简介：将节杆菌和芽孢杆菌分别暴露于不同浓度的铅溶液中,并进行不同暴露时间的急性毒性试验。结果表明,500mg/kg的Pb（II）处理会使节杆菌中的细胞膜脂质过氧化产物——硫代巴比妥酸活性物质（TBARS）浓度显著降低（p〈0.05）,而Pb（II）质量比变化对其他指标无显著影响（p〈0.05）;铅处理会使芽孢杆菌中可溶性蛋白质量浓度、GSH质量浓度和超氧化物歧化酶（SOD）活性显著升高,而过氧化氢酶（CAT）活性显著降低（p〈0.05）。节杆菌的可溶性糖质量浓度、GSH质量浓度、SOD活性和TBARS浓度随暴露时间延长而显著增加;芽孢杆菌的可溶性糖质量浓度、GSH质量浓度和SOD活性随暴露时间延长而显著增加,TBARS浓度随暴露时间延长呈先增后减的变化趋势,CAT活性也表现出先增后降的变化趋势。铅处理对节杆菌和芽孢杆菌具有一定的胁迫作用,两种菌通过启动不同的抗性系统来抵抗外界胁迫。

简介：ＡＮＥＷＣＥＲＡＭＩＤＥＦＲＯＭＴＨＥＭＡＲＩＮＥＳＰＯＮＧＥＢＩＥＭＮＡＳＰ．￥ＺｈｉＺＥＲＧ；ＬｏｎｇＭｅｉＺＥＮＧ；ＪｉｎｇＹｕＳＵ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＺｈｏｎｇｓｈａｎＵｎｉｖｅｒｓｉｔｙ，Ｇｕａｎｇｚｈｏｕ，６１０...

简介：2004年4月16日笔者从江苏省泰兴市国庆东路一户外取回一木材，从木材内分拣得散白蚁Reticulitermessp.71头，放在一玻璃瓶中进行观察(该瓶内径为2cm，高为5cm)，其中仅有六头为兵蚁(其中有一只腹部被碰了一下，不过还活着)，其余均为工蚁。

简介：现在的学习瞄准了估计与海洋的海绵联系的细菌的antifouling活动。八细菌的紧张的一个总数从海绵Sigmadociasp的表面被孤立，他们，SS02，SS05和SS06对形成biofilm细菌显示出禁止的活动。这3紧张的摘录更加影响了生产形成biofilm的能力和粘附的细胞外的聚合物质细菌的紧张。除了圆盘散开试金，microalgal解决试金被执行，摘录与聚氨酯木头光泽混合了并且到不锈钢赠券上涂。紧张SS05的摘录显示出强壮的microalgal解决禁止的活动。紧张SS05基于它的16SrRNA基因作为杆菌仙影拳被识别。与海洋的无脊椎动物联系的细菌的紧张的代谢物答应被发展进环境友好的antifouling代理人。

简介：

简介：目的：研究一株分离自条斑紫菜丝状体上的真菌代谢产物的活性成分。方法：利用电镜观察真菌形态特征,利用MEA培养基进行大规模发酵,经硅胶层析、葡聚糖凝胶层析等各种层析手段分离化合物,通过波谱技术鉴定化合物结构,并利用MTT法测定化合物抗肿瘤活性。结果：该菌属于曲霉属,分离得到了两个化合物1和2,分别为cyclo（L-Trp-L-Phe）（1）和WIN64821（2）。MTT法测试结果表明,cyclo（L-Trp-L-Phe）和WIN64821对37株人体肿瘤细胞株具有中等细胞毒活性,其平均IC50分别为3.3和5.8μg·mL^-1。结论：第一次报道这两种化合物的抗肿瘤活性。

简介：目的：对采自中国南海的海绵Axinyssasp．进行化学成分研究，从中寻找具有生物活性的次生代谢产物。方法：用硅胶和凝胶LH-20柱层析对海绵Axinyssasp．的乙醚提取物进行分离纯化，并结合现代波谱技术对得到的化合物进行结构鉴定。结果：分离得到了4个化合物，其结构分别鉴定为theonellinisothiocyanate（1），theonellinformamide（2），7-isothiocyanato-7，8-dihydro-α-bisabolene（3）和axinysterol（4）。结论：化合物1-3均为首次从海绵Axinyssasp．中分离得到，同时也是首次从中国海域的海洋生物中分离得到。

简介：AnewmethodforintrinsicterminatorpredictionbasedonRnall,anRNAlocalsecondarystructurepredictionalgorithmdevelopedrecently,andtwoU-tailscoreschemasaredeveloped.Byoptimizingthreeparameters(thermodynamicenergyofRNAhairpinstructure,U-tailTweight,andU-tailhybridizationenergy),themethodcanrecognize92.25%ofknownterminatorswhilerejecting98.48%ofpredictedRNAlocalsecondarystructuresincodingregions(negativecontrol)asfalseintrinsicterminatorsinE.coll.ThismethodwasappliedtoscanthegenomeofSynechocgccussp.WH8102,andwepredicted266intrinsicterminators,whichincluded232protein-codinggenes,12tRNAgenes,and3rRNAgenes.About17%oftheseterminatorsarelocatedattheendofoperons.Itisalsoidentified8pairsofbiodirectionalterminators.ThemethodforintrinsicterminatorpredictionhasbeenincorporatedintoRnM1,whichisavailableathttp://digbio.missouri.edu/-wanx/Rnall/.

简介：

简介：Inthisresearch,thedegradationofdibenzothiophene(DBT)wasinvestigatedbyusingPseudomonassp.LKY-5isolatedfromoilcontaminatedsoil.Theresponsesurfacemethodology(RSM)basedontheBox-Behnkendesign(BBD)wasappliedforevaluatingtheinteractiveeffectsoffourindependentvariablesincludingsubstrateconcentration,temperature,pHandagitationrateontheDBTremovalresponse.Atotalof29experimentsforfourfactorsatthreelevelswereconductedinpresentstudy.Asecond-orderregressionmodelwasthendeveloped,andtheanalysisofvariance(ANOVA)illustratedthattheproposedquadraticmodelcouldbeutilizedtonavigatethedesignspace.Thevalueofdeterminationcoefficient(R2=0.9534)indicatedasatisfactoryagreementbetweenthequadraticmodelandtheexperimentaldata.ItwasfoundthatDBTremovalwasmoresignificantlyaffected(P<0.0001)bysubstrateconcentrationcomparedwithotherthreeparameters.An100%degradationofDBTcouldbeobtainedbyPseudomonassp.LKY-5atasubstrateconcentrationof100mg/L.

简介：Inthispaper,thetotalsugarconcentration,proteinconcentration,lipidyieldandmorphologycharacteristicsofthealgaeresidueweredeterminedtoexplainthemechanismoflipidsextractionfromwetmicroalgaeScenedesmussp.byionicliquidassistedsubcriticalwater.Theresultsshowedsimilarvariationforthesugar,proteinandlipid.However,thetotalsugarwasmoresimilartolipidsyield,sotheresultsshowedthatthereactionbetweenionicliquidandcelluloseandhemicelluloseincellwallwasthemostimportantstepwhichdeterminedthelipidsextrationdirectly.Andthetotalsugarvariationmayberepresentingthelipidsyield.Forlaterlipidsextraction,wecandeterminethetotalsugarconcentrationtopredicttheextractionendproduct.

简介：Laurenciananhaiensesp.nov.(Rhodomelaceae,Rhodophyta)isdescribedfromHainanandGuangdongProvinces,China.Thenewspeciesclearlydisplaysoneofthedefiningfeaturesofthegenus,viz.fourperiaxialcellspervegetativeaxialsegment.Itdiffersfromothercloselyrelatedspeciesinhavingacombinationoffeaturessuchastereteaxesfromabasalsystemcomposedofaprimarydiscoidholdfastandasecondaryattachmenttogiverisetomanyshortrhizoids,branchingoppositelyandalternately,irregularlytristichousorsubverticillatelypolystichous,havingmorecurvebrancheswithverysparse,adventitiousultimatebranchlets,non-projectingsuperficialcorticalcellsattheapicesofultimatebranchlets,presenceoflongitudinallyorientedsecondarypit-connectionsbetweencontiguoussuperficialcorticalcells,absenceoflenticularthickeningsinthewallsofmedullarycells,parallelarrangementoftetrasporangiaalongtheaxisofstichidia,andpresenceofintercellularspacesbetweenmedullarycells.

简介：Theobjectiveofthisstudywastoinvestigatetheef-fectsofearthwormdensityontheavailabilityofnutrientsandheavymetalsinmetalcontaminatedsoils.Pb/Znminetailingsweremixedthroughlywitharedyellowpodzolicsoilattheratio(w/w)of75:25.Earthworms(Pheretimasp.)wereintroducedtothemixtureatfourdifferentdensities,zero,three,sixandnineindividualsperpotplantedwithryegrass(Loliunmultiflorum).Theresultsindicatedthatearthwormactivitysignificantlyen-hancedryegrassshootbiomass.However,asdenserearthwormpopulationwasintroduced,shootbiomasstendedtodecrease.EarthwormactivitysignificantlyincreasedsoilpHandavailabilityofN,PandKinthetailingsandsoilmixture.TherewasageneraltendencythatuptakeofZnbyryegrassincreasedafterearthworminoculation,althoughtheincreaseinextractableZnintailingsandsoilmixturewasnotsignificant.Onthecontrary,thereseemedtobealoweruptakeofPbbyryegrassunderearthworminoclation,despitethefactthathigherextractablePbconcentrationswereobserved.Thepresentprojectindicatedthattheimprovedgrowthofryegrasswasduetoimprovednutrientavailabilityandothersoilconditions,byinoculationofearthwormsatanappropriaterate.Furtherstudiesareneededtoillustratetherelationshipbe-tweenmetalavailabilityandearthwormactivityinthefield.

简介：ApotassiumsolubilizingbacterialstraindesignatedEGT,whichistolerantofhightemperature,wasisolatedfromanearthworm’sguttoobtainabacteriumthatcanweatherpotassium-bearingrockeffectivelythroughsolid-statefermentation.Molecularphylogenyand16SrRNAgenesequenceanalysisdemonstratedthebacterialstrainwasamemberoftheStreptomycesgenus.Toassessitspotentialtoreleasepotassiumfromsilicateminerals,thisstrainwasusedtodegradepotassium-bearingrockpowderbysolid-statefermentation.Afterfermentation,theamountofwater-solubleAl,FeandKofthesubstratewithactiveinoculumwashigherthanthoseofthecontrol,whichhadautoclavedinoculum,andthoseofthefreshsubstrate.Theresultindicatedthatthestrainhadtheabilitytoweatherpotassium-bearingrockandcouldbeusedasaninoculumintheproductionofpotassiumbio-fertilizer,duetoitspotassiumreleaseactivityfromrockandtolerancetohightemperature.

简介：Anewhexaketideacidesterifiedbythe17-hydroxylgroupof16,17-dihydroxycyclooctatin,namely17-[16,17-dihydroxycyclooctatinyl]-hexaketideester(1),amemberofthegroupofrarebacterialditerpeneswithafused5-8-5ringsystemwasisolatedfromstrainStreptomycessp.SR107.Thestructurewasdeterminedonthebasisofitsspectraldata(~1HNMR,~(13)CNMR,~1H-~1HCOSY,HSQC,HMBC,NOESY,IRandHR-ESI-MS).Theantibacterialactivitywasalsoevaluatedinthispaper.

简介：生产bioflocculant细菌的79紧张从3activated污泥样品被孤立。在他们之中，紧张MYC被发现有最高、稳定的絮凝的率因为两个都白陶土泥土暂停和油田生产了水。细菌的紧张作为Klebsiellasp被识别。MYC根据它的词法、生物化学的特征和16SrDNA定序。由这细菌的紧张的简历凝聚剂生产的最佳的媒介由藤条糖20gL~组成(-1),KH_2PO_42gL~(-1)K_2HPO_45gL~(-1),(NH_4)_2SO_40.2gL~(-1)urea0.5gL~(-1)和酵母摘录0.5gL~(-1),起始的氢指数5.5。当白陶土泥土的暂停与0.5%Klebsiellasp被对待时。MYC文化肉汤，絮凝的率面对500mgL~到达了超过90.0%(-1)CaCl_2，当为oil-fieldproduced水的絮凝的率在一个氢指数在80.0%附近时，7.0变化-9.0在类似的条件下面与从生产的油田的油和推迟的粒子的分离流水。凝聚剂和紧张的高絮凝率使简历凝聚剂成为的简历的environment-friendlynature由Klebsiellasp生产了。在油田的吸引人的简历凝聚剂生产了的MYC浇处理。

简介：在转基因的过程，外国基因能集成于在二个方向的主机染色体，它可以影响它的表达式。以便学习insertalorientation的效果，gfp记者基因在Synechococcussp的isiAB地点被插入。PCC7942冷淡的方向，和GFP表示层次和转基因的水藻的生长被比较。gfp基因能在每个方向表示，这被显示出，并且没有重要差别在在二recombinant水藻之间的海藻的生长和GFP表示层次上被检测。

简介：新种，白果树huolinhensissp。nov与从更低的白垩纪的保存得很好的表皮，在内部蒙古的Huolinhe煤地的Huolinhe形成，中国，词法上并且解剖地被学习。新种类的主要人物在深深地在叶柄的表皮上与狭窄的脑叶，在上面、更低的表皮的房间上开发的毛状体，在上面的表皮为每个房间在穹状的墙上开发的媒介乳头状的小突起，和没有毛状体被把的叶子被发现。G的这些人物。huolinhensissp。nov。与以前报导的白果树的已知的种类区分开来。新种类的这研究为石块白果树的分类研究是有益的，并且应该对更好理解有用在早白垩纪期间住在东北中国的白果树的paleoenvironment。

简介：

简介：InastudyofmeiofaunapolychaetefromtheHuanghaiSea,apreviouslyundescribedspeciesbelongingtoHesionurahasbeenfoundanddescribed.ThenewspeciesdiffersfromtheotherspeciesofthegenusHesinuramainlybythechaetation.