简介:Anon-equilibriumchromatographicratemodelwasemployedtosimulatetheaffinitychromatographyofurokinase.Thechromatographyprocesswasdevelopedtoayieldofhighpurityproductofurokinasefromcrudematerials.Theaffinitygelusedintheprocesswaspreparedbyanepichlorohydrin-activationmethodusingepichlorohydrinactivatedSepharose4Basamatrixandp-aminobenzamidineasaligand.ThechromatographicprocesswerenumericallysimulatedandanalyzedwiththeaidofVERSE-LCcomputersimulator.Consideringthebasicprinciples,ratemodelwiththebackmixingincolumninletwasutilizedinsimulatingandstudyingtheeffectofthecolumninletpatternonotherparameters.Comparisonofthesimulationresultswiththeexperimentaldatashowedthattheratemodelcanbeusedtodescribetheaffinitychromatographyofurokinaseinafixedbedcolumnwithsatisfactoryaccuracy.