简介：AbstractObjective:To investigate the expressions of MAPK10, c-Jun and Itga6 in laryngeal carcinoma and its influence on the sensitivity to docetaxel, cisplatin and 5-fluorouracil (TPF) chemotherapy.Methods:Fifty-seven patients with supraglottic squamous cell carcinoma, who were treated by two cycles of TPF induction chemotherapy in our hospital, were enrolled in this study and divided into groups by chemotherapy resistance or chemotherapy sensitivity. The expressions of mRNA and protein of MAPK10, c-Jun and Itga6 in tumor tissues were evaluated by immunohistochemistry. The consistency of mRNA and protein expressions was tested, and the relation with the clinicopathological features was analyzed.Results:The positive rates of MAPK10 andc-Jun in the tumor tissues of the sensitive group were significantly higher than those of there assistant group, which was 90.48% and 100.00%, respectively. The expression rate of Itga6 was significantly higher in the resistant group, which was 83.33% (P < 0.05). The mRNA levels of MAPK10 and c-Jun were significantly lower in the resistant group than in the sensitive group, whilethemRNA levelof Itga6was significantly higher in the resistant group (P < 0.05). The protein expressions of MAPK10, c-Jun and Itga6 were consistent with their mRNA expressions (P < 0.05). The expressions of MAPK10, c-Jun and Itga6 were not correlatedwithage, gender and tumor diameter (P > 0.05). However, the expressions of MAPK10 and c-Jun were negatively correlated withclinical stage and pathological grading (P < 0.05). Negative correlations between MAPK 10 and Itga6, and between c-Jun and Itga6in tumor tissues were found by Spearman’srank correlation coefficient (P < 0.05). The correlation was also negative in the resistant tumor tissues (P < 0.05).Conclusion:The MAPK10 and c-Jun expressions were down-regulated, while the Itga6 expression was up-regulated in the chemo-resistant laryngeal carcinoma, and the expression levels of different factors were correlated witheach other. These factorsmight be important biomarkers for predicting outcomes of TPF chemotherapy in laryngeal carcinoma in the future.

简介：Laryngealsquamouscellcarcinoma(LSCC)remainsahighlymorbidandfataldisease.Historically,ithasbeenamodelexamplefororganpreservationandtreatmentstratificationparadigms.Unfortunately,survivalforLSCChasstagnatedoverthepastfewdecades.Astheeraofnext-generationsequencingandpersonalizedtreatmentforcancerapproaches,LSCCmaybeanidealdiseaseforconsiderationoffurthertreatmentstratificationandpersonalization.Here,wewilldiscusstheimportanthistoryofLSCCasamodelsystemfororganpreservation,uniqueandpotentiallytargetablegeneticsignaturesofLSCC,andmethodsforbringingstratified,personalizedtreatmentstrategiestothe21~(st)century.

简介：Objective:ToexploretherelationshipbetweenSTK15geneabnormalexpressionandlaryngealcarcinoma.Methods:Tumortissuesandmatchednormaltissuesweretakenfrom55LSCCpatients.Semi-quantitativereversetranscription-polymerasechainreaction(RT-PCR)wasusedtodetectSTK15expressionin110specimens.Results:In38ofthe55cases(69.1%),theSTK15expressionatthemRNAlevelswashigherthanthatofthepairednormaltissue.TheratioofADV(averagedensityvalue)ofSTK15genetoADVofβ-actingenewas1.22±0.49inthecancertissue,and0.99±0.54inthepairednormaltissuewithasignificantdifference(t=4.539,P<0.01).Conclusion:TherewasobviousassociationbetweentheSTK15overexpressionandlaryngealcarcinoma.Itmayserveasanalternativemechanismofactivatingthepathogenesisofhumanlaryngealsquamouscellcarcinoma.

简介：Todeterminethevalueofdissectingtherecurrentlaryngealnerveduringthyroidsurgerywithrespecttopreventingrecurrentlaryngealnerveinjury,weretrospectivelyanalyzedclinicaldatafrom5344patientsundergoingthyroidectomy.Amongthesecases,548underwentdissectionoftherecurrentlaryngealnerve,while4796didnot.Therewere12casesofrecurrentlaryngealnerveinjuryfollowingrecurrentlaryngealnervedissection(injuryrateof2.2%)and512casesofrecurrentlaryngealnerveinjuryinthosenotundergoingnervedissection(injuryrateof10.7%).Thisdifferenceremainedstatisticallysignificantbetweenthetwogroupsintermsoftypeofthyroiddisease,typeofsurgery,andnumberofsurgeries.Amongthe548casesundergoingrecurrentlaryngealnervedissection,128developedanatomicalvariationsoftherecurrentlaryngealnerve(incidencerateof23.4%),butnorecurrentlaryngealnerveinjurywasfound.Inaddition,theincidenceofrecurrentlaryngealnerveinjurywassignificantlylowerinpatientswiththeinferiorparathyroidglandandmiddlethyroidveinsusedaslandmarksforlocatingtherecurrentlaryngealnervecomparedwiththosewiththeentryoftherecurrentlaryngealnerveintothelarynxasalandmark.Thesefindingsindicatethatanatomicalvariationsoftherecurrentlaryngealnervearecommon,andthatdissectingtherecurrentlaryngealnerveduringthyroidsurgeryisaneffectivemeansofpreventingnerveinjury.

简介：Objective:Patientswithheadandneckcanceroftensufferfrommalnutrition.Thisstudyaimstoinvestigatetheinfluenceofbodymassindex(BMI)ontheprognosisoflaryngealsquamouscellcarcinoma(LSCC).Methods:Atotalof473patientswithLSCCinitiallytreatedatSunYat-senUniversityCancerCenterbetweenJanuary2005andJuly2009wereretrospectivelyreviewed.SurvivalanalysiswasperformedbytheKaplan-MeiermethodandCoxregressionmodel.Results:LowBMIbeforetreatmentwassignificantlyassociatedwithpooroverallsurvivalinpatientswithLSCC(P<0.001).BMIwasanindependentprognosticfactorforpatientswithLSCC.Conclusion:LeannessbeforetreatmentwasassociatedwithpoorprognosisinpatientswithLSCC.GoodnutritionalstatusisfavorabletoimprovesurvivalinpatientswithLSCC.

简介：无

简介：MicroRNAs是否定地在post-transcriptional水平调制基因表示的短规章的RNA，并且深深地涉及癌症的几种类型的致病。调查特定的miRNAs和他们的目标基因是否参予喉的癌的分子的致病，oligonucleotidemicroarrays被用来与正常纸巾相比在喉的癌纸巾估计microRNAs和mRNAs的微分表示侧面。oncogenicmiRNA，microRNA-21(miR-21)，被发现是在喉的癌纸巾的upregulated。由特定的antisenseoligonucleotides的miR-21击倒而miR-21的overexpression提高了细胞的生长活动，由殖民地形成试金检测了，禁止了HEp-2细胞的增长潜力。miR-21抑制引起的房间数字减小由于G1-S阶段转变的控制的损失，而不是apoptosis的显著增加。随后，miR-21的新目标基因，BTG2，被发现是在喉的癌纸巾的downregulated。BTG2被知道充当一个平底锅房间周期管理者和肿瘤suppressor。这些调查结果显示miR-21的那异常表情可以由维持BTG2的底层贡献喉的癌的恶意的显型。oncogenicmiR-21和它的目标基因的鉴定，BTG2，为癌症诊断和治疗在喉的癌是潜在地珍贵的。

简介：Objective:Laryngealreconstructionisneededtopreservelaryngealfunctioninpatientswhohaveundergoneextensiveverticalorfrontalpartiallaryngectomy.However,theprocedureremainsadifficultchallenge.Severalreconstructiontechniqueshavebeendescribed,butthesetechniquesposerisksofcomplicationssuchaslaryngealstenosis.Thisstudyaimedtoevaluatethepostoperativecourseandfunctionaloutcomesofanewtechniquethatcombinedamuscle-pediclehyoidboneandathyrohyoidflapduringlaryngealreconstructionaftertumorresection.Methods:Fourpatientsunderwentextensiveverticalpartialorfrontalpartiallaryngectomyforcancer.Aftertumorresection,laryngealreconstructionwasperformedusingtheproposedtechnique.Postoperativerecoverytime,complications,andoncologicresultswereevaluated.Results:Thefourpatientsweresuccessfullytreatedwiththeproposedtechnique.Nodyspnea,dysphagia,ordeathoccurredduringthepostoperativecourse.Decannulationwasperformedafteramedianof3days.Theaveragepostoperativehospitalstaywas7days.Short-termpostoperativefunctionalrecoverywasnormal.Nolaryngealstenosisortumorrecurrencewasobservedinanyofthefourpatientsafterafollow-upperiodofmorethan24months.Conclusion:Thecombinationofthemuscle-pediclehyoidboneandthethyrohyoidflapisareliableprocedureforlaryngealreconstructionafterextensiveverticalpartialorfrontalpartiallaryngectomy.

简介：Objective:TolookforthefurtherevidenceforHPVL1HPV16E6,HPV18E6andEBVascarcinogenicfactorsinlaryngealcarcinoma.Method:weexaminedrepresentativenumbersofspecimensfromlaryngealcancerwithhighlysensitivePCRtechniqueforthepresenceofHPVL1andhigh-risktypesHPV16E6,HPV18E6andEBVLMP1.Results:UsingPCRdetection,7.3%sampleswereHPVL1positive,52.03%wereHPV16E6positive,30.89%wereHPV18E6positiveand9.13%wereEBVLMP1positive.ThelowincidenceofHPVL1andhighincidenceofHPV-16E6andHPV18E6genessuggestthatHPVmightbeintegratedintotumorcells.OurresultssupportaroleofHPV-16andHPV-18infectioninthepathogenesisoflaryngealcarcinomainChina.Conclusion:IntegrationofE6intohostgenomeandstableexpressionofthesegenesmaybeassociatedwiththecarcinogenesisoflaryngealcarcinoma.HPV-16andHPV-18maysynergisticallyfunctiononthepathogenesisoflaryngealcarcinoma.Ourresultssuggestanassociationoflaryngealcarcinogenesisandinfectionwiththehigh-riskHPVtypes16,HPV18andEBV.