简介：AbstractObjective:In order to study the important role and molecular mechanism of Brevinin-2 family antimicrobial peptide Brevinin-2ISb in methicillin-resistant Staphylococcus aureus (MRSA) infection of Caenorhabditis (C.) elegans, and to find the optimal therapeutic concentration of Brevinin-2ISb.Methods:By using a C. elegans model and MRSA infection modelto study the therapeutic effect of different concentrations of Brevinin-2ISb on C. elegans. Real-time PCR was used for investigating the effect of Brevinin-2ISb on the downstream gene expression of DAF-2/DAF-16 innate immune pathway and the major virulence factor gene expression of MRSA. With protein activity tests to study the inhibitory effect of Brevinin-2ISb on MRSA virulence factor protein activity. Finally, laser confocal imaging was carried out to observe real-time expression and distribution of downstream antimicrobial proteins to further prove the effect of Brevinin-2ISb on the activation of DAF-2/DAF-16 pathway by in vivo imaging. All animal study procedures were approved by the Academic Committee at Xidian University and Xi’an Jiaotong University Animal Care and Use Committee, China (approval No. JGC201207) on July 15, 2017.Results:Host immunity was largely enhanced by Brevinin-2ISb, and the expression of staphylococcal enterotoxin genes, as well as virulence factors, was suppressed by Brevinin-2ISb. Indeed, the expression of many C. elegans innate immune genes, including lys-7, spp-1, K05D8.5 and C29F3.7, was induced by Brevinin-2ISb. In particular, robust, sustained expression of the antibacterial gene lys-7 was observed after Brevinin-2ISb treatment, resulting in increased protein levels. These effects correlated with a reduction in the MRSA-mediated death of the C. elegans host. Low concentrations of Brevinin-2ISb exhibited very low hemolytic activity, and may play a positive role in host innate immunity. Specifically, activation of the DAF-2/DAF-16 pathway appears to be essential for immune activation in C. elegans treated with Brevinin-2ISb. Based on the evolutionary conservation of innate immune pathways, our results suggest that Brevinin-2ISb not only has strong antibacterial activity, but may also enhance the innate immune response in humans. This study demonstrates that Brevinin-2ISb-related peptides are potential candidates for the development of novel anti-inflammatory or anti-microbial drugs.Conclusion:Antimicrobial peptide Brevinin-2ISb effectively inhibits MRSA at low concentration. This antimicrobial peptide can prolong the life of MRSA-infected C. elegans, has very low hemolytic activity and inhibits the activity and expression of various MRSA virulence factors. More importantly, Brevinin-2ISb activated the expression of antimicrobial genes downstream of DAF-2/DAF-16, which enhanced the MRSA resistance of C. elegans. This peptide could be used as the basis for developing new drugs to replace antibiotics.

简介：摘要：场站保障能力评估是摸清场站能力底数、检验问题不足、提升保障能力的重要途径。本文通过建立场站保障能力评估指标体系，构建DAF评估模型并以外军为例进行验证，以期为场站军事训练和保障能力提升提供有益的参考价值。

简介：弗洛伊德曾这样评价达芬奇：“当其他人还在沉睡的时候，达芬奇已经在一片黑暗中醒来：”达·芬奇作品中科学和艺术的完美结合、几百年来一直吸引着世人的目光。而最近《达·芬奇密码》的畅销更让这个天才显得神秘莫测、那幺真实的达·芬奇是什幺样子呢?

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简介：摘要CO2灭火系统是燃机安全运行的重要保护系统，系统复杂，本文对燃机CO2灭火系统的配置和特点进行了分析和总结，同时也介绍了CO2灭火系统运行中应注意的问题。

简介：Ｏｂｗｏｈｌ，ｔｒｏｔｚｄｅｍ，ｄａｕｎｄｄｅｓｗｅｇｅｎＢｅｒｎｔＥｎｇｅｌｍａｎｎＯｂｗｏｈｌ’ｓｓｏｌｅｉｃｈｔ，ｔｒｏｔｚｄｅｍｗｉｒｄ’ｓｆａｌｓｃｈｇｅｍａｃｈｔ！ＷｅｒｎｉｅｄｅｎＵｎｔｅｒｓｃｈｉｅｄｂｅｄａｃｈｔ，ｄｅｒｚｗｉｓｃｈ...

简介：档案图书的卫士——DA91多效杀虫灵、DA99特效防虫灵是重庆赛新档案科技开发研究所与重庆种衣剂厂、四川日用化工研究所联合研制生产的档案杀虫药剂。DA91多效杀虫灵是从多种植物提取的纯天然精油研制而成，是代替化学防虫剂最理想的新型防虫药剂，对

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简介：摘 要：随着经济的快速发展和生活质量的不断提高，人们对电力供应的需求日益增长，保证电力连续可靠供应成为电力企业的重要工作目标。变电站作为连接主网和配网的中间点，站内设备是否安全稳定运行与用户的用电质量有着紧密的联系，然而，变电站大多都分布在郊区，饱受鸟害的困扰，给电网设备的安全运行带来威胁，对于防治变电站鸟害的问题，我们也一直在研究，寻找一种可行、经济、有效的解决措施，本文针对 110kV阿海珐水平旋转式刀闸（ S2DA2T）鸟害的问题进行分析、探讨。

简介：该项目是由Riveira市主导建造的，用以鼓励当地旅游业的发展。瞭望台自19世纪80年代开始便已投入使用。人工搭建的混凝土台阶让游客可以驻足石上，饱览大西洋的绝美景致。

简介：本文总结了VCM装置EDC脱水塔和脱轻组分塔、VCM精馏塔塔板的改造情况．对比了改造前后塔的运行参数。取得了明显的效果．满足了扩产需要。

简介：本文验证了恒星目视表面亮度参数Fv与色指数（V－I）之间的单值关系并将其应用于白矮星半径测定研究，对68颗白矮星求得了平均线半径为0.0103R⊙；在对DA型和非DA型白矮星的关分别进行考察中发现：平均来说，非DA型白矮星的线半径比DA型白矮星小12％左右。

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简介：2018年11月12日,全球工业自动化与网络领域通信、监测和控制专家美国红狮控制公司,宣布推出DA10D和DA30D协议转换和数据采集设备。不同于其它依赖复杂脚本或需要通过其他服务器硬件连接到企业系统的网关或协议转换器,DA10D和DA30D具有功能强大的连接选项和简单易用的配置环境,方便企业客户快速解锁孤立或老式设备中的高价值数据,从而经济高效地连接、监测和管理各类系统。

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简介：通过对DA阀的控制原理进行系统分析,论证了其利用DA阀的自动调节作用,可以始终保持液压泵扭矩与发动机的输出扭矩的最佳匹配,防止了机械系统发动机因负载过大而熄火,并提高了使用效率,同时也减轻操作者的心理负担。DA阀在机械行业必将得到越来越广泛应用。

简介：Lowercontentofcarboncanfurtherimprovethestressrupturelifeofp-modifiedDA718alloyuptomorethan270%.Meanwhile,theductilityofthealloydecreasedalittle.Moreboronatomsdissociateduetodecreasingcarboncontentandinteractwithphosphoruswhichbringsthelongerstressrupturelifeofthealloy.Lesscarbonmayinducemorephosphorussegregatinginthegrainboundaryandresultinbrittleness.

简介：介绍DA350-61空压机的性能和结构,分析空气量不足的主要原因是中间冷却器效果不好引起的.采取措施的同时结合增大排气量,对叶轮的宽度进行加宽处理,达到了良好的效果.