简介：客观：为了调查与外部fixator在处理期间测量主要axials火车的不同方法并且发现，合适的压缩在一个早阶段由外部fixator装载了。方法：十八只健康大耳朵的兔子随机根据不同测量方法被划分成二个组：组织A和组B。在组A，一个紧张计量器与502胶水在外部胫骨的外皮被加，并且在组B，一个骨头cement-coatedstrain计量器在内部胫骨的外皮上被安装。组A和B被划分成二亚群A1，A2和B1，B2，分别地根据一半并且与身体重量一样的压力。Z塑造左中间柄的胫骨的截骨术被externalfixator执行并且修理。结果：组A的sealer曲线在早阶段期间戏剧性地变化了。当后者紧张价值比前者高时，内部、外部的外皮的Thetrendlines在到达稳定的阶段以后一致地去了。到达最稳定的年龄的组B的时间是短的，但是它的绝对紧张价值是不到组A的。在他们被贴在稳定的阶段以前，当亚群B1和B2的结果是一样的时，亚群A1的拒绝的速度比SubgroupA2的慢慢地是更多。在它衰退了以后，当时，组A有一个上升趋势组Bdidn“t。在他们到达了稳定的阶段，两亚群A1和A2had以后一个衰退趋势当亚群A2比亚群A1快速是更多时，当亚群B2波动时，亚群B1在明确的水平被保留。结论：在外部fixatorcan下面的轴的紧张被骨头水泥测量在vivo的涂紧张的计量器。数据可以建议身体重量负担的那一半对外部fixator合适。

简介：几乎，用非自体同源的房间，织物或机关移植的每试验性的治疗策略在临床的翻译前在小、大的动物模型被测试。因为这些策略在大多数情况中要求免疫力的抑制，抑制免疫力的协议是在移植实验的一个关键元素。然而，没有详细知识，标准抑制免疫力的协议经常在特别试验性的背景以内并且在选择模型种类关于他们的功效被使用。如此的协议的优化对到人的病人的试验性的结果的翻译恰当，因此，保证推进调查。这评论与种类特定的药metabolization和副作用的考虑关于抑制免疫力的药类以及他们的剂量和应用程序政体总结当前的知识。它也总结新奇immunomodulatory策略的当代的知识，例如间充质的干细胞或抗体的使用。因此，这评论被打算用作一个最先进的概要让研究人员精制应用试验性的免疫力的抑制和immunomodulation策略提高现出症状之前的潜的移植研究的预兆的价值。

简介：Danshensu[3-(3,4-dihydroxyphenyl)lacticacid,DSS],oneofthesignificantcardioprotectivecomponents,isextractedfromtherootofSalviamiltiorrhiza.Inthepresentstudy,anesterprodrugofDanshensu(DSS),palmitoylDanshensu(PDSS),wassynthesizedwiththeaimtoimproveitsoralbioavailabilityandprolongitshalf-life.TheinvitroexperimentswerecarriedouttoevaluatethephysicochemicalpropertiesandstabilityofPDSS.AlthoughthesolubilityofPDSSinwaterwasonly0.055mg·mL~(-1),itssolubilityinFaSSIFandFeSSIFreached4.68and9.08mg·mL~(-1),respectively.Octanol-waterpartitioncoefficient(logP)wasincreasedfrom-2.48ofDSSto1.90ofPDSS.PDSSwasrelativelystableintheaqueoussolutioninpHrangefrom5.6to7.4.Furthermore,thepharmacokineticsinratswasevaluatedafteroraladministrationofPDSSandDSS.AUCandt1/2ofPDSSwereenhancedupto9.8-foldand2.2-fold,respectively,comparedtothatofDSS.Cmaxwas1.67±0.11μg·mL~(-1)forPDSSand0.81±0.06μg·mL-1forDSS.Thus,theseresultsdemonstratedthatPDSShadmuchhigheroralbioavailabilityandlongercirculationtimethanitsparentdrug.

简介：Thecalciumchannelblocker,verapamil,hasbeenshowntoreducescarformationbyinhibitingfibroblastadhesionandproliferationinvitro.Itwasnotclearwhethertopicalapplicationofverapamilaftersurgicalrepairofthenerveinvivocouldinhibittheformationofexcessivescartissue.Inthisstudy,therightsciaticnerveofadultSprague-DawleyratswastransectedandsuturedwithNo.10-0suture.Thestomawaswrappedwithgelfoamsoakedwithverapamilsolutionfor4weeks.Comparedwiththecontrolgroup(stomawrappedwithgelfoamsoakedwithphysiologicalsaline),theverapamilapplicationinhibitedthesecretionofextracellularmatrixfromfibroblastsinvivo,suppressedtypeIandIIIcollagensecretionandincreasedthetotalnumberofaxonsandthenumberofmyelinatedaxons.Thesefindingssuggestthatverapamilcouldreducetheformationofscartissueandpromoteaxongrowthafterperipheralnerverepair.

简介：ActivinA，转变生长因素贝它(TGF-)的一个多功能的因素总科，被microglia和巨噬细胞主要生产，并且它的反煽动性并且支持inflammatory活动是与巨噬细胞功能有关的两个。然而，在vivo的剩余的巨噬细胞上的activinA的直接效果仍然保持不清楚。在现在的学习，结果显示出那activinA不仅不增加了并且IL-1版本，而且在vitro并且在vivo的鼠标腹巨噬细胞的支持的吞噬细胞的能力，而它没影响MHC我和MHCII表示。而且，我们显著地发现那activinAupregulatedCD14和CD68的表情，成熟巨噬细胞的标记，在在vitro并且在vivo的巨噬细胞的表面上。这些数据建议activinA能在vitro并且在vivo导致主要巨噬细胞成熟，但是不能经由调整涉及抗原的表示的MHC分子的表示触发获得的有免疫力的反应。

简介：Objective:TheaimofthisexperimentistostudytheeffectofthreemethodsofsurfacemodificationonthecorrosionresistanceofcommercialpureTitaniumwhenusedinoralenvironmentforhalfayear.Method:48specimensofpuretitaniumweremadeanddividedintofourgroupsrandomly,onegroupwasselectedrandomlyasGroupⅠ(controlgroup),theotherthreegroupsweretreatedbythreemethodsofsurfacemodificationindividually,GroupⅡ:heatingoxidationinair(400℃,30min.),GroupⅢ:anodization(45volts,10min.),GroupⅣ:TiNcoating(firingtemperature200℃,totalcoatingtime62min.).Sixedentulousvolunteerswithhealthyoralmucosaparticipatedintheinvivostudy.Onetestingpiecefromeachgroupwasselectedandfixedinthepolishedsurfaceofuppercompletedentures.Dynamicpolarizationcurvesweretracedwithelectrochemicalmethodafterthespecimenswereplacedeitherinoralcavityorinairfor6months.Results:Afterallspecimenswereused,Ecorralteredineverygroup,Ecorrfromhightolowwereinturn:TiNcoatinggroup＞heatingoxidationgroup＞anodizationgroup＞controlgroup,noobviouspassivepotentialEpandIpwasfoundincontrolgroup.HeatingoxidationinairexhibitedsimilarEptoanodization,butIpwasremarkablylowerthanthatofanodization;TiNcoatingshowedobviouslydifferentpolarizationcurvescomparedwithheating-oxidationgroupandanodizationgroup,Ecorrwaspositive,andnoEpandIpwasfound.Conclusion:Underpresentexperimentalcondition,allthethreetreatmentmethodscouldenhancecorrosionresistanceofpuretitaniuminoralenvironment,heatingoxidationinairexhibitedbetterresistancetocorrodethananodization,TiNcoatingpossessedthemostexcellentcorrosionresistance,evenafterexposedinoralconditionfor6months,therewaslittlechangeofcorrosionresistance.ThereforeTiNcoatingcouldbeadoptedtoimprovecorrosionresistanceofpuretitaniuminoralenvironment.

简介：AIM:Toinvestigatethedamagingeffectofhigh-intensityfocusedultrasound(HIFU)oncancercellsandtheinhibitoryeffectontumorgrowth.METHODS:MurineH22hepaticcancercellsweretreatedwithHIFUatthesameintensityfordifferentlengthsoftimeandatdifferentintensitiesforthesamelengthoftimeinvitro,thedeadcancercellsweredeterminedbytrypanbluestaining.TwogroupsofcancercellstreatedwithHIFUatthelowestandhighestintensitywereinoculatedintomice.Tumormasseswereremovedandweighedafter2wk,tumorgrowthineachgroupwasconfirmedpathologically.RESULTS:ThedeathrateofcancercellstreatedwithHIFUat1000W/cm2for0.5,1,2,4,8,and12swas3.11±1.21%,13.37±2.56%,38.84±3.68%,47.22±5.76%,87.55±7.32%,and94.33±8.11%,respectively.ApositiverelationshipbetweenthedeathratesofcancerceilsandthelengthofHIFUtreatmenttimewasfound(r=0.96,P＜0.01).ThedeathrateofcancercellstreatedwithHIFUattheintensityof100,200,400,600,800,and1000W/cm2for8swas26.31±3.26%,31.00±3.87%,41.97±5.86%,72.23±8.12%,94.90±8.67%,and99.30±9.18%,respectively.ApositiverelationshipbetweenthedeathratesofcancercellsandtheintensitiesofHIFUtreatmentwasconfirmed(r=0.98,P＜0.01).ThecancercellstreatedwithHIFUat1000W/cm2for8swereinoculatedintomiceexvivo.Thetumorinhibitoryratewas90.35%comparedtothecontrol(P＜0.01).IntheexperimentalgroupinoculatedwiththecancercellstreatedwithHIFUat1000W/cm2for0.5s,thetumorinhibitoryratewas22.9%(P＜0.01).Bypathologicalexamination,tumorgrowthwasconfirmedin8outof14mice(57.14%,8/14)inoculatedwiththecancercellstreatedwithHIFUat1000W/cm2for8s,whichwassignificantlylowerthanthatinthecontrol(100%,15/15,P＜0.05).CONCLUSION:HIFUiseffectiveonkillingordamageofH22hepaticcancercellsinvitroandoninhibitingtumorgrowthinmiceexvivo.

简介：AbstractObjective:To test the feasibility of a real time miniature endoscope system for imaging the nasopharynx.Study design:Preclinical assessment on skull model and cadaver.Methods:A 3.5 mm miniature endoscope was fabricated and the image capture of the nasopharynx was investigated by positioning the miniature camera system at the posterior free edge of the vomer bone. Wireless real time transmission of the images and quality was tested in a skull model. Next, three nasopharyngeal surveillance miniature camera system were developed for possible clinical translation. Two prototypes were anchored on the nasal septum and the last prototype was designed using a patient self-administered surveillance process. These prototypes were tested for feasibility on both the phantom skull and cadaveric model. Risk assessments were also performed to assess risk, safety and validate the reliability of the material utilized for clinical translation.Results:Insertion and anchorage of the miniature surveillance endoscope prototypes at the vomer bone were feasible on all 3 prototypes. The quality of captured images was reasonable and miniaturized camera was responsive to pan at different angles so that the entire nasopharynx may be surveyed. Risk assessments on the material such as pull out test, breaking force analysis, finite element test and tensile strength test were reliable for possible clinical translation.Conclusions:Real time miniature endoscope system for surveillance of nasopharyngeal cancer is feasible. Clinical translation of this technology was possible but requires further refinement in enhancing image quality and wireless transmission of the captured images.

简介：AbstractWe analyzed variations in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome during a flight-related cluster outbreak of coronavirus disease 2019 (COVID-19) in Shenzhen, China, to explore the characteristics of SARS-CoV-2 transmission and intra-host single nucleotide variations (iSNVs) in a confined space. Thirty-three patients with COVID-19 were sampled, and 14 were resampled 3-31 days later. All 47 nasopharyngeal swabs were deep-sequenced. iSNVs and similarities in the consensus genome sequence were analyzed. Three SARS-CoV-2 variants of concern, Delta (n = 31), Beta (n = 1), and C.1.2 (n = 1), were detected among the 33 patients. The viral genome sequences from 30 Delta-positive patients had similar SNVs; 14 of these patients provided two successive samples. Overall, the 47 sequenced genomes contained 164 iSNVs. Of the 14 paired (successive) samples, the second samples (T2) contained more iSNVs (median: 3; 95% confidence interval [95% CI]: 2.77-10.22) than did the first samples (T1; median: 2; 95% CI: 1.63-3.74; Wilcoxon test, P = 0.021). 38 iSNVs were detected in T1 samples, and only seven were also detectable in T2 samples. Notably, T2 samples from two of the 14 paired samples had additional mutations than the T1 samples. The iSNVs of the SARS-CoV-2 genome exhibited rapid dynamic changes during a flight-related cluster outbreak event. Intra-host diversity increased gradually with time, and new site mutations occurred in vivo without a population transmission bottleneck. Therefore, we could not determine the generational relationship from the mutation site changes alone.

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简介：AIMTo检验light-emitting-diode(带)导致的网膜的neuronal房间损坏和它的波长驱动的病原的mechanisms.METHODSSprague-Dawley老鼠暴露于蓝色LEDs(460nm)，绿LEDs(530nm)，和红LEDs(620nm)。染色的网膜电图描述术(尔格)(H&E)，Hematoxylin和曙红，传播电子显微镜学(TEM)，标记的终端deoxynucleotidyltransferasedUTP刻痕结束(TUNEL)，和染色的immunohistochemical(IHC)，西方的弄污(WB)并且superoxide阴离子的察觉(O2桰獯桰牯汹瑡潩???歁???????????椠浭湵景畬牯獥散据??啓呌？？

简介：Thepresentresearcheffortwasaimedtodevelopcolontargeteddrugdeliverysystem(CTDDS)ofrhubarb,herbaldrugusingamixedfilmofpectinandethylcellulose(EC).Pectinandethylcelluloseweremixedinvariousproportionstocoatthecoretablettotargetcolon.Themethanolicextractofrhubarbwasusedandthedoseoftheextractineachformulationwasfinalizedbyestimatingtheemodincontentinitbyhighperformacethinlayerchromatography(HPTLC).Invitrodrugrelease,erosionstudyinpresenceandabsenceofpectinaseenzymeandreleaseconstant(K)ofzeroorderwasmeasuredforeachformulation.Theformulationwasoptimizedbyusing32fullfactorialdesign.Formulationhaving50%pectinasacoatingpolymerwith12%coatweightwasselectedasanoptimizedformulation(OF)onthebasisof%similaritywithmaximumdesirabilitybutthisformulationwasnotabletoretardthereleaseofdruginstomachandupperintestinefully.SoitwasfurthercoatedwithEudragitS100(ES)(3%coatweight).Theoptimizedformulation,coatedwithESindicatedsignificantlaxativeactivityonloperamideinducedconstipationinrats.TheresultsrevealedthatCTDDSofrhubarbusingtwocombinedapproachesofbiodegrablemicroflora-activatedsystemandpH-sensitivesystemexhibitedapromisingcolontargetingperformance.

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简介：Poly-α-aminoacidisakindofbiodegradablebiomedicalmaterial.Itisusedforthepreparationofsustaineddrug-releasedevice.Therearesomeadvantagesofthedrugdeliverydevices.Theycanprovidethetherapywithsideeffects.Thedrugre-leaseismaintainedatabeneficialrateforaprolongedtime.Thepolymermaybebiodegradedafterthedrughasbeenexhausted.

简介：ThepurposeofthisstudywastodesignabioreactorforbioarLificialliverandstudyitsefficacythroughinvitroexperiment;testifiedthesafetyandefficacyoftheBALthroughtreatmentwithBALonacutefulminantischemichepaticfailurecaninemodels.

简介：Ｔ－ＬＹＭＰＨＯＣＹＴＥＭＥＤＩＡＴＥＤＴＵＭＯＲＣＥＬＬＤＥＳＴＲＵＣＴＩＯＮＩＮＶＩＶＯＡＳＳＯＣＩＡＴＩＮＧＷＩＴＨＡＳＰＥＣＩＦＩＣＦＥＡＴＵＲＥ　ＯＦ　ＡＰＯＰＴＯＳＩＳＹｕＤａ鱼达；ＹａｎｇＨｕａ杨骅；ＺｈｅｎｇＳｈｕ郑树；ＷａｎｇＸｉａ...

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简介：AbstractBackground:Angiogenesis and hypoxia-inducible factor 1α (HIF-1α) play major roles in solid tumors. This study aimed to establish a longitudinal and multimodal imaging model for in vivo evaluation of HIF1α and angiogenesis in breast cancer.Methods:By transfection of a 5 hypoxia-responsive element (HRE)/green fluorescent protein (GFP) plasmid, the cell line Ca761-hregfp was established, which emitted green fluorescence triggered by HIF-1α under hypoxia. The cells were subjected to CoCl2-simulated hypoxia to confirm the imaging strategy. We grew Ca761-hre-gfp cells in the left rear flanks of twelve 615 mice. Experiments were conducted on days 4, 9, 15, and 19. For in vivo analysis, Ca761-hre-gfp subcutaneous allografted tumors were imaged in vivo using contrast-enhanced ultrasound (CEUS) and fluorescence imaging (FLI) during tumor development. The tumor size, CEUS peak intensity, and FLI photons were measured to evaluate tumor growth, angiogenesis, and HIF-1α activity, respectively. After each experiment, three mice were randomly sacrificed and tumor specimens were collected to examine HIF-1α activity and the microvessel density (MVD).Results:In vitro, both green fluorescence and HIF-1α expression were detected in Ca761-hre-gfp cells treated with CoCl2, indicating the suitability of the cells to detect HIF-1α activity. In vivo, HIF-1α activity first increased and then decreased, which was significantly correlated with angiogenic changes (r = 0.803, P = 0.005). These changes were confirmed by immunohistochemical staining of HIF-1α and MVD.Conclusions:The findings validated the Ca761-hre-gfp murine allograft model for reliable evaluation of HIF-1α activity and angiogenesis longitudinally using both molecular and pre-clinical non-invasive imaging modalities. The cell line may be useful for studies of anti-HIF pathway therapies.

简介：试图学习在里面vivo男白化体老鼠上的Tabernaemontanadivaricata叶摘录的反煽动性的活动。方法水的煎和甲醇叶摘录为他们减少巴豆的能力被测试在在热门申请以后的鼠标耳朵的导致油的浮肿。甲醇叶摘录dose-dependently禁止了巴豆在老鼠的导致油的耳朵浮肿(ID50<500?g

简介：Theanticoagulanteffectofleechsalivawastraditionallyemployedinthetreatmentofdiabetesmellituscomplicationssuchasperipheralvascularcomplications.Thisstudywascarriedouttoexaminetheeffectofleechsalivaextract(LSE)onbloodglucoselevelsinalloxan-induceddiabeticrats.First,LSEwascollectedfromleecheswhichwerefedonaphagostimulatorysolution.Second,totalproteinconcentrationwasestimatedusingtheBradfordassay.Third,diabeticratswereinjectedsubcutaneously(sc)withLSEatdosesof500and1000μg·kg1bodyweight(bw).Otherdiabeticratswereinjectedscwithinsulinatdosesof10and20U·kg1bw.AnothergroupwasinjectedsimultaneouslywithLSE(250μg·kg1bw)andinsulin(10U·kg1bw).Fastingbloodglucose(FBG)concentrationsweremonitoredduringastudyperiodofeighthoursatregularintervals.FindingsshowedthatbothdosesofLSEresultedinasignificantandgradualdecreaseinFBGstartingfrom10%18%downfallaftertwohoursofinjectionreachingthemaximalreductionactivityof58%aftereighthours.Remarkably,LSEwassufficienttobringtheratstoanearnorm-glycemicstate.Thehighdoseofinsulininducedaseverehypoglycemicconditionafter24hofinjection.ThelowerdosewasabletodeclineFBGfor26hinratswhichbecamediabeticagainafter8h.Ontheotherhand,theconcurrentinjectionoflowdosesofLSEandinsulinproducedahypoglycemiceffectwithallratsshowingnormalFBGlevels.Takentogether,thesefindingsindicatedthatthesubcutaneousinjectionofLSEofthemedicinalMalaysianleechwasabletoprovidebetterglycemiccontrolcomparedwithinsulin.Moreover,thesynergismbetweenLSEandinsulinsuggeststhatLSEcouldbeutilizedasanadjuvantmedicationinordertoreduceinsulindosageortoachievebettercontrolofbloodglucose.