebyglutathione.ItexhibitedpHandtemperaturedependentcatalyticactivityandatypicalping-pongkineticmechanism.