简介：Theunifiedtheoreticalmomentexpressionsforelutionchromatographyandfrontalchromatographywhenthesorptionprocessisdescribedbyalinearmodelwerederived.Themomentexpressionsderivedbypreviousauthorscanbeobtainedfromtheseunifiedtheoreticalmomentexpressions.Inthispaper,amathematicalanalysishasbeencarriedoutsoastosetupaunifiedtheoreticalbasisforelutionandfrontalchromatography.

简介：Inconsiderationoftheadsorptionofsolvent,diluentandsolutemoleculesonthesurfaceofastationaryphase,anewequationforsoluteretentioninliquidchromatographyispresented.Thisequationincludesthreeparameters:thedisplacementequilibriumconstant(Ksd)betweenthesolventanddiluentmoleculesonthesurfaceofthestationaryphase,thetotalnumber(N)ofthesolventanddiluentmoleculesreleasedfromthestationaryphaseafteronesolutemoleculebeingadsorbed,andtheparameter(I)relatedtothethermodynamicequilibriumconstantforthesoluteadsorptiononthestationaryphase.Overthewholeconcentrationrangeofthesolventinthemobilephase,theexperimentalretentiondatacanbewelldescribedbythisequation,parametersKsd,NandIcanbeobtainedbytheregressionanalysisoftheexperimentalretentiondata,andconsequentlythenumberofthesolventandthediluentmoleculesdisplacedbyonesolutemoleculefromthestationaryphasecanalsobederivedatdifferentsolventconcentrationsinthemobilephase.

简介：Anewandaninexpensiveadsorbentofchitosancoatedsilicaforimmobilizedmetalaffinitychromatography(IMAC)wasstudied.Afteradoublecoating,thechitosancoatedonsilicabeadscouldbeupto53.4mg/gsilicabeads.WhenpH>3.8,themetalligandCu^2+waschelatedonthecoatedchitosanwithaboundcapacityof14.6mg/gchitosanwithoutintroducingiminodiaceticacid(IDA).

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简介：为用离子层析在水里决定化学的氧需求(货到付款)价值的一个新方法结合了nanoTiO2-K2S2O8共存系统被描述。photocatalytic氧化系统和nanoTiO2-K2S2O8共存系统能降级水里的有机化合物。在反应答案的所有包含硫的种类最后被转变到能被传导性察觉者在离子层析决定的硫酸盐。硫酸盐的电导率的变化与货到付款价值成正比。最佳的试验性的条件和察觉的机制被讨论。申请范围是10.0300.0mg?????贃??????踖????????┟???????????釱吗？？

简介：OrganicmodifierswereeffectivebothtoextendthemigrationtimewindowandtoimprovetheseparationofveryhydrophobiccompoundsinMEKC.Aniterationmethodwasusedtodeterminethemigrationtimeofmicelles.Thequantitativerelationshipbetweenthecapacityfactork’andtheconcentrationoforganicmodifierswasderived,whichwasinvestigatedexperimentally.Thelinearsolvationenergyrelationships(LSER)methodologywasappliedtoMEKC,andgoodlinearrelationshipsbetweenInk’andsolvatochromicparametersof15soluteswereobtainedinthepresenceoforganicmodifierindifferentconcentrations,whichindicatedanewaccessinMEKCtopredictk’fromthestructuralparametersofsolutes.TheeffectofcolumntemperatureTonk’wasalsoinvestigated.

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简介：Aim:Methodforthedeterninationoferythromycinethylsuccinale(EES)byionsuppressionchromatography(ISC)wasdevelopedandtheinfluencfactorsonISCwereinvestigated.Methods:AZorbaxSB-C18columnwasusedwith0.02mol.L^-1potassiumdihydrogenphosphate-acetonitrile(45:55)asmobilephase,ThepHandproportionofthemobilephaseshowedthegreatestinfluencesonretentionandselectivity.Therefore,thepHofmobilewasadjustedto6.8,thebhestacetonitrileproportionwas55%.Thecolumntemperaturewasmaintainedat(300±0.5)℃.AcetonitrilewasusedassolventforthesamplepreparationbecauseEESismorestableinit.Theflowratewas1.2mL.min^-1andUVdetectionwasperformedat210nm.Results:Underthesechromatographicconditions,themaincomponent(erythromycinAethylsuccinate)anditsrelatedsubstanceswereseparated.Thecalibrationcurveshowedgoodlinearityovertherangeof0.1-1.0mg.mL^-1,anditscorrelationcoefficientwas0.9998.Conclusion:Themethodisverysuitablefortheanalysisoferythromycinethylsuccinate.

简介：Fourtriazoleenantiomersofdiclobutrazol(erythroform)(1),paclobutrazol(erythroform)(2),diniconazole(3)anduniconazole(4)havebeenseparatedbyhighperformanceliquidchro-matography(HPLC)onchiralstationaryphase(CSP)OA-4700.Chromatographicdataandachiralrecongnitionmodelarepresentedfortheseparationofthesepesticideenantiomers.Theinfluenceofcolumntemperatureandcompositionofmobilephasehavebeendescribed.

简介：Graphene氧化物(去)由于它的唯一的性质和潜在的应用吸引了广泛的注意。这里，我们报导调查去是的nanosheets为毛状的气体的一个静止阶段色析法(GC)分离。GO列，由一条新一步舞涂层途径制作了，308的显示出的平均McReynolds常数，建议GC静止阶段的中等极的性质。GO静止阶段与好山峰形状为不同类型的analytes完成好分离，特别为结合Hanalytes，例如白酒和胺。不同保留行为变静止从常规静止阶段的阶段可以从它和analytes的多重相互作用发源，包含结合H，dipole–dipole，–叠并且散相互作用。而且，去列与相对标准差(RSD%)显示出好分离重制度不到0.24%(n ；= ；5)在analytes的保留时间。

简介：Thestudyonretentionbehaviorinsupercriticalfluidchromatography(SFC)isnecessarytounderstandthemechanismofthevariousinteractionsinSFC.TheretentionofSFCincarboxylicacidmethylester/polymethylsiloxane/CO2systemwasstudiedsystematicallyandtheretentionbehaviorofthiskindofcompoundsundervarioustypicaloperationconditionswasdescribedusingthemethodofanalternativeunifiedtheoryofchromatographicretention.Theresultsillustratedthatexpression:Ink.=a+b/T+cp+dp/T+ep2/Tcanbeusedtodescribequantitativelytheretentionbehaviorofcarboxylicacidmethylester/polymethylsiloxane/CO2systemintherangesofreduceddensityfrom0.549to1.411.Itwasalsofoundthattheentropyofsoluteinstationaryphaseisdependentonthedensityofsupercriticalfluid(SF)undertypicaloperatingconditionsofSFC.

简介：新奇chiral为ligand交换层析(CLEC)的静止阶段(CSP1)被第一把dimethylchlorosilane用作在硅石胶化的表面上为减少的剩余silanol组盖住试剂的结束准备，然后同时作为一个chiral选购者和恐水病的辛基组把l职业人员介绍给硅石胶化表面。CSP1上的14dl氨基的酸的enantioseparations被完成，enantioselectivity和在0.8和6.3之间的决定R从1.09～2.44。有用参考方法准备的结合的阶段(CSP2)的CSP1的色析法的表演被比较。结果证明chiral的列效率和决定R静止阶段能被使用上述修改方法改进。

简介：Thetetravalentmetalsaltsofmonoalkylphosphates[M(O3POR)2]areanewkindofstationaryphasesofChromatography-homogeneousbondedphases.Thispaperdealswiththeapplicationofceriumbis(monomyristylphosphate)assupporttoreversedphaseliquidchromatography.TheresultsshowthatthebestmobilphaseisCH3CN:H2O=95:5.Thegoodseparationtothemixturecontainingsixaromatichydrocarbonsandthedeterminationofnaphthaleneinagroupsampleshavebeenachieved.Theregressionanalysisshowsthatdetectlimits,linearitiesandprecisionforsixaromatichydrocarbonsaregood.

简介：Theoriginofpseudopeakwasstudiedbymeansofmicellarelectrokineticcapillarychromatographywithcetyltrimethylaminiumbromideasthepseudostationaryphase.Ithasbeenpointedthattwopeaksmayappearforonecomponentundercertainconditions.Experimentsshowedthattherelativeareasofthetwopeaksofanalytedependedonthetimeandthetemperatureofreactionbetweenanalyteandsurfactant,andtheconcentrationofsurfactantinthesamplesolution.Itmeansthattheinteractionbetweentheanalyteandthesurfactantisaslowprocess,andastablesubstancecanbeproducedfromtheinteraction.Itisthesubstanceandtheanalytethatmayleadtotheformationoftwopeaks.Thefastinteractionmechanismbetweenthesoluteandthemicellarshouldbequeriedfromtheexperimentresult.

简介：FractionsofplasmaproteinofmaleKunmingmice(bodyweight24.2±0.3g)，treatedwithCisplatini.p.injectionindoseof10mg/kg,wereobtainedbyseparationonSephadex-G-50columns,bufferedwithammoniumacetatetopH5.7,TheXSRFexperimentswereperformedattheBEPC(BeijingElectronPositronCollider)synchrotronradiationfacility.Theelements(Pt,S,Ca,Fe,Ni,Cu,Zn,Se,BrandSr)inthefractionoftheplasmaproteins(>22KD)wereassayedusinghighlysensitiveSXRF.TherelativeconcentrationsofelementswerecalculatedbyanormalizationofCOmptonscatteringintensityaround22KeV,afterthenormalizationforcollectingtimeofX-rayspectrumandthecountingoftheionchamber,andsubractingthecontributionofthepolycarbonatefilmusedforsupportingthesamples.ThedeterminationcouldprovethattheelementPtinplasmawasboundwithmacro-molecularprotein.CuandSwerepresentinthefractionoftheproteininmicetreatedwithCisplatinandexhibitedanincrease,therationoftreated/controlwere1.66±0.06and1.78±0.33repectively,whereasZndecreasedtoaratioof0.78±0.09,OurresultsareinagreementwithotherswhichshowedthatCisplatinexposureleadstoamarkedlossofkidneycopper,andamoderateriseindidneyzinc.However,thisworkmainlyfocussedontheimplementationofthisanalyticalprocedure,butnotontheresultsoftheinvestigationsoftheeffectofCisplatinontraceelementsinplasmaprotein.

简介：为在Glycyrrhizae，Notoginseng和人参决定五saponins(notoginsenosideR1，人参皂甙Rg1，人参皂甙Re，人参皂甙Rb1和铵glycyrrhizinate)建立一个方法，有二极管数组察觉者(HPLC爸爸)的高效液体层析方法被用于InertsilODS-SP列(4.6公里敲琠慨?湯?牰'要梥

简介：用高速度的逆流层析的围单人赛的碳nanotubes的新色析法的纯化被报导。纯化根据与钠dodecyl硫酸盐驱散围单人赛的碳nanotubes的实验被完成，并且结果混合用由n-butanol/water=1/1(v/v)组成的二个阶段系统被分开。分开的SWNT的尺寸被扫描电子显微镜学观察。结果证明高速度的逆流层析为围单人赛的碳nanotubes的纯化拥有了好efficency。

简介：Theparameteridentificationmodelisproposedfordeterminingthelinearadsorptionisothermsandthesoliddiffusioncoefficientsbyusingadsorptionchromatorgaphy.Axialdispersioncoefficientsisfirstlydeterminedbypulse-respondexperimenttechniquewithaninertsubstanceastracer,thentheelutioncurvesofchromatographyseparatingtheisomermannitolandsorbitolaredeterminedbythechromatographicmeasuringtechnique,andpinallytheadsorptionisothermsandthesoliddiffusioncoefficientsofmannitolandsorbitolonCa^2+resinsareestimatedbyusingthismodel.Theresultsshowthattheaxialdispersioncoefficientsincreasewithfluidvelocityincreasing,Theadsorptionequilibriumconstantsdecreasewithtemperaturerising;andthesoliddiffusioncoefficientsincreasewithtemperaturerising.Thetheoreticalelutioncurvesaregoodagreementwiththeexperimentalelutioncurvesoftheliquidadsorptionchromatographyseparatingthemannitolandthesorbitol.Themodelprovidesasimpleandreliableproceduretoestimatethekineticandthermodynamicparmetersoftheadsorption.

简介：从有有势力反的马尾藻类海草tenerrimum(圣)的乙醇摘录的多酚过敏效果被学习通过列层析优化分离过程。三的吸附和解吸附作用特征广泛地使用了吸附物：宏多孔的树脂，硅石胶化，和polyvinylpolypyrrolidone(PVPP)，非常分别地被评估并且为多酚的准备分离的优化学习了。这些吸附物上的静态的操作显示出多孔的树脂有的那个宏在三吸附物之中的最好的吸附和解吸附作用能力。有包装的列也是的多孔的树脂进行了优化参数例如的宏的动态吸附和解吸附作用：与在方括号显示出的最佳的价值，摘录答案的集中(4次冲淡了)，pH价值(67)，吸附速度(3BVh−1,床volumes/per小时)，乙醇(80%)的集中，elution速度(3BVh−1)和elution体积(7BV)。那么优化的色析法的过程从粗略的多酚给了62.43%的纯净，提供简历的大规模准备的一个有希望的基础在在测试上面的进一步的可伸缩之上的活跃多酚。

简介：Asimplemodelofchromatographicmechanicalmechanismispresent,andthenascricsoftheoreticalchromatographicequationsandfundamentalFormulaearederived.Thesetheoreticalequationsandformulaenotonlyreservethermodynamiccharacteristicsinthecurrentfundamentalchromatographicformulae,butalsointroduceoneormorekineticparameter,soitispossibletomakethemacroscopic-controlontheeffectofkineticcharacteristicsonchromatographicsystem.