简介:目的研究滁州市C群流脑的流行特征与控制措施,为今后C群流脑防控工作提供科学依据。方法对2004~2005年度流脑个案调查、实验室检测结果进行分析。结果28例流脑患者中检出5例C群Nm,病例的密切接触者中检出4株C群Nm;在地区分布中,南谯区乌衣镇中学发生5例C群流脑为局部爆发,其余为散发病例;病倒中学生占71.42%,10~20岁年龄组占71.42%;有A群流脑疫苗免疫史占82.14%,有5例流脑A+C二价疫苗免疫失败。结论C群流脑病例来势凶猛,病程进展快,病死率高;接种A群流脑疫苗不能阻止C群流脑流行;为防止发病控制疫情扩散,应对20岁以下人群和在校的大、中专学生等易感群体提供免疫保护。
简介:Triglyceride-richhpoprotein(TRL)remnantshavebeenimphcatedbybothclinicalandlaboratorystudiesinthepathogenesisofatheroselerosisandthrombosis.Weuseannewimmunoseparationmethod,providedbyJapanImmunoresearchLaboratories,todeterminetheremnant-hkeparticles(RLP)ofhpeproteins.IsolationofRLPfromserumisachievedusinganimmunoaffmitygelcontainingspecificanti-humanapoA-IandapoB-100(JI-H)monoelonalantibodies,wheretheformerrecognizesHDLandnewlysecretedapoA-I-containingchylomicrons,thelatterrecognizesLDLandmajorityofVLDL.AsaresultofthespecificityoftheJI-Hantibody,themajorityoftheapoB-48-containingchylomicrons,andcertainapoE-enrichedapoB-100-containingVLDLaxenotrecognized.TheselipoproteinsarenotcapturedbytheimmunoaffinitygelandarethusisolatedintheunboundRLPfraction,whichispredominantlymadeupofTRLhavingremnant-hkeproperties.ThecomponentsoftheunboundedfractionaxetheremnantsofCMandVLDLs,verifiedbyultracentrifugationandlipoproteinselectrophoresisinagarosegels.WeestablishedaconvenientapproachtomeasureRLP-cholesterolontheHitachi7150biochemicalanalyser.Thewithin-runandrun-to-runimprecision(CV)oftheassaywas2.75%and11.48%respectively.ThelinearoftheRLP-cinourassaywas0-180mg/dl.Wechose30normallipidslevelforthecontrolgroup(15male&15female),themedianfastingserumRLP-cconcentrationwas8.12±4.88mg/dl.WefredthatRLP-cconcentrationweresignficantlycorrelatedwithTG,VLDL-c,HDL-c&apoE(r=0.765,0.511,-0.378,0.241respectively,P<0.01);andnosignficantcorrelationwassubsequentlyobservedbetweenRLP-candLDL-c.Inourstudy,themedianfastingRLP-cconcentrationweresignificantlyhigherin91patientswithcoronaryarterydisease(CAD),in15patientswithnon-insulindependentdiabetesmeUitus(DM),andin45patientsundergoinghemodialysiswithend-stageretinaldisease
简介:摘要回顾性分析40例肾病患儿的胱抑素C、肌酐和尿素检测结果,得出胱抑素C与肌酐和尿素检测结果具有一致性,并且其灵敏度更高。
简介:摘要目的根据糖尿病患者胰岛素、C肽释放试验的结果判定胰岛β细胞的功能,从而进一步将糖尿病分型及选择恰当的治疗方法。方法糖尿病患者,男251例,平均年龄19~78岁,女259例,平均年龄12~70岁。抽取静脉血1mL(空腹、60分钟、120分钟、180分钟各1次,凡空腹血糖<13.3mmol/L者给予75g葡萄糖口服,凡空腹血糖>13.3mmol/L者给予100g馒头餐),并同步应用磁酶联免疫分析仪进行测定。结果年轻起病的糖尿病人尤其20岁以下男子以胰岛β细胞功能衰竭为主,为Ⅰ型糖尿病;20~40岁年轻病人胰岛β细胞功能以延迟为主,为型糖尿病;40岁以上糖尿病发病率逐渐增高,胰岛β细胞功能以延迟为主,为Ⅱ型糖尿病;部分肥胖病人为高胰岛素血症。结论根据试验结果可将糖尿病明确分型,合理的指导临床用药,极大方便了患者及临床医生。