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  • 简介:High-densitymarkersarenecessaryformap-basedcloningofricegenes,butthecurrentlyavailablemarkersarenotsatisfactoryenough.InDel(insertion-deletionlengthpolymorphism)andSNP(singlenucleotidepolymorphism)arethenewgenerationofmolecularmarkersandcanbasicallymeettheneedoffinemapping.InDelandSNPmarkerscanbedevelopedthroughbioinformatics.Thesemarkersarevaluablemarkerswiththecharactersoflowcost,highspecificityandstability.ThisarticleintroducedthemethodsfordesigningInDelandSNPmarkers,takingthemappingofaricerolledleafgeneasanexample.Inaddition,somekeyfactorsinimprovingthedesignefficiencywerealsodiscussed.

  • 标签: rice molecular MARKER insertion-deletion length POLYMORPHISM