简介:High-densitymarkersarenecessaryformap-basedcloningofricegenes,butthecurrentlyavailablemarkersarenotsatisfactoryenough.InDel(insertion-deletionlengthpolymorphism)andSNP(singlenucleotidepolymorphism)arethenewgenerationofmolecularmarkersandcanbasicallymeettheneedoffinemapping.InDelandSNPmarkerscanbedevelopedthroughbioinformatics.Thesemarkersarevaluablemarkerswiththecharactersoflowcost,highspecificityandstability.ThisarticleintroducedthemethodsfordesigningInDelandSNPmarkers,takingthemappingofaricerolledleafgeneasanexample.Inaddition,somekeyfactorsinimprovingthedesignefficiencywerealsodiscussed.