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简介：Rabbitsareverycute.Theyhavetwolongearsandtworedeyes.Theyhavefourlegsandasmalltail,too.Theycanrunandjump.Theylikecarrots.Ilikerabbitsverymuch.指导老师:周兆群

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简介：瞄准：决定是否输精管切除术离开epididymal尾巴(经由腹股沟的运河)能在精子发生上在兔子减少早手术后的效果。方法：29只正常雄的日本白兔子(46个月)受到单方结束结束(常规)或无限制(juxta-epididymal管的切割结束推迟ens没绑扎)经由腹股沟的运河的输精管切除术。在操作，睾丸，epididymides和管以后的十天和3个月一个defer统一体被移开并且methacrylate准备的嵌入树脂的节。睾丸，epididymis和管的组织学推迟ens在轻显微镜下面被检验，并且生精的小管的体积和直径是用立体声学习的份量上逻辑方法。结果：任何一个输精管切除术的方法都没与相反地侧面的假冒操作方面，而是juxta-epididymal管比较在vasectomized方面上导致明显的损坏到精子发生推迟vasectomized方面上的ens高度被扩张并且包含了众多的精子在操作以后的3个月。结论：离开尾epididymis的输精管切除术没在兔子在精子发生上有重要早手术后的效果。

简介：ObjectivesItisnotfullyclarifiedhowdiabetesmellitusinducedcardiacdysfunctionandmyocardialultrastructuralchangesintheearlystate.Inthepresentstudy,weprovidedanintegratedapproachtoinvestigateearlychangesinmyocardialfunctionofdiabeticrabbitsandassessedthestructuralalteration.MethodsandResultsDiabeteswasinducedbyalloxaninjection.After30days,echocardio-graphyandleftventricularcannulationwereperformedindia-betic(D,n=8)andcontrolrabbits(C,n=10).Aftercatheterization,animalswerekilledforhistologicalstudies.Hema-toxylin-eosinandMasson’sTrichromestainingoftheheartwereanalyzed.Theultrastructureofleftventriclewasalsoexaminedwithelectronmicroscopy.EchocardiographyrevealedthatearlydiabeticcardiomyopathyhadimpairedLVdiastolicfunctionexpressedbydiminishedE-waves,increasedAwaves,E/AratioreversionandincreasedE-wavedecelerationtime(EDT).Concurrently,LVend-diastolicpressure(LVEDP)anddiastolictimeconstant(T)wereincreased,minimumdP/dt(LV-dp/dt)wasreduced,obtainedthroughcardiaccatheterization.TherewerenosignificantdifferencesinLVejectionfraction(EF),LVpeaksystolicpressure(LVSP),ormaximumdP/dt(LV+dp/dt).Qualitativelightmicroscopyrevealednohistologicchangesinmyocardiumfromdiabeticrabbits.Themostevidentultrastructuralchangewasspottedmyofibrillardamage,whileinterstitialfibrosiswasslight.ConclusionsTheseresultssuggestthatearlydiabeticcardiomyopathyinanimalmodelischaracterizedbyleftventriculardiastolicdysfunction,bothimpairedactiverelaxationandincreasedpassivechamberstiffness.Whereas,leftventricularsystolicfunctioncanremainnormal.Itmightpartlycontributetomyofibrillardamage,butnotmyocardialfibrosis.

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简介：Objective:Toobservetheeffectofoctyl-a-cyanoacrylateuponbonehealinganditsdegradationinvitroaftermiddletibialtransversefractureinrabbits,andtoestablishtreatmentofhigherefficacywiththeapplicationofoctyl-a-cyanoacrylate.Methods:MiddletibialtransversefracturemodelofNewZealandrabbitswasestablished.Intheexperimentalgroup,internalfixationwith2mmKirschnerwireswasperformedandthebrokenendswerefixedwithoctyl-a-cyanoacrylate.Inthecontrolgroup,onlyinternalfixationwith2mmKirschnerwireswasconducted.Animalswerekilledatpresettimeintervalsof2,4,6,8,10and12weekspostoperativelyandsampleswereharvested.Results:Twoweeksafteroperation,clearfracturelineswereobservedinboththeexperimentalandthecontrolgroups.Fibroussofttissueconnectionwasnotedbetweenthebrokenendsandtherewassofttissueadhesionaroundthefracturesite.Therewasnocallusformationandthebrokenendsweresurroundedbyadhesivesofttissues.Obviousexternalcallusformationwasconfirmedat8weeksafteroperationinbothgroupswithpartialdisappearanceoffracturelines.Tenandtwelveweeksaftertheoperation,fracturelinesdisappearedcompletelyandtherewasobviousexternalcallusformationandboneunion.Inthefourthweek,fibrouscellsandchondrocyteswerefoundtogrowintothecolloidandsurrounditatthe6thweek.Theadhesivematerialwasdegradedandgraduallyabsorbedatthe8thweek.Chondrificationwasobserved.Conclusions:Twoweeksafterfixationfortibialfractureinrabbits,octyl-a-cyanoacrylatebeginsinvivodegradation.Chondrocytesandfibrocytesgraduallygrowintothedegradationareaandsurroundtheadhesivematerial,whichbrokeintopiecesat8weeks.Completedegradationanddisappearanceoftheadhesivematerialispresentbetween10and12weeks.Nobarriereffecthamperingfracturehealingisnoted.

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简介：Toinvestigatetheelectricalremodelingandtheeffectsofamiodaroneandlosartanonelectricalremodelinginrapidatrialpacingonrabbitmodel.Methods40normalrabbitswererandomlydividedinto4groups:thesalinegroup(controlgroup),amiodaronegroup,losartangroup,ami+losgroup.Allrabbitswereraiseddrugsinaweek.Theatrialeffectiverefractoryperiod(AERP)wasmeasured.Then,takearapidatrialpacing(600bpm)andtheAERPwasmeasuredafter0.5,1,2,4,6and8hourspacingand30minutesaftertheterminationofrapidpacing.Results①Incontrolgroup,after8hoursrapidpacing,AERP200andAERP150weresignificantlyshortened16.11%±3.1%(P<0.01)and9.99%±4.2%(P<0.01).AndthedegreeofAERPshorteninginducedbyrapidpacingwasgreateratbasiccyclelengthsof200ms(BCL200)thanthatatBCL150.TheAERPofamiodarone,losartangroupandami+losgroupwerenotshortenedduringrapidpacing.②Inthecontrolgroup,aftertheterminationofrapidpacing,theAERPgraduallyincreased.TheAERPatalloftheBCLSexaminedrecoveredtoalmostthe95.78%and96.76%ofbaselinevalueswithinthefirst10minutesandrecoveredtoalmostthe99.07%and99.39%ofbaselinevalueswithinthefirst30minutes.ConclusionsShort-termatrialrapidpacingcaninducetheatrialelectricalremodeling.Amiodaroneandlosartancanpreventtheelectricalremodeling.

简介：ObjectiveTo评估未耕作的脂肪质导出stromal的潜在的效果腱healing.MethodsTwenty上的脉管的部分称2.5-3.0kg的五只成年雄的新西兰白兔子被使用。当脂肪质的组织和剩余的施主被划分成控制和治疗组，五只兔子被使用。损害模型被单方的腱切断术通过中间完成三分之一深数字的屈肌腱。立即在缝术以后修理，也新鲜的stromal从脂肪质的织物或安慰剂的酶的消化的脉管的部分intratendinously分别地在治疗和控制组在腱树桩被注射。有演员组的固定在外科以后被继续二个星期。动物在外科以后在八个星期被牺牲，腱经历了组织学，immunohistochemical，和机械评估。量、质的数据的统计分析用变化和Mann-Whitney的单程的分析被估计[/-test，表明的优异纤丝状的线性和连续性，和在处理的减少的由脉管形成组织的respectively.ResultsHistological评估显示了改进组织并且neotendons改变。Immunohistochemistry在骨胶原表明了重要增加我在处理的表示组。最终的负担和精力吸收能力显著地两个都与学习显示出的在场的controls.ConclusionThe相比在对待房间的修理被增加未耕作的脂肪质导出stromal的那intratendinous注射脉管的部分结果在改进了腱修理和它的结构、机械的性质能是为对待腱损害的一种有效形式。

简介：Objective:Todeterminetheefficacyofpolylacticacidglueinpreventingepiduralscaradhesionafterlaminectomyinrabbits.Methods:Twenty-fourJapanesewhiterabbitsunderwentlaminectomy(includingtheattachedligaments)atL2andL5.AfterlaminectomyatL5,polylacticacidgluewassprayedontheduraandnerverootsandthissegmentwastakenastheexperimentalgroup.AfterlaminectomyatL2,nothingwasusedandthissegmentwasenrolledastheselfcontrolgroup.Fourrabbitswerekilledeverytwoweekspostoperativelytilltheendoftheexperimentat12weeks.Thentheoperatedspinewasobservedgrossly,histologicallyandultrastructurallytocheckthedegreeofscarformation,thestatusofepiduralscaradhesion,theabsorptionoftheglue,andtheintracellularstructureoffibroblasts.Results:Thegluecoagulatedimmediatelyaftersprayingandshowedexcellenthemostaticeffect.Thegluemembranewaseasytobetakenawayfromtheduramaterofthesamplesfor2weeksandtherewerenocellsintheepiduralspaceintheexperimentalgroup.Buttheduramaterwascoveredbyhematomainthecontrolgroup,whichformedmildadhesion,withfibroblastsproliferatingactively.Inthe4thweek,someglueshiversremainedintheepiduralspacewithfibroblastsincreasingalittle,andtheduramaterwassmoothintheexperimentalgroup.However,inthecontrolgroup,theformedscarwasfragileandconglutinatedwiththeduramaterdiffuselyandfibroblastsweremuchmorethanthoseintheexperimentalgroup.Inthe6th-12thweeks,therewasapotentialinterspacebetweenthescarandtheduramater,andthepolylacticacidgluewasabsorbedcompletelyintheexperimentalgroup.Muchtoughscarwasfoundinthecontrolgroup,whichwasverydifficulttodissectfromtheduramaterandthesurroundingtissues.Fromtheultrastructuralobservationofthefibroblasts,thenucleusbecamemuchbiggerandtheroughendoplasmicreticulumwasmuchmoreplentifulinthecontrolgroupthanthatinthee

简介：瞄准：为了调查超级顺磁的氧化铁(SPIO)的生物进程是否把人的间充质的干细胞(hMSCs)标记，可以被使用在活体内non-invasively监视有在老鼠和兔子的阴茎海绵体的常规1.5-T系统考试的磁性的回声(先生)成像。方法：分别地与普鲁士的蓝色和Tripan蓝色检验的标记SPIO的hMSCswere的标记的效率和生存能力。在标记SPIO的hMSCs被移植到老鼠和兔子的阴茎海绵体以后，连续T2加权的先生图象被拿，组织学的考试在一个4星期的时期上被执行。结果：与SPIOcompared装载到未标记的房间的hMSCs有一个类似的生存能力。为标记SPIO的hMSCs超过1x10~5concentration在试管内，先生图象在信号紧张显示出减少。在在老鼠和兔子阴茎海绵体的标记SPIO的hMSCs的区域的先生信号紧张局部地减少了并且被限制。在进阴茎海绵体的标记SPIO的hMSCs的注射以后，先生成像证明hMSCs能在注射以后被看见至少12个星期。铁的存在被证实，普鲁士的蓝色在组织学的节染色。结论：在老鼠和兔子的阴茎海绵体的标记SPIO的hMSCs能被分子的先生成像non-invasively评估。我们的调查结果建议先生成像有能力在可勃起的机能障碍的背景在动物测试hMSCs的长期的治疗学的潜力。

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简介：Thepresentworkisaimedtostudythepharmacokineticparametersofoptimizedrepaglinidefloatingdrugdeliverysystem(FDDS)by24factorialdesigns,followedbycomparisonwithacommerciallyavailableformulation.Themaineffectsandinteractionsofformulationvariableswerestudiedbyusingnormalandparetocharts.Theoptimizedformulationshowsafickiandiffusiondrugreleasemechanism.Pharmacokineticparametersofthedesigneddrugdeliverysystemwereevaluatedinrabbitmodels.Meanwhileasimple,specifichighperformanceliquidchromatographicmethodwasdevelopedandvalidatedasperbiopharmaceuticalspecifications,thelinearitywasobservedattherangeof110-550ng/mL(r2=0.999).Byusingmethanol-phosphatebuffer(pH2.5)(70:30,v/v)asmobilephaseattheflowrateof1.0mL/minthevalidationshowsabetterretentiontimeof5.2minforrepaglinide.AndthesamevalidationmethodwasusedforpharmacokineticprofileanalysisofrepaglinidemarketedproductsandFDDS.Thecomparativepharmacokineticresultssuchastmax,half-life,areaunderthecurve,meanresidencetimeswereincreasedsignificantlyfortherepaglinideinFDDSthanthemarketedproductofrepaglinideexceptCmaxandeliminationrateconstant.

简介：BACKGROUND:ThepharmacologicalactionoftraditionalChinesemedicinecompoundisthecomprehensiveeffectofthevariousingredients,andtheinteractionsofvariousingredientsarecloselycorrelatedwiththefinaleffect.InordertorevealthecompatibilitymechanismofBHD'sprescriptionintreatingandpreventingischemiccerebrovasculardisease,weneededexploretheeffectandrelationofingredientsintheprescription.OBJECTIVE:ToobservetheeffectofBuyangHuanwudecoction(BHD)andAstragalusmongholicusontheactivityofplateletactivatingfactorreceptor(PAFR)intheplateletofrabbitsinvitro,andinvestigatethemechanismofAstragalusmongholicus.DESIGN:Adecomposedrecipesstudy.SETTING:GuangzhouUniversityofTraditionalChineseMedicine.MATERIALS:FiveNewZealandrabbits,weighing2-3kg,bothsexes,wereused.BHDwascomposedofShengHuangQi120g,DangGuiWei6g,ChiShao4.5g,ChuanXiong3g,DiLong3g,TaoRen3g,HongHua3g.TheprescriptionforactivatingbloodcirculationconsistedofDangGuiWei6g,ChiShao4.5g,ChuanXiong3g,DiLong3g,TaoRen3gandHongHua3g.Theprescriptionforinvigoratingqiconsistedof120gShengHuangQi.ThepreparedherbalpieceswerepurchasedfromthetraditionalChinesemedicineDispensaryofFoshanSecondPeople'sHospital,andappraisedbyProfessorXufromScienceofChineseMateriaMedicaCollege,GuangzhouUniversityofTraditionalChineseMedicine.3H-PAFwassuppliedbyAmershamCo.,Ltd.(specificactivity:6.475TBq/mmol;batchnumber:200402);PAFstandardbyBiomolCo.,Ltd.(batchnumber:P1318V).METHODS:TheexperimentswerecarriedoutintheLaboratoryofNuclearMedicine,GuangzhouUniversityofTraditionalChineseMedicinefromSeptembertoDecember2004.①InjectionsofBHD,prescriptionsforactivatingbloodcirculationandinvigoratingqiwerepreparedbythedecoctionandalcoholsedimentationtechnique.Rabbitcommoncarotidarteryblood(40mL)wasdrawnviaintubationtoprepareplateletsuspensionof(0.8-1.0)×1010L-1.②Determinationo

简介：客观：在兔子在针的绳索ischemia/reperfusion(I/R)以后在lipidperoxidation和apoptosis上学习白果树biloba摘录(GBE)的效果。方法：SpinalcordI/R损害模型根据对Erten等的描述被建立。27只NewZealand白兔子的一个总数随机被划分成三个组：一个假冒的组(9只兔子对待withs火腿操作但是没有大动脉的吸藏)，一个模型组(与大动脉的吸藏对待的9只兔子并且匹配卷盐)，并且一个GBE组(与大动脉的吸藏andGinaton(100mg/kg)对待的9只兔子在大动脉的夹钳前并且在灌注的发作注射了30分钟)。Theneurological结果分别地在灌注以后在24和48个小时被评估。针的绳索malondialdehyde(MDA)水平，超级氧化物dismutase(草皮)然后被检测。神经cellapoptosis被终端deoxynucleotidyltransferase(TdT)决定标记的-mediateddUTP-fluorescence刻痕结束(TUNEL)方法和bcl-2和bax的表示是examinedhistologically在有免疫组织化学的针的绳索。结果：I/Rproduced在神经病学的得分的重要减少。GBE组的马达分数比在在灌注以后的24和48个小时的模型组的那些显著地高(P<0.05)。与模型组相比，GBE改善了草皮的下面规定并且生产了MDA水平的重要减小(P<0.01)。为在模型组的TUNEL的积极房间是多于GBE组的那些的大部分(P<0.01)。bcl-2在I/R以后是起来调整的，特别在theGBE组(P<0.01)。bax的起来规定被GBE极大地减少(P<0.01).Conclusions：GBE对针的绳索I/R损害，和机制有保护的效果可以是它能清除氧释放激进分子并且禁止神经房间的apoptosis。

简介：Toinvestigatetheeffectsofsimvastatinonmembraneioniccurrentsinleftventricularmyocytesofrabbitheartsufferingfromacutemyocardialinfarction(AMI),soastoexploretheionicmechanismofstatintreatmentforantiarrhythmia.MethodsForty-fiveNewZealandrabbitswererandomlydividedintothreegroups:AMIgroup,simvastatininterventiongroup(Statingroup)andsham-operatedcontrolgroup(CON).Rabbitswereinfarctedbyligationoftheleftanteriordescendingcoronaryarteryafteradministrationoforalsimvastatin5mg·kg-1·d-1(Statingroup)orplacebo(AMIgroup)for3days.Singleventricularmyocyteswereisolatedenzymaticallyfromtheepicardialzoneoftheinfractedregion72hlater.Wholecellpatchclamptechniquewasusedtorecordmembraneioniccurrents,includingsodiumcurrent(INa),L-typecalciumcurrent(ICa-L)andtransientoutwardpotassiumcurrent(Ito).Results①Therewasnotsignificantdifferenceinserumcholesterolconcentrationamongthreegroups.②ThepeakINacurrentdensity(at-30mV)wassignificantlydecreasedinAMIgroup(-25.26±5.28,n=13),comparingwithCON(-42.78±5.48,n=16),P<0.05,whileitwassignificantlyincreasedinStatingroup(-39.83±5.65pA/pF,n=12)comparingwithAMIgroup,P<0.01;ThepeakICa-Lcurrentdensity(at0mV)wassignificantlydecreasedinAMIgroup(-3.43±0.92pA/pF,n=13)comparingwithCON(-4.56±1.01pA/pF,n=15),P<0.05,whileitwassignificantlyincreasedinStatingroup(-4.18±0.96pA/pF,n=12)comparingwithAMIgroup,P<0.05;TheItocurrentdensity(at+60mV)wassignificantlydecreasedinAMIgroup(11.41±1.94pA/pF,n=13)comparingwithCON(17.41±3.13pA/pF,n=15),P<0.01,whileitwassignificantlyincreasedinStatingroup(16.11±2.43pA/pF,n=14)comparingwithAMIgroup,P<0.01.ConclusionsAMIinducessignificantdown-regulationofINa,ICa-LandIto.Pretreatmentwithsimvastatincouldattenuatethischangewithoutloweringtheserumcholesterollevel,suggestingthatsimvastatincouldrev

简介：Objective:Toevaluatetheprotectiveeffectsof8%emulsifiedisofluraneaftermyocardialischemia-reperfusioninjuryanditsmechanisminrabbits.Methods:Twenty-fourmaleadultNewZealandwhiterabbitswereanesthetizedwithintravenousinjectionof30mg/kgpentobarbitalfollowedby5mg·kg-1·h-1infusion.Allrabbitsweresubjectedto30minutesofleftanteriorde-scendingcoronaryartery(LAD)occlusionand3hoursofsubsequentreperfusion.BeforeLADocclusion,therabbitswererandomlyallocatedintothreegroupsforprecondi-tioningtreatment(eightforeachgroup).Thecontrolgroup(Cgroup)receivedintravenously0.9%NaClfor30minutes.Theemulsifiedisofluranegroup(EIgroup)received8%emulsifiedisofluraneintravenouslytill0.64%end-tidalcon-centrationfor30minutesthatwasfollowedbya15-minutewashoutperiod.TheIntralipidgroup(INgroup)received30%Intralipidfor30minutes.Theinfarctedarea,plasmamalondialdehyde(MDA)content,superoxidedismutaseactivity(SOD)andnitriteconcentrationafter3-hourmyo-cardialperfusionwererecordedsimultaneously.Results:Forthemyocardialischemia-reperfusionin-juryanimals,theinfarctedsizeintheEIgroupwassignifi-cantlyreduced(91.9%±8%)ascomparedwithcontrolgroup(39%±6%,t=5.19,P<0.01).TheplasmaSODactivityandnitriteconcentrationinEIgroupweresignificantlyhigherthanthoseincontrolgroup(t=2.82,t=8.46,P<0.05),butMDAcontentwaslowerinEIgroupthanthatincontrolgroup(t=2.56,P<0.05).Conclusions:Theresultsindicatethatemulsifiedisofluranehasacardioprotectioneffectagainstischemia-reperfusioninjury.ThisbeneficialeffectofemulsifiedisofluraneisprobablythroughNOreleaseandconsequentlybyincreaseinautioxidationofmyocardium.

简介：Objective:Toobservethetherapeuticeffectofelectroacupuncture(EA)onplasmaangiotensin(Ang*.Ⅱ),aldosterone(ALD)andatrialnatriureticpolypeptide(ANP)contentsinexperimentalcerebralinfarctionrabbitsforanalyzingtheunderlyingmechanismofacupunctureinamelioratingbloodsupplyofthebraintissue.Methods:Atotalof80rabbitswererandomizedintocontrol(n=8),pseudo-operation(n=24),model(n=24)andEA(n=24)groups.Cerebralinfarctionmodelwasestablishedbyinfusionofself-thrombusintothecarotidartery.EA(1mA,2Hz)wasappliedto'Baihui'(百会GV20)and'Shuigou'(水沟GV26)for30min,onceevery12hours.PlasmaAng-II,ALDandANPcontentsweredetectedwithradioimmunoassaymethod.Inthelater3groups,bloodsamplesweretakenat6h,24hand48haftercerebralischemia.Results:Comparedwithcontrolandpseudo-operationgroups,Ang-IIandALDcontentsofmodelgroupat6h,24hand48haftercerebralischemiaincreasedsignificantlywhileplasmaANPofthe3time-coursesofmodelgroupdecreasedconsiderably(P<0.01).Incomparisonwithmodelgroup,resultsshowedthatAng-IIandALDcontentsofEAgroupdecreasedsignificantlywhereasANPlevelofEAgroupincreasedstrikingly(P<0.01).Conclusion:ElectroacupuncturehastheeffectsofraisingplasmaANPlevelandloweringplasmaAng-IIandALDincerebralinfarctionrabbits.

简介：Objective:Toexploretheeffectofdexamethasonebylocaltreatmentofcerebraledemaandbraindamageafterbraininjury.Methods:Twenty-tworabbitswereclassifiedinto2groups,GroupA(thecontrolgroup,n=11)andGroupB(thetreatedgroup,n=11).Anrabbitbraincontusionmodelwasmadebybonewindowplastybyextraduralhitting.Groupbwastreatedbylocalinfiltratingandsprayingofdexamethasoneatequidistancetolesions.GroupAwasgivennormalsalineinthesamewayasGroupB.Thechangesofmoistureinbraintissuesandserummyelinbasicprotein(MBP)wereobserved.Results:ThepercentageofwatercontentindamagedhemisphereinGroupAandGroupbwas81.7%±0.56%and79.45%±0.52%respectively.Therewasasignificantdifferencebetweenthe2groups(P<0.05).ThenormallevelofMBPwas1.66μg/L±0.71μg/L,whilethevalueofMBPinGroupAandGroupbwere5.98μg/L±2.08μg/Land3.15μg/L±1.09μg/Lseparately.ThelevelofMBPinGroupAandGroupBwerehigherthannormallevelandtherewasalsoasignificantdifferencebetweenGroupAandGroupB(P<0.05).Conclusions:TheresultsofourstudyshowedthatthebrainmoistureandMBPinserumwereincreasedafterbraininjurywhilereducedaftertreatmentwithdexamethasone.ItisdemonstratedthatlocaltreatmentofbraininjurywithdexamethasonehasanobvioustherapeuticeffectoncerebraledemaandserumMBP.