简介:前列腺癌症(PCa)在外部地区(PZ)主要发生,是众所周知的,而良性的prostatic增生(BPH)典型地在转变地区发展。识别位于带的差别下面的可能的机制,我们比较了房间面对性荷尔蒙在一根PCa上皮的房间线(PC3)上从外部地区(PZsc)和转变地区(TZsc)导出的前列腺stromal的效果。首先,当房间与dihydrotestosterone(DHT)和β被对待时,我们观察到雄激素受体(AR)mRNA更高度比TZsc在PZsc被表示;-oestradiol(E2)(P<;0.05)。由ELISA,我们从PZsc和TZsc在肽生长因素的分泌物寻找了差别。我们发现那keratinocyte生长因素(KGF)分泌物与增加DHT的集中增加了(P<;0.01)并且比TZsc在PZsc是更高的。在有DHT正E2的治疗下面,PZsc分泌了更多的转变生长factor-β;1(TGF-β;1)与TZsc,而是这个模式被颠倒房间什么时候仅仅与E2被对待比。与增加DHT的集中,像胰岛素的生长factor-1(IGF-1)分泌物在PZsc增加了,但是在TZsc减少了。为了推进,在PC3房间上描绘PZsc和TZsc的效果,我们开发了一个coculture模型并且执行了MTT试金,西方的污点分析和即时RT-PCR。我们发现PZsc比TZsc更好支持了PC3房间增长和前进,特别地当与10 对待时;加10 的nmoll−1DHT;nmoll−1E2。在结论,我们的数据建议PZsc可以有一个更大的能力经由性荷尔蒙调整的生长因素比TZsc导致PCa开发和前进。这些调查结果提供在前列腺疾病位于带的差别下面的可能的机制,它可以为PCa为新奇治疗学的目标帮助搜索。
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简介:瞄准:探索17β-雌二醇的反肿瘤行动是否被homeodomain抄写因素Nkx3.1的重新表示在PC3人的前列腺癌症cells.Methods提高:PC3房间稳定地有pcDNA3.1-Nkx3.1-His向量的transfected,它带人的Nkx3.1的afull长度cDNA。稳定地,有向量pcDNA3.1的transfected作为控制被设置的PC3房间。在房间的Nkx3.1蛋白质的表示被西方的污点分析证实。PC3房间的房间增长上的Nkx3.1的Theeffect与MTT试金被检验。17β-雌二醇的Theantiproliferative和apoptotic效果被使用MTT生长测试和流动cytometric分析独自或在有Nkx3.1的联合在PC3房间上估计。表示ofapoptosis相关的蛋白质用西方的弄污被分析。结果:带Nkx3.1gene的原生质标志在PC3房间导致了Nkx3.1蛋白质的高表示。外长的Nkx3.1did的重新表示不在细胞的增长引起重要减小,而Nkx3.1enhanced的表示在PC3房间的17β-雌二醇anti-proliferative效果。Nkx3.1表示promoted17β-PC3房间的导致estradiol的apoptosis由Bcl-2的分析出现Bax,Caspase-3andpoly(自动数据处理核糖)聚合酶表示。结论:现在的学习证明Nkx3.1的thatre表示在PC3人的前列腺癌症房间提高17β-雌二醇反肿瘤行动。在试管内学习建议Nkx3.1的重新表示与雌激素反肿瘤治疗作为雄激素独立人士前列腺癌症的辅佐疗法值得进一步的考虑。
简介:TiO2nanoparticleswithdifferentphasesarepreparedbyhydrolysisoftitaniumtetrabutoxideinthepresenceofHCl.ThecompositionandmicrostructureoftheresultingsamplesarestudiedbyXRDandTEM.TheseresultsshowthattherangeofparticlesizeofTiO2isfrom20to30nm.ThemechanismofTiO2photocatalysisreactionhasbeendiscussedextensively.PhotocatalyticactivitiesofnanometerTiO2arealsoevaluatedbydegradationofthecrystalvioletsolution.ExperimentalresultsindicatethatthesynergisticactionofH2O2andultrasonicwavegreatlyenhancesphoto-catalyticreactionofTiO2.
简介:Ascientifichypothesisisproposedandpreliminarilyverifiedinthispaper:underthedrivingofseepageflows,theremightbeaverticalmigrationoffine-grainedsoilparticlesfrominteriortosurfaceofseabed,whichisdefinedas‘sub-bottomsedimentpumpaction’inthispaper.FieldexperimentswereperformedtwiceontheintertidalflatoftheYellowRiverdeltatostudythisprocessviabothtrappingthepumpedmaterialsandrecordingtheporepressuresinthesubstrate.Experimentalresultsarequiteinterestingaswedidobserveyellowslurrywhichismainlycomposedoffine-grainedsoilparticlesappearingontheseabedsurface;seepagegradientswerealsodetectedintheintertidalflat,undertheactionoftidesandsmallwindwaves.Preliminaryconclusionsarethat‘sedimentpump’occurswhenseepageforceexceedsacertainthreshold:firstly,itisbigenoughtodisconnectthesoilparticlesfromthesoilskeleton;secondly,thedegreeofseabedfluidizationorbioturbationisbigenoughtoprovidepreferredpathsforthedetachedmaterialstomigrateupwards.Thentheywouldbefirstlypumpedfrominteriortothesurfaceofseabedandtheneasilyre-suspendedintooverlyingwatercolumn.Influentialfactorsof‘sedimentpump’aredeterminedashydrodynamics(waveenergy),degreeofconsolidation,indexofbioturbation(permeability)andcontentoffine-grainedmaterials(sedimentaryage).Thisnewperspectiveof‘sedimentpump’mayprovidesomeimplicationsforthemechanisminterpretationofseveraluncleargeologicalphenomenaintheYellowRiverdeltaarea.
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简介:ThermodynamicmodelsofcalculatingmassactionconcentrationsforstructuralunitsorioncouplesinRbCl-H2ObinaryandRbCl-RbNO3-H2Oternarystrongelectrolyteaqueoussolutionsweredevelopedbasedontheionandmoleculecoexistencetheoryat298.15K.Atransformationcoefficientisneededtocomparethecalculatedmassactionconcentrationandthereportedactivitybecausetheyareobtainedatdifferentstandardstatesandconcentrationunits.TheresultsshowthatthetransformationcoefficientsbetweenthecalculatedmassactionconcentrationsandthereportedactivitiesofthesamestructuralunitsorioncouplesinRbCl-H2ObinaryandRbCl-RbNO3-H2Oternarystrongelectrolyteaqueoussolutionschangeinaverynarrowrange.ThetransformedmassactionconcentrationsofstructuralunitsorioncouplesinRbCl-H2Obinarysystemareingoodagreementwiththereportedactivities.ThetransformedmassactionconcentrationsofRbClandRbNO3inRbCl-RbNO3-H2Oternarysolutionarealsoingoodagreementwiththereportedactivities,aRbCland3RbNOa,withdifferenttotalionicstrengthsas0.01,0.05,0.1,0.5,1.0,1.5,2.0,3.0and3.5mol/kg,respectively.AllthoseresultsmeanthedevelopedthermodynamicmodelofstrongelectrolyteaqueoussolutionscanreflectstructuralcharacteristicsofRbCl-H2ObinaryandRbCl-RbNO3-H2Oternarystrongelectrolyteaqueoussolutionsandthemassactionconcentrationalsostrictlyfollowsthemassactionlaw.