简介：目的：探讨中老年屈光异常性视疲劳与干眼症的联系,以及戴镜屈光矫正对干眼症治疗疗效的影响。方法：按照统一标准,门诊随机调查视疲劳的患者,分析其中屈光不正性视疲劳的患病率。在视疲劳的患者中调查干眼症的发生情况,分析屈光不正性视疲劳与干眼症的联系。再把干眼症合并屈光异常性视疲劳患者随机分为戴镜治疗、药物治疗和对照组,治疗3mo,观察干眼症的治疗效果。结果：40岁以上视疲劳的患者中因屈光不正而引起的占42.0%。屈光不正性视疲劳与干眼症存在显著联系（P〈0.05）。随访3mo,戴镜治疗组干眼症状好转明显高于药物治疗组（P〈0.05）。结论：中老年屈光异常性视疲劳与干眼症存在紧密联系,戴镜矫正治疗屈光异常性视疲劳可以明显改善干眼症状。

简介：目的：研究近视患者LASIK术后视疲劳的发生与融合范围的关系，及同视机融合功能训练改善LASIK术后视疲劳的疗效观察。方法：对60例120眼近视患者（．1．50--8．00D）施行LASIK手术，于术前戴全矫眼镜用同视机法测定融合范围，并进行视疲劳的问卷调查。术后将其随机平分为两组：A组进行同视机融合功能训练30d，B组不进行同视机融合功能训练，分别对其术后1wk；1mo行同视机法测定融合范围，并进行视疲劳的问卷调查及统计学分析。结果：A、B组术后融合范围均较术前减小。术后1wk时融合范围两组分别为18．58±8．91和13．45±8．87，二者差异有统计学意义（P〈0．05）。术后1mo时，两组融合范围分别为20．55±7．23和18．12±6．10，二者差异无统计学意义。术后1wk时A组视疲劳评分3．92±1．65，B组评分5．16±2．34，二者差异有统计学意义（P〈0．05）。术后1mo时两组评分分别是1．28±0．96和1．45±0．99分，二者差异无统计学意义。结论：近视眼LASIK术后双眼的融合功能下降是出现视疲劳的一个重要因素。术后早期进行同视机融合功能训练可以有效的改善视疲劳症状。

简介：目的：评价七叶洋地黄双苷滴眼液治疗近视患者准分子激光原位角膜磨镶术（1aseffnsitukeratomileusis，LASIK）后视疲劳的临床疗效。方法：对200例400眼近视患者行LASIK手术，随机分成两组。100例为试验组应用七叶洋地黄双苷滴眼液，另100例为对照组应用潇莱威滴眼液。分别于手术前、用药后1，2，4wk评估患者术后视疲劳的症状和体征，同时观察药物不良反应。结果：随着术后时间的延长，试验组和对照组近视患者视疲劳症状逐渐缓解，在术后1，2wk时，试验组的视疲劳评分低于对照组，两组差异有统计学意义（t=3．160，P=0．001；t=2．727，P=0．0025）；术后4wk时，两组的差异无统计学意义（t=1．423，P=0．10）。结论：随着观察时间的延长，LASIK术后患者视疲劳症状可逐渐得到恢复和改善，七叶洋地黄双苷滴眼液对改善LASIK术后早期视疲劳症状有效。

简介：目的观察七叶洋地黄双苷滴眼液（施图伦）对青少年低度数近视及视疲劳症状的治疗效果。方法收集于2015年6月至9月就诊于浙江省台州医院眼科门诊的青少年近视患者120例240眼,根据用药情况不同随机分为对照组,托吡卡胺组,施图伦组,每组40例80眼,分别比较三组用药前与用药后一月的裸眼视力与屈光状态,根据视疲劳问卷调查表评估用药前后的视疲劳症状变化。结果（1）裸眼视力：施图伦组及托吡卡胺组较治疗前裸眼视力均有一定程度提高,差异有统计学意义（P〈0.05）。（2）近视度数：施图伦组较治疗前矫正度数下降,差异有统计学意义（P〈0.05）,托吡卡胺组矫正度数较治疗前有下降,差异有统计学意义（P〈0.05）。对照组矫正度数较治疗前对比无差异。（3）视疲劳症状：施图伦组视疲劳症状改善37例,总改善率达92.5%。对照组及托吡卡胺组对比治疗前差异无统计学意义。结论施图伦滴眼液能显著改善近视患者的视疲劳症状,对于低度数近视有一定的治疗作用。

简介：青光眼是全球第二大致盲眼病,是不可逆性盲最主要原因,而原发性闭角型青光眼（primaryangleclosure[1]glaucoma,PACG）是我国最主要的青光眼类型。原发性闭角型青光眼（PACG）的发病机制复杂。PACG发病的主要危险因素是晶状体位置偏前、前房变浅而导致的瞳孔阻滞。此外,周边虹膜肥厚以及睫状体位置前移也是PACG发病的常见危险因素。

简介：目的分析常年性变应性鼻炎的狗、猫上皮变应原皮肤点刺试验的阳性结果，对其他气传变应原的反应性，并进一步探讨环境接触与其相关性。方法总结1022例常年性变应性鼻炎患者采用阿罗格叶7种气传变应原标准化试液进行的皮肤点刺试验；分析狗、猫上皮变应原的皮试结果及合并其他气传变应原阳性的比例；并根据是否有环境接触分类，比较各组在阳性率分布上的差异。结果常年性变应性鼻炎中狗上皮变应原皮试阳性占13．80％，猫上皮阳性占6．95％；且98％以上合并其他气传变应原阳性，尤以合并粉尘螨、屋尘螨阳性居多。其中21．93％和26．32％环境接触狗／猫的变应性鼻炎相应狗／猫上皮变应原皮试阳性；无环境接触组的阳性率分别为12．78％和6．58％，差异均有统计学意义（P〈0．01）。结论常年性变应性鼻炎的狗上皮及猫上皮的阳性率位居前列，其中绝大多数合并其他气传变应原阳性。鉴于环境接触对狗、猫上皮过敏的高危险性，变应性鼻炎患者应该尽可能地避免饲养、接触宠物。

简介：AIM:TotrainTibetanmonkey(Macacathibetana)forintraocularpressure(IOP)measurementinconsciousstateandobtainnormalIOPinconsciousTibetanMacaque.·METHODS:Thetrainingwasbasedonaward-conditionedbehavior.Foodstimulationandhuman-animalinteractionwereusedinthistraining.·RESULTS:TrainedTibetanmonkeyscalmlyacceptedIOPmeasurementbytheTonoVet?誖reboundtonometerwithoutsedationoranesthesiaandtheirIOPvaluesweresimilartootherprimates.·CONCLUSION:Human-cultivatedThibetanmonkeysaretamable,andcanbeusedforbiomedicalresearchsuchasophthalmicresearchwithoutanesthesia.

简介：AIM:ToevaluatetheefficacyofSeprafilmtransplantationfollowingadhesiolysisforpreventingpostoperativereadhesionandimprovingsurgicaloutcomes.METHODS:Primaryblepharoplastywascarriedoutonbotheyelidsof18albinorabbits.After2weeks,anewskinincisionwasmade,andadhesiolysiswasperformedonbotheyelids.Therabbitswerecategorizedintotwogroups,onewithadhesiolysisaloneinthelefteyelid(controlgroup),andtheotherwithadhesiolysiswithaSeprafilmgraftintherighteyelid(Seprafilmgroup).Thedegreesofinflammationandfibrosiswereexaminedwithhematoxylin-eosin(HE)andMasson’strichromestains.Expressionofα-smoothmuscleactin(α-SMA)wasalsoimmunohistochemicallyexamined.RESULTS:Eyelidexaminationimmediatelyaftertheoperationrevealedmildswellingandhemorrhageinbothgroups,butthesesymptomsresolvedafter1week-2weeks,andeyelidshapehadrecoveredcompletelyinbothgroups.MicroscopicassessmentsdemonstratedthattheSeprafilmgroupshowedlessinflammationandfibrosisthanthecontrolgroup.TheSeprafilmgroupalsoexhibitedfewerα-SMA-positivecellsthanthecontrolgroup.CONCLUSION:Basedonthesefindings,weconcludethatSeprafilmgraftwithadhesiolysisisaneffectivemethodforpreventingpostoperativereadhesionsaftereyelidsurgery.

简介：·AIM:Toinvestigatethetherapeuticeffectsofnanophtha-locyaninephotosensitizersonanexperimentalratchoroidalneovescularization(CNV)model,aswellastoevaluatethecytotoxicityofwhichonhumanretinalpigmentepithelia(HRPE)andhumanretinalendothelialcells(HRECs).·METHODS:Twotypesofphotosensitizers,G1-ZnPc(COOH)8andG1-ZnPc(COOH)8/mrespectively,wereadministratedforphotodynamictherapy(PDT)afterasuccessfulestablishmentofCNVmodelonBrown-Norway(BN)ratsviafundusphotocoagulation.Thetherapeuticeffectsofthetwodrugswereassessedthroughopticalcoherencetomography(OCT),fluoresceinfundusangiography(FFA)andtransmissionelectronmicroscopy(TEM).Forcytotoxicitytests,cellcountingkit-8(CCK-8)assaysandchangesofmitochondrialtransmembranepotential(△Ψm)wereconductedonHRPEandHRECsafterinitialuptakeofthetwodrugs.·RESULTS:Bothphotosensitizersdemonstratedanimprove-mentofvascularleakageandclosureofCNV1weekafterPDTasconfirmedbyfundusimage,OCT,FFAandTEM.TwoweeksafterPDT,G1-ZnPc(COOH)8/mshowedabetterCNVclosureeffectversusG1-ZnPc(COOH)8(P<0.05).Asignificantdifference(P<0.01)wasfoundinuptakeofthetwodrugsinHRPEandHRECs,withnodifferencebetweenthedrugs(P>0.05).BothphotosensitizersshowedcytotoxicityonHRPE,butG1-ZnPc(COOH)8/minducedalowercellviability.·CONCLUSION:G1-ZnPc(COOH)8/mmediatedPDTisbetterthanG1-ZnPc(COOH)8inCNVclosureandalsohavetheadvantageoffastmetabolismleadingtolesssideeffect.·

简介：AIM:ToevaluatetheeffectofCollagencross-linkingonthepreventionofmeltinginrabbitcorneasafteralkaliburn.·METHODS:TwentyNewZealandwhiterabbitswererandomlydividedintomodelcontrolgroupandcollagencross-linkingtreatmentgroup.Thesecondgroupofrabbitsreceivedcollagencrosslinkedtreatment.Bothgroupswereappliedwithantibioticeyedropstopreventinfection.Thecorneaswereevaluatedformelting,opacity,pathologicalandimmunohistochemistry,recordthechangeswhen28daysaftertheanimalswerekilled.·RESULTS:Inthecontrolgroup,6outof8rabbitsshowedcornealmeltingafterinjury(14±4)days,whiletwocornealperforated.Incollagencross-linkingtreatmentgroup,onerabbitshowedcornealmeltingafterinjury23days,withoutcornealperforation;cornealdissolutionratebetweenthetwogroupswassignificantlydifferent(P<0.05).Pathologicalexaminationsuggestedthatinthetreatmentgroup,mildcornealedema,milddamagetocollagenfibers,inflammatorycellinfiltrationwassignificantlylessthanthecontrolgroup.Immunohistochemistryshowedthatcornealcollagenfibersarrangedinneatrowsinthecontrolgroup.·CONCLUSION:Collagencross-linkingtreatmentnotonlycanpreventanddelaythecornealmeltingafteralkaliburn,butalsocanreducethedestructionofcornealcollagenfibersandinfiltrationofinflammatorycellsinthecornealtissue.

简介：·AIM:Toexploretheeffectofimmunizationwithcopolymer-1(COP-1)andretinalstemcells(RSCs)transplantationoninterferon-gamma(IFN-γ)levelsinaratexperimentalglaucomamodel.·METHODS:Anexperimentalglaucomawasinducedbyargonlaserphotocoagulationoftheepiscleralveinsandlimbalplexusintherighteyeofrats.Immediatelyfollowingglaucomainduction,ratswereimmunizedwithCOP-1.RSCswereculturedandtransplantedintravitreallyintotheeyesofglaucomamodelanimals1weekpost-lasertreatment.Sixexperimentalgroupswereused:COP-1/RSC,PBS/RSC,COP-1/PBS,PBS/PBS,glaucomamodelgroup,andanormalcontrolgroup.TheconcentrationofIFN-γinaqueoushumor(AH)andserumwasmeasuredbyenzyme-linkedimmunosorbentassay(ELISA)ineachofthesixgroups.Retinalganglioncell(RGC)survivalwasassessedbyquantifyingapoptosisusingHoechststaining.·RESULTS:ConcentrationsofIFN-γinAHandserumofratsthathadundergoneglaucomainductionwerehigherthanthoseofnon-inducedcontrolrats.TheconcentrationsofIFN-γinAHandserumoftheCOP-1/RSCstreatedgroupweredeterminedtobe2371.9ng/Land710.9ng/L,respectively,whichweresignificantlylowerthanthoseintheothertreatedgroups(P<0.05).Infact,IFN-γlevelsinthedualtreatedgroupwerereducedtobackgroundlevels.TheCOP-1/RSCgrouphadlowernumberofapoptoticRGCsthantheotherthreeexperimentalgroups(P<0.05).·CONCLUSION:ThereducedlevelsofIFN-γinAHandserumoftheCOP-1/RSCgroupmayberelatedtosynergisticeffectsbetweenRSCstransplantationandCOP-1immunemodulation.ItislikelythatthelowerlevelsofIFN-γpreventedRGCsglaucomatousapoptosis.·