简介：ThenewspreadingconchosporesotPorphyrayezoensis,P.haitanensisandP.katadaivar.hemiphyllaareinducedbyN-Methy-N'-Nitro-N-Nitrosoguanidine(NG),astrongmutagentoinducethemutantofchromatophore.Itisshowedthat(1)nomutantshavebeeninvestigatedinallcontrolgroup.(2)TheconchosporesofallthethreespeciesofPorphyraareeasytobeinducedbyNGfortheyareinthestageofbreeding,Inthelowestdose(10μg/mL)ofthisexperiment,themutantrateofP.yezoensisisupto57.9%,thatofP.haitanensis30.7%andthatofP.katadaivar.hemiphylla51.7%.(3)Themutantratecannotincreaseobviouslybyincreasingthereagentconcentrationorprolongingtheinductiontime.Withinthescopeofexperiment,theinducementeffectsofthreespeciesofPorphyraaremuchsimilar,andthemutantrateisnear50%.TheoptimuminductionconcentrationofNGis10μg/mL,andtheoptimuminductiontimeis30minutes.

简介：TheyoungthalliofPorphyrayezoensisandP.haitanensisareinducedbyN-Methy-N'-Nitro-N-Nitrosoguanidine(NG),astrongmutagentoinducethemutantofchromatophore.Itisshowedthatnomutantshavebeeninvestigatedinallcontrolgroups,whileintheinducedgroups,itiseasytofindmutantcellsormutantcellmassesinthethallus.ThepigmentationmutantsmaybetheresultofNGinducement.Themutantrateincreasesobviouslywiththemutagenconcentrationorbyprolongingtheinductiontime.Withinthescopeofexperiment,theinducementeffectsofPorphyrayezoensisareraisedfrom11.2%to28.7%,andthoseofP.haitanensisfrom10.1%to20.2%.Accordingtoourexperiments,theamountofmutantcellsinthesameareadoesnotincreasewiththeinductionintensityTheamountofmutantcellsineverygroupofinducementofbothspeciesofPorphyrachangesverylittlewiththefixedfieldscheckmethod.Thelowestdose(25μg/mL)ofthisexperimenthasahighmutantrate.Themutantrateshowsthepercentageofmutantcellsinallthesurvivedcells,whiletheamountofmutantcellsreflectstheeffectofinducement.ThustheoptimuminductionconcentrationofNGis25μg/mL,andtheoptimuminductiontimeis30minutes.

简介：Thegeneticdiversityanddifferentiationof6mudcrabScyllaserratapopulationsfromsoutheasternChinaareinvestigatedusingallozymeelectrophoresis.Themeannumberofallelesperlocuspopulationis1.3±0.1;thepercentageofpolymorphiclociperpopulationis27.3;theobservedheterozygosityrangesfrom(0.195±0.083)to(0.241±0.090)andtheexpectedheterozygosityrangesfrom(0.105±0.043)to(0.131±0.047).Thecoefficientofgenedifferentiationamongpopulationsislow(Fst=0.032),indicatingthatonly3.2%ofthetotalgeneticdiversitycomesfrominter-population,whiletheremaining96.8%comesfromintra-populationdifferences.Thegeneticdistanceamongpopulationsis0.000～0.008(theaverageis0.002).Geneflowamongthepopulationsislarge(Nm=7.56).Geneticstructureisverysimilaramong6Scyllaserratapopulations.

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简介：PCR-DGGEapproachwasusedtoanalyzebacterialdiversityinthebottomsectionofsevenarcticseaicesamplescolletedfromtheCanadaBasin.Thirty-two16SrDNAsequenceswereobtainedfromprominentDGGEbands.Theclosestrelativesofthesesequencesarefoundtobethoseofcultivatedorunculturedbacteriafromantarcticorarcticseaice.Phylogeneticanalysisclusteredthesesequencesorphylotypeswithinα-proteobacteria,γ-proteobacteriaandCFB(cytophaga-flexibacter-bacteroides)group.Sequencesbelongingtoγ-proteobacteriaweredominantandmembersoftheCFBgroupwerehighlyabundant.ItwassuggestedthattheCFBgroupwastherepresentativeofthebottomsectionofseaicesamples.

简介：放大碎片长度多型性(AFLP)技术被用来在马尼拉蚌(Ruditapesphilippinarum)的四张自然人口分析基因差异，在中国的四个海区域分布式，我。e.，Bohai海，Huanghai海，华东海和华南海。264AFLPloci在195individuals被分析并且揭示了基因差异的高水平。多态的loci的百分比从92.13%～96.06%并且香农“s信息索引从0.2568～0.2756。由分析分子的变化(AMOVA)，有在Qingdao和另外的三个海区域的人口之间的基因区别的高水平，这被发现。由Nei的聚合分析“spairwisedistances由地理起源组织了标本，除了Qingdao的人口。一个结论能被得出在在中国和一些不同差别的马尼拉蚌的四张自然人口有高基因的差异，这存在在之中并且在四张人口之间。结果也显示人的耕作活动将在Qingdao的人口的基因结构上有大影响。

简介：Primitivemantle-normalizedPlatinum-groupelements(PGE)concentrationpatternsfortheZhengziyanwointrusionandDashibaoFormationbasaltsareofpositiveslope,similartomostoftheworld-classmagmaticNi-Cu-PGEsulfidedeposits.CharactersofthisintrusionanditsrelatedoresandDashibaoFormationbasaltsaretheirnegativePt-anomalyandhighconcentrationofRhrelativetoPtandPd,factsbeinginterpretedtobetheresultsofcrystallizationandfractionationofPt-alloysandspinelphase-freecrystallizationhistoryforthemagma,respectively.PGEparametersoftheDashibaoFormationbasaltsareconsistentwiththegeneraltrendofthosefoundfortheZhengziyanwointrusion,andthismightinferageneticlinkbetweenthem.