简介：目的探索肺炎衣原体（chlamydiapneumoniae，Cpn）与鼻息肉之间的关系。方法用直接免疫荧光法检测外周血单核细胞（peripheralbloodmononclearcell，PBMC）中的Cpn特异性抗原（Cpn-Ag），间接微量免疫荧光法检测血浆中Cpn抗体。结果在73例鼻息肉患者和87例正常人血浆中，CpnIgG的阳性检出率分别为65.8％（48／73）和32．2％（28／87），P〈0．001；CpnIgM的阳性检出率分别为30．1％（22／73）和12．6％（11／87），P〈0．01；PBMC中的Cpn-Ag阳性检出率分别为86．3％（63／73）和31．0％（27／87），P〈0．001。结论鼻息肉与肺炎衣原体有一定的相关性。检测PBMC中Cpn—Ag可为更好的治疗鼻息肉提供实验依据。（中国眼耳鼻喉科杂志，2006，6：233-234）

简介：目的探索肺炎衣原体(Chlamydiapneumoniae,Cpn)与急性咽喉感染之间的关系.方法用直接免疫荧光(directimmunofluorescence,DIF)法检测外周血单核细胞中的Cpn特异性抗原,间接微量免疫荧光(microimmunofluorescence,MIF)法检测血浆中Cpn抗体.结果在94例急性咽喉感染患者和87例正常人血浆中,CpnIgG的阳性检出率分别为54.3%(51/94)和32.2%(28/87),P＜0.05;CpnIgM的阳性检出率分别为59.6%(56/94)和12.6%(11/87),P＜0.05;外周血单核细胞中的Cpn特异性抗原阳性检出率分别为62.78%(59/94)和31.0%(27/87),P＜0.05.结论急性咽喉感染与肺炎衣原体有密切关系.检测外周血单核细胞中Cpn特异性抗原可为诊治急性咽喉感染提供实验依据.

简介：<正>在20世纪,耳鼻喉科不仅发展很快,而且对整个医学作出重大贡献:本世纪初气管食管窥镜的成功应用,创建了气管食管学;40年代双目手术显微镜问世导致耳显微外科的诞生;60年代初发现电刺激耳蜗神经引起听觉,发明人工耳蜗植入,至80年代引入微机处理,数字信息技术,产生多道程控人工

简介：目的构建肺炎链球菌自溶素（pneumococcalautolysin，LytA）核酸疫苗，并研究其免疫原性。方法采用聚合酶链反应方法从肺炎链球菌基因组中克隆肺炎链球菌自溶素基因，然后将该基因插入到pVAX1真核表达载体中，构建表达LytA的重组质粒，制备成超螺旋比例大于90％的核酸疫苗。实验组：BALB／c小鼠5只，对其分3次肌内注射核酸疫苗（100μg）；对照组：BALB／c小鼠5只，肌内注射pVAX1空载体（100μg）3次。分别采集小鼠血清，采用间接酶联免疫吸附方法测定血清LytA特异性抗体水平。结果扩增出的LytA基因（957bp）与基因库中LytA的编码序列（957bp）一致。成功构建了LytA核酸疫苗。实验组小鼠的血清LytA抗体水平明显高于对照组，差异有统计学意义（P〈0．01）。结论成功克隆了LytA基因，制备了LytA核酸疫苗，后者可在小鼠体内诱导较强的特异性体液免疫反应。

简介：AIM:Anaerobicbacteriacancauseocularinfections.WetestedtheOxyPlateTMAnaerobicSystem(OXY)toisolatepertinentanaerobicbacteriathatcancauseoculardisease.METHODS:OXY,whichdoesnotrequiredirectanaerobicconditions(i.e.bags,jars),wascomparedtoconventionalisolationofincubatingculturemediainanaerobicbags.Standardcoloniescountswereperformedonanaerobicocularbacterialisolatesunderaerobicandanaerobicconditions(anaerobicbags)usingagarmedia:1)OXY(aerobiconly),2)5%sheepblood(SB),3)Chocolate,and4)Schaedler.Thebacteriatestedwerede-identifiedocularisolatesculturedfromendophthalmitisanddacryocystitisthatinclude10Propionibacteriumacnesand3Actinomycesspecies.Thecolonycountsforeachbacteriaisolate,oneachculturingcondition,wererankedfromlargesttosmallest,andnon-parametricallycomparedtodeterminethebestculturingcondition.RESULTS:Allanaerobicconditionswerepositiveforalloftheanaerobicisolates.SBandSchaedler’sagarunderaerobicconditionsdidnotsupportthegrowthofanaerobicbacteria.SparsegrowthwasnotedonchocolateagarwithPropionibacteriumacnes.Asananaerobicsystem,SBinananaerobicbagisolatedhighercolonycountsthanOXY(P=0.0028)andchocolateagar(P=0.0028).CONCLUSION:AlthoughOXYdidnottesttobemoreefficientthanotheranaerobicsystems,itappearstobeareasonablealternativeforisolatinganaerobicbacteriafromocularsites.Theuseofanagarmediuminaspeciallydesignedplate,withouttherequirementofananaerobicbag,renderedOXYasanadvantageoverotheranaerobicsystems.

简介：AIM:ToIntroduceanewspecializedvisualacuitychartforamblyopicchildrenaged3-5yearsoldanditsclinicalapplications.METHODS:ThenewvisualacuitychartandnotationsweredesignedbasedonWeber-Fechnerlaw.Theoptotypeswereredagainstawhitebackgroundandwerespeciallyshapedfourbasicgeometricsymbols:circle,square,triangle,andcross.Aregulargeometricprogressionoftheoptotypesizesanddistributionwasemployedtoarrangein14lines.Theprogressionrateoftheoptotypesizebetweentwolineswas1.2589andthetestingdistancewas3m.VisualacuityscorecouldberecordedaslogMARnotationordecimalnotation.Agestratifieddiagnosticcriteriaforamblyopiaestablishedbyconsensusstatementondiagnosisofamblyopia(2011)amongmembersoftheStrabismusandPediatricOphthalmologyGroup,OphthalmologySociety,ChineseMedicalAssociation(SPOGOSCMA)wereillustratedinthenewvisualacuitychart.RESULTS:Whenassessingvisualacuityinchildrenaged3-5yearsold,thisnewvisualacuitychartthatconsistsoffoursymmetricalshapes(triangle,square,cross,andcircle)overcameaninabilitytorecognizethelettersofthealphabetanddifficultiesindesignatingthedirectionofblackabstractsymbolssuchasthetumbling’E’orLandolt’C’,whichthesubjectswerepronetoloseinterestin.Thevisualacuityscoremayberecordedindifferentnotations:decimalacuityandlogMAR.Thesetwonotationscanbeeasilyconvertedeachotherintheneweyechart.Themeasurementsofthisnewchartnotonlyshowedasignificantcorrelationandagoodconsistencywiththeinternationalstandardlogarithmicvisualacuitychart(r=0.932,P<0.01),butalsoindicatedahightest-retestreliability(89%ofretestscoreswerewithin0.1logMARunitsoftheinitialtestscore).CONCLUSION:Theresultsofthisstudysupportthevalidityandreliabilityofdistancevisualacuitymeasurementsusingtheneweyechartinchildrenaged3to5yearsoverawiderangeofvisualacuities,andtheneweyechartisgreatforearl