简介：Preparationofthetemporalboneforlightmicroscopyisanimportantstepinhistologicalstudiesoftheinnerear.Duetothecomplexityofstructuresoftheinnerear,itisdifficulttomeasureorcomparestructuresofinterestwithoutacommonlyacceptedstandardizedmeasureoftemporalbonesections.Therefore,standardizationoftemporalbonesectionsisveryimportantforhistologicalassessmentofsensoryhaircellsandperipheralganglionneuronsinthecochlearandvestibularsystems.Thestandardizedtemporalbonesectioningisorientedtoaplaneparalleltotheouterandinternalauditorycanals.Sectionsarecollectedfromtheepitympanumtothehypotympanumtoreveallayersintheorderofthecristaampullarisofthesuperiorandlateralsemicircularcanals,maculautriculiandmaculasacculi,superiorvestibularganglionneurons,maculaofsacculeandinferiorvestibularganglionneurons,cochlearmodiolus,endolymphaticductandendolymphaticsac,andfinallythecristaampullarisoftheposteriorsemicircularcanal.Moreover,technicaldetailsofpreparingfortemporalbonesectioningincludingfixation,decalcification,wholetemporalbonestaining,embeddingpenetration,andembeddingorientationarealsodiscussed.