简介:AbstractBackground:The Nuclear Dbf2-related (NDR1) kinase is a member of the NDR/LATS family, which was a supplementary of Hippo pathway. However, whether NDR1 could inhibit glioblastoma (GBM) growth by phosphorylating Yes-associated protein (YAP) remains unknown. Meanwhile, the role of NDR1 in GBM was not clear. This study aimed to investigate the role of NDR1-YAP pathway in GBM.Methods:Bioinformation analysis and immunohistochemistry (IHC) were performed to identify the expression of NDR1 in GBM. The effect of NDR1 on cell proliferation and cell cycle was analyzed utilizing CCK-8, clone formation, immunofluorescence and flow cytometry, respectively. In addition, the xenograft tumor model was established as well. Protein interaction was examined by Co-immunoprecipitation and immunofluorescence to observe co-localization.Results:Bioinformation analysis and IHC of our patients’ tumor tissues showed that expression of NDR1 in tumor tissue was relatively lower than that in normal tissues and was positively related to a lower survival rate. NDR1 could markedly reduce the proliferation and colony formation of U87 and U251. Furthermore, the results of flow cytometry showed that NDR1 led to cell cycle arrest at the G1 phase. Tumor growth was also inhibited in xenograft nude mouse models in NDR1-overexpression group. Western blotting and immunofluorescence showed that NDR1 could integrate with and phosphorylate YAP at S127 site. Meanwhile, NDR1 could mediate apoptosis process.Conclusion:In summary, our findings point out that NDR1 functions as a tumor suppressor in GBM. NDR1 is identified as a novel regulator of YAP, which gives us an in-depth comprehension of the Hippo signaling pathway.
简介:摘要:机械结构类申请的改进点一般在常规的机械结构之间的配合,从技术手段的角度出发难以扩展关键词。本文根据实际检索经验,提出从技术效果的角度出发进行检索,并给出了effect字段在技术效果检索中的应用,实现机械结构类申请的快速检索。
简介:目的研究网页挂马案件的检验方法,为有效打击此类犯罪,维护信息网络安全提供技术支持。方法在染毒主机端重新浏览可疑网页,使用wireshark捕获通信数据,借助wireshark提供的文件还原功能从通信数据中还原出所有文件,再利用主流杀毒引擎对这些文件进行病毒扫描,记录下木马程序的名称和下载路径。之后利用本文的Referer字段递归分析方法还原木马完整的种植过程,找出被挂马网站和相应的页面,提取出网页中被植入的恶意代码。结果获得了被挂马网站的相关信息,准确地定位出恶意代码的存储位置,提示网站管理员除去恶意代码并增强保护措施,从源头阻断木马的传播途径。根据黑客种植恶意代码过程中在被挂马网站遗留的入侵痕迹,对挂马网站进行检验分析,获得黑客的相关线索。结论应用本文提出的Referer字段递归分析方法可以有效检验网页挂马类案件,获取相关线索。
简介:[摘要]通过对数字图书馆元数据和对象数据的介绍,提出了856字段在其中所起的作用,并对856字段的应用、实现方式作了详细的介绍。
简介:摘 要:智能化检索系统保留并优化了S系统现有功能,新增了智能检索功能,并对文献数据库资源进行整合。本文结合高分子领域的几个实际案例,将智能化检索系统检索过程与S系统界面检索进行了对比,探讨了智能化检索系统中新增特殊字段及同在算符标引规则对高分子领域中全文库检索效能的影响。