简介:Objective:Toinvestigatetheeffectofbreast-conservationtherapyinearlystagebreastcancer.Methods:Atotalof234earlystagebreastcarcinomapatientsreceivedbreastconservingtreatmentinourhospital.Aftertheoperation,theyunderwentadjuvantchemotherapyandradiotherapy.Allofthesepatientsdesiredtopreservetheirbreasts.Results:Aftermedianfollow-upof29.46months(rangefrom3to100months),3caseshadlocalrelapseand8caseshaddistantmetastasis.Theoverallsurvivalrateof5yearwas96.7%,andthediseasefreesurvivalrateof5yearwas87.85%.Conclusion:Forearlystagebreastcarcinomapatients,classicquadrantectomy,axillarydissectionandpost-operativeadjuvantchemotherapyandradiotherapyleadtoexcellentlocalcontrolandgoodsurvival.
简介:Forelectronicmicroscopicobservation,wefoundSSV-transformedNIH3T3cellsweredifferentfromnon-transformedcells.InSSV-transformedNIH3T3cellsnucleicytoplasmaratiowasincreasedandincytoplasmatheribosomes(polyribosomeswereattachedtotheswollenroughendoplasmicreticulum.Itwaslikelythatribosomeswerelinedtogetherfunctionallyandstructionallytoproducespecificprotein(PDGF-likeprotein).
简介:Objective:ToexploretheeffectsofnuclearM-CSFontheprocessoftumorigenesis.Methods:FunctionalpartofM-CSFcDNAwasinsertedintoaneukaryoticexpressionplasmidpCMV/myc/nuc,whichcanaddthreeNLStotheC-terminaloftheexpressedproteinanddirecttheproteinintothecellnuclei.TheconstructedplasmidwastransferredintoNIH3T3cellsandthecellcloneswereselectedbyG-418selection.CellclonesstableexpressingtargetproteinwereidentifiedbyRT-PCR,ABCimmunohistochemistryassayandWesternblot.Cellgrowthkineticsanalysesthroughgrowthcurves,celldoublingtime,MTTtestandanti-senseoligodeoxynucleotide(ASODN)inhibitingcellgrowthtestwereperformedtoidentifycellsproliferationpotential.Results:Thetransfectedcellsshowedelevatedproliferationpotentialoverthecontrolcells.Conclusion:AbnormalappearanceofM-CSFinnucleuscouldenhancecellproliferation,whichsuggeststhatcytokineisoformswithincellnucleusmightplaytranscriptionfactor-likerole.
简介:Objective:ToconstructamutantpEGFP-hTERTexpressionvector,toobserveitssteadyexpressionintransfectedhumanbladdercarcinomacelllineT24anditsroleinmolecularregulatorymechanismsoftelomerase,andtoprovideanewtargetgeneforbladdercancer.Methods:PCRamplificationwasperformedbyusingprimersbasedontheknowngenesequenceofhTERT.PCRproductionwasclonedintoplasmidpGEMT-TeasyandthesequenceofmutanthTERTgenewasanalyzed.ArecombinantmutanthTERTvector(pEGFP-hTERT)wasconstructedattheEcoRIandSalIsitesofthepEGFP-C1vector.AftertransfectingthefusiongeneintobladdercarcinomacelllineT24bycalciumphosphate-DNAcoprecipitation,thesteadyexpressionofGFP-hTERTfusionproteinwastestedbyfluorescentlightmicroscopy.TheproliferationchangesofbladdercarcinomacelllineT24weredetectedbylightmicroscopyandsenescencecorrelatedβ-galactosidasestaining.Results:IdentificationofpEGFP-hTERTbyenzymedigestionshowedthatmutanthTERTfragmenthadbeenclonedintoEcoRIandSalIsitesofthepEGFP-C1vector.ThesteadyexpressionofGFP-hTERTfusionproteinwaslocalizedinthenucleusoftransfectedcells.Expressionofsenescence-associatedβ-galactosidaseintransfectedcellsgraduallyincreasedwithextendedculturedtimeandcellgrowthwassuppressed.Conclusion:Themutant-typehTERTgenesuppressestheproliferationofbladdercarcinomacelllineT24bycompetitiveeffectontelomeraseactivity.ThissuggeststhathTERTgenemightbeasuitablegenetargetforbladdercancertherapy.
简介:TheeffectofTPA,apotenttumorpromoter,onSSV-NIH3T3cellsinserum-freemediumwasinvestigated.TPAstimulatedDNAsynthesisofSSV-NIH3T3cellsonthethirddayofcultureinSFM.InSDS-PAGFofmediumconditionedbyTPA-treatedSSV-NIH3T3cells(inSFM+TPA),theamountsoffourproteinsof31.0Kd,28.5Kd,25.5Kdand13.5Kdstrikinglyincreasedoverthatofnon-TPA-treatedcounterpart(inSFM).ThePDGF-likeactivitywasalsodetectedinCMofSFM+TPA.WheninsulinandEGFweredrownofftheSFM+TPA(SFM-Ins-EGF+TPA),TPAlostitsabilitytostimulateDNAsynthesisofSSV-NIH3T3cellsonthethirddayandSDS-PAGEoftheconditionedmediumshowedthattheamountsofthefourproteinsnotedabovegratelyreduced.However,cellsinSFM-Ins-EGF+TPAwereinalmostthesamegrowthconditionascellsincompleteSFM+TPAonthethirddayofculture.Resultswerediscussedinthepaper.
简介:客观:为了改进倔强的脸中部的外部T房间non-Hodgkin鈥檚的功效,有L天门冬氨酰胺酶(LASP)的淋巴瘤(MPTC-NHL)基于抢救化疗。方法:有倔强的MPTC-NHL的21个病人被分析,11patients(LASP组)收到了L天门冬氨酰胺酶基于的抢救化疗由L天门冬氨酰胺酶,长春新碱和dexame-thosone组成。没有L天门冬氨酰胺酶,10个病人(控制组)收到了抢救联合化疗。结果:完全的宽恕率为LASP组是45.6%,0.0%为控制组织(P<0.05)。全面反应率(CR+PR)为LASP组是63.6%,10.0%为控制组织,分别地(P<0.05)。2年的幸存率为LASP组是45.5%,0.0%为控制组织(P<0.05)。LASP的主要不利效果是白细胞减少,浆液bilirubin和多糖症的举起。结论:LASP基于的初步的临床的学习表演抢救化疗可以与倔强的MPTC-NHL改进反应率和病人的2年的幸存率。进一步继续学习是必要的。
简介:目的评价无口咽侵犯的T1-2N0鼻咽癌不同设野放射治疗的近期疗效。方法将56例T1,2NoM01992年福州分期鼻咽癌病人随机分为面颈联合野组(A组)和面颈分野组(B组),A组先设面颈联合野和下颈切线野,均照射36Gy后改双耳前野24Gy,鼻前野8—10Gy和颈前切线野14Gy;B组设置双耳前野60Gy,鼻前野8-10Gy,颈前切线野50Gy。结果放疗结束时,鼻咽原发灶消退率A组和B组分别为96.4%和92.9%,两组无显著差异。A、B组2年总生存率分别为89.3%和85.7%。面颈分野组1例发生放射性颈脊髓损伤,两组急性和慢性放射损伤差异均无显著性。结论T1-2N0M0鼻咽癌病人选用面颈联合野或面颈分野,疗效无显著性差异。5年生存率及远期放射损伤情况有待进一步观察。
简介:背景与目的:脑胶质瘤是常见的颅内肿瘤,占脑恶性肿瘤的50%以上,化疗作为综合治疗的一项重要手段,仍存在效果不理想、易耐药等问题。本文就研究中华眼镜蛇毒组分C(FractionCfromNajaNajaActraVenom,NNAV.FC)体外对人胶质瘤细胞株U-251的细胞毒性作用,并与几种临床脑肿瘤化疗用药进行比较,以求为脑胶质瘤的治疗提供新的化疗药物。方法:应用MTT法.观察NNAV.FC人胶质细胞瘤株U-251的细胞毒作用、量效关系等,并与依托泊苷(vp=16、鬼臼乙叉苷、Vepesid)、卫萌(替尼泊苷、鬼臼甲叉甙vm-26,Teniposide)、顺铂(Cisplatin、CDDP)、卡铂(Carboplatin)、五氟尿嘧啶(5-FU)等对上述细胞的细胞毒作用进行比较。结果:FC对人胶质瘤细胞有明显的抑制作用,其24及48h的IC50分别为5.76和7.19μg/ml,且呈良好的量效关系。结论:在本实验中,FC能有效抑制胶质瘤细胞株的生长,抑制作用与浓度呈正相关,与临床胶质瘤化疗药相比较。FC是有效地抑制胶质瘤细胞生长的药物。