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简介：这是一款功能强大的CD-copy程序。它工作于Raw模式，因此它能真实地1：1复制CD．不管是否有保护或加密之类．它仍然忠实地将它复制下来。到目前为止还没有一种软件能象CloneCD一样完整地复制CD。

简介：■ShanghaihasalwaysbeenChina'smostreceptivecity.Duringthenineteenthcentury,whiletherestofthecountryclungtoprovincialtraditions,Shanghaihadbeguntoactasagatewaytotherestoftheworld.Alongwiththeopiumfloodinginto

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简介：在上期杂志中，我们已经对首教DirectX10游戏进行了解析，并附上了目前主流显卡在《失落的星球》下的性能测试。本期的硬游地带中我们将继续关于DirectX10游戏的讨论，今天我们的主角是《英雄连》。微软希望消费者对DirectX10抱以希望图形芯片设计商也在半年前就拿出了DirectX10产品他们希望消费者能够在最短的时间内接受DirectX10产品。也许我们可以将他们的希望看作是一个笑话——现实具有相当的讽刺意味，数月以来，众多的游戏厂商只拿出了《失落的星球》，《狂野西部》以及《英雄连》三款基于DirectX10的游戏，而正式推出的只有《英雄连》一款游戏。

简介：目前。各校为了对教师进行量化绩效考核，加强了对教师教案的检查。这固然是一件好事，但这种教学常规管理上的“量化”要求。使备课工作追求形式，忽视内涵。这种貌似科学的量化管理使得教学被异化为精细操作的技术活动，教案变成了一堆可以描述、度量并能互相比较的数据。这种做法虽然方便了

简介：这篇文章提出一个新计划在延期高效地控制消息冗余性容忍的活动特定的网络(MANET)。网络的类通常缺乏端对端的连接。为了改善，发送消息的效率成功地被交付，多重消息拷贝路由协议通常被使用，但是网络负担由于很多消息冗余性被增加。在学习，由使用相反的方法，每个节点基于流行路由计划增加一个相遇柜台。柜台记录节点与一样的消息拷贝遇到另外的节点的数字。如果一个节点的柜台到达安装阀值，节点移开拷贝。理论分析在延期给阀值的更低的界限容忍的MANET。根据阀值的更低的界限，合理阀值在真实环境被安装。与建议计划消息，拷贝显然减少并且最后完全被移开。成功的交货效率仍然与流行路由一样，冗余的拷贝高效地被控制到一相对低级。计算机模拟在快、慢的活动性场面有关不同阀值给消息拷贝的变化。

简介：Arraycomparativegenomichybridization(CGH)hasbeenpopularlyusedforanalyzingDNAcopynumbervariationsindiseaseslikecancer.Inthisstudy,weinvestigated82sporadicsamplesfrom49breastcancerpatientsusing1-MbresolutionbacterialartificialchromosomeCGHarrays.Anumberofhighlyfrequentgenomicaberrationswerediscovered,whichmayactas'drivers'oftumorprogression.Meanwhile,thegenomicprofilesoffour'normal'breasttissuesamplestakenatleast2cmawayfromtheprimarytumorsiteswerealsofoundtohavesomegenomicaberrationsthatrecurredwithhighfrequencyintheprimarytumors,whichmayhaveimportantimplicationsforclinicaltherapy.Additionally,weperformedclasscomparisonandclasspredictionforvariousclinicopathologicalparameters,andalistofcharacteristicgenomicaberrationsassociatedwithdifferentclinicopathologicalphenotypeswascompiled.Ourstudyprovidescluesforfurtherinvestigationsoftheunderlyingmechanismsofbreastcarcinogenesis.

简介：ExtensiveexcavationsatthesiteofAshurallowedOlafPederséntoreconstruct,toaconsiderablelevelofdetail,themanyarchivesandlibrariesinofficialandprivatecollectionsthatexistedatancientAshurthroughthemiddle

简介：Amolecularmodelofpancreaticzymogengranule（ZG）iscriticalforunderstandingitsfunctions.WehaveextensivelycharacterizedthecompositionandmembranetopologyofratZGproteins.Inthisstudy,wereportthedevelopmentoftargetedproteomicsapproachestoquantifyrepresentativemouseandhumanZGproteinsusingLC-SRMandheavyisotope-labeledsyntheticpeptides.TheabsolutequantitiesofmouseRab3DandVAMP8weredeterminedas1242±218and2039±151（mean±SEM）copiesperZG.ThesizedistributionandtheaverageddiameterofZGs750±23nm（mean±SEM）weredeterminedbyatomicforcemicroscopy.TheabsolutequantificationofRab3Dwasthenvalidatedusingsemi-quantitativeWesternblottingwithpurifiedGST-Rab3Dproteinsasaninternalstandard.Toextendourproteomicsanalysistohumanpancreas,ZGswerepurifiedusinghumanaciniobtainedfrompancreaticislettrans-plantationcenter.OnehundredandeightyhumanZGproteinswereidentifiedforthefirsttimeincludingboththemembraneandthecontentproteins.Furthermore,thecopynumberperZGofhumanRab3DandVAMP8weredeterminedtobe1182±45and485±15（mean±SEM）.ThecomprehensiveproteomicanalysesofmouseandhumanpancreaticZGshavethepotentialtoidentifyspecies-specificZGproteins.ThedeterminationofproteincopynumbersonpancreaticZGsrepresentsasignificantadvancetowardsbuildingaquantitativemolecularmodelofaprototypicalsecretoryvesicleusingtargetedproteomicsapproaches.TheidentificationofhumanZGproteinslaysafoundationforsubsequentstudiesofalteredZGcompositionsandsecretioninpancreaticdiseases.

简介：使用过DOS的朋友差不多都使用过磁盘对拷程序HD-COPY。HD-COPY功能非常强大，可以用作格式化软盘，给软盘扩容，修复坏软盘，读取坏软盘等，从92年问世至今一直深受们们的喜爱。今天笔者介绍一款国产的磁盘综合工具—FDC，它在功能上不仅超越了HD-COPY，并且还有自己的独特之处。

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简介：AbstractBackground:Hematopoietic stem cells (HSCs) have the ability to differentiate into all subsets of blood cells and self-renew. Large tumor suppressor 1 (LATS1) and large tumor suppressor 2 (LATS2) kinases are essential for cell cycle regulation, organism fitness, genome integrity, and cancer prevention. Here, we investigated whether Lats1 and Lats2 are critical for the maintenance of the self-renewal and quiescence capacities of HSCs in mice.Methods:Quantitative reverse transcription-polymerase chain reaction was used to determine the expression levels of Lats1 and Lats2 in subsets of progenitor cells and mature bone marrow cells. A clustered regularly interspaced short palindromic repeats system was used to generate Lats1 or Lats2 knockout mice. Complete blood cell counts were used to compare the absolute number of white blood cells, lymphocytes, monocytes, neutrophils, and platelets between Lats1 or Lats2 heterozygotes and littermates. Flow cytometry was used to assess the size of hematopoietic progenitor cells (HPCs) and HSC pools in Lats1 or Lats2 heterozygotes and littermates. The comparison between the two groups was analyzed using Student’s t test.Results:Lats1 and Lats2 were widely expressed in hematopoietic cells with higher expression levels in primitive hematopoietic cells than in mature cells. Lats1 or Lats2 knockout mice were generated, with the homozygotes showing embryonic lethality. The size of the HPC and HSC pools in Lats1 (HPC: wild-type [WT] vs. heterozygote, 220,426.77 ± 54,384.796 vs. 221,149.4 ± 42,688.29, P = 0.988; HSC: WT vs. heterozygote, 2498.932 ± 347.856 vs. 3249.763 ± 370.412, P = 0.105) or Lats2 (HPC: WT vs. heterozygote, 425,540.52 ± 99,721.86 vs. 467,127.8 ± 89,574.48, P = 0.527; HSC: WT vs. heterozygote, 4760.545 ± 1518.01 vs. 5327.437 ± 873.297, P = 0.502) heterozygotes were not impaired. Moreover, the depletion of Lats1 or Lats2 did not affect the overall survival of the heterozygotes (Lats1: P = 0.654; Lats2: P = 0.152).Conclusion:These results indicate that a single allele of Lats1 or Lats2 may be sufficient for normal hematopoiesis.

简介：如果你注册了iTunesMatch服务．你可以把你老的音乐文件替换成更棒的256-kbpsAAC的版本。

简介：许多凤凰同仁苦恼节目总是被人COPY了，我说这是好事，我们不用怕被别人模仿，创造力谁也学不去。不断推陈出新，锻造品牌栏目，引领电视潮流向上向前，也成了凤凰另一个风格。

简介：【摘要】目的：分析低病毒载入量人类免疫缺陷病毒（HIV）患者耐药性，为临床低病毒载入量HIV患者治疗提供参考。方法：选择2022年1月-2023年1月在接受治疗的病毒载入量＜1000copy/ml 的67例HIV患者作为研究对象，所有患者开展均需开展HIV病毒载量检测，采用in-house的方法开展HIV-1基因型耐药检测，并使用专业软件进行序列拼接，最终对比分析，完成耐药序列比对和基因亚型分析使用。结果：67例低病毒载入量HIV患者中获取12份序列，其中6（8.96%）例产生不同耐药性，CRF07-BC亚型共8（66.67%）例，B亚型2（1.67%）例，CRF01-AE亚型1（8.33%）例。结论：需要及时开展低病毒载入量HIV患者耐药检测，以更改与调整治疗方案，从而确保治疗效果

简介：Objective:Theepidermalgrowthfactorreceptor(EGFR)inhibitorsmonoclonalantibodies(MoAbs)havealreadyshownthetherapeuticeffectivenessinpatientswithmetastaticcolorectalcancer(mCRC).Butmanypatientsresisttothetreatment.Theaimofthismeta-analysiswastoassessEGFRgenecopynumber(GCN)asacandidatepredictivebiomarkerforresistancetoanti-EGFRMoAbsinmCRCtreatment.Methods:SystematiccomputerizedsearchesofthePubMed,EMBaseandCochraneLibrarywereperformed.Theprimaryendpointwasobjectiveresponserate(ORR).Thesecondendpointsincludedprogression-freesurvival(PFS),andoverallsurvival(OS).Thepooledoddratio(OR)andpooledsensitivity,specificity,andsummaryreceiveroperatorcharacteristic(SROC)forORRwereestimated.Thepooledhazardratios(HR)forPFSandOSwerealsocalculated.Results:Fourteenstudieswith1,021patientswereincluded.IncreasedEGFRGCNwasassociatedwithincreasedORR(OR=6.905;95%CI:4.489-10.620).Itwasalsofoundinwild-typeKRASmCRCpatients,withthepooledORof8.133(95%CI:4.316-15.326).GCNhasmediumvalueforpredictingORR,withthepooledsensitivityof0.79(95%CI:0.73-0.84),thepooledspecificityof0.59(95%CI:0.55-0.62).InwildtypeKRASmCRCpatients,thesensitivityandthespecificitywere0.80(95%CI:0.70-0.87)and0.60(95%CI:0.53-0.66),respectively.IncreasedEGFRGCNwasassociatedwithincreasedPFS(HR=0.557;95%CI:0.382-0.732)andOS(HR=0.579;95%CI:0.422-0.737).Conclusions:Thismeta-analysissuggeststhatEGFRGCNrepresentsapredictivebiomarkerfortumorresponseinmCRCpatientstreatedwithMoAbsregardlessofKRASmutation.mCRCpatientswithincreasedEGFRGCNaremorelikelytohaveabetterresponse,PFS,andOSwhentreatedwithcetuximaborpanitumumab.

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