摘要
LigninservesasoneofthemostimportantmolecularfossilsfortracingTerrestrialOrganicMatters(TOMs)inmarineenvironment.Extractionandderivatizationofligninoxidationproducts(LOPs)arecrucialforaccuratequantificationoflignininmarinesediment.Herewereportamodificationoftheconventionalalkalinecupricoxide(CuO)oxidationmethod,themodificationconsistinginasolidphaseextraction(SPE)andanovelon-columnderivatizationbeingemployedforbetterefficiencyandreproducibility.Inspikingblanks,recoverieswithSPEfortheLOPsarebetween77.84%and99.57%withrelativestandarddeviations(RSDs)rangingfrom0.57%to8.04%(n=3),whilethosewithtraditionalliquid-liquidextraction(LLE)arefrom44.52%to86.16%withRSDsbeingfrom0.53%to13.14%(n=3).Moreover,thereproducibilityisgreatlyimprovedwithSPE,withlesssolventconsumptionandshorterprocessingtime.Theaverageefficiencyofon-columnderivatizationforLOPsis100.8%±0.68%,whichissignificantlyhigherthanthoseofin-vialorin-syringederivatization,thusresultinginstilllessconsumptionofderivatizingreagents.LignininthesurfacesedimentssampledfromthesouthofYangtzeRiverestuary,China,wasdeterminedwiththeestablishedmethod.Recoveriesof72.66%to85.99%withstandarddeviationlessthan0.01mg/10gdryweightareobtainedexceptforp-hydroxyben-zaldehyde.ThelignincontentΣ8(producedfrom10gdrysediment)intheresearchareaisbetween0.231and0.587mg.S/VandC/Vratios(1.028±0.433and0.192±0.066,respectively)indicatethattheTOMsinthisregionareoriginatedfromamixtureofwoodyandnonwoodyangiospermplants;thehighvaluesof(Ad/Al)vsuggestthattheTOMshasbeenhighlydegraded.
出版日期
2013年01月11日（中国期刊网平台首次上网日期，不代表论文的发表时间）
