简介:FieldstudieswereconductedintheJiuwantanForestFarm,HuaduDistrictofGuangzhouCityfromJune2004toMarch2006toevaluatetheeffectsofplantingspacings(0.5m×0.5m,0.5m×1.0mand1.0m×1.0m)anddifferentfertilizers(bio-fermentedmanure,NPKcompoundfertilizerandNPKmixedfertilizer)onthegrowthofDaemonoropsmargaritaegrownunderirrigationandfull-lightconditionsforedibleshootsproduction.Theeffectsofapplicationofdifferentfertilizersonthemeanheight,lengthofstem...
简介:MultiplicationofElaeagnusangustifoliaL.wasexaminedinvitrosuccessivelyfromasingleshootunderthespecifiedconditionofdifferentmedia,plantgrowthregulators,pHvalueandsucroseconcentration.ItwasshowedthatMMS1wasthemostsuitablemediumonshootmultiplicationamong5mediaconcerned;BAPwasthemosteffectiveoneamongallthecytokinininvolved,BAP,KN,TDZandZT;theexpluntofthetophalf-partfromashootproducedmorenewshootsthanthatofthefoothalf-partdid;morenewshoots(>2cm)wereproducedunder3%sucrosebetweentheconcentrationswithtophalf-partexplants;shootscouldgrowwellbetweenpH4.4andpH7.0,andthebiggestnumberofshootswasproducedinpH5.6,whileinpH5.8themaximumrootingrateappeared.Asaresult,thecombinationof0.5μMBAPand0.1μMIBAonMMS1mediuminducedthemaximumshootmultiplication.Thenumberofshootamplified3timesin1month,and3^12shoots(>2cm)mightbetheoreticallymultipliedannuallyfromasingleshoot.
简介:Eucalyptusisveryrecalcitranttoinvitroculture.Inthisresearch,anefficientshootorganogenesissystemwasdevelopedusing60-day-oldplantsofEucalyptusglobulusgrowninvitroandnon-aeratedliquidmediumtoimproveshootproliferation.Cultureswereinitiatedwithhypocotylsandleafsegmentsfromplantletscultivatedonsemisolid1/2MSmodifiedmediumsupplementedwith4.44lM6-Benzyladenine(BA)and16.1lM1-Naphthaleneaceticacid(NAA).Calliweretransferredtoshootinductionmedium,witheither0.5or2.7lMNAA.Shootmultiplicationwascarriedouton4.44lMBA+0.5lMNAAmedium,andsemisolidandnon-aeratedliquidsystemswerecomparedforimprovingshootproliferation.RootingofadventitiousshootswasevaluatedonmediumcontainingNAAorIndole-3-butyricacid-IBA(5and16lM).Callogenesiswasobtainedfrombothtypesofexplants,althoughshootformationwasonlyobtainedfromleaf-derivedcalli.Shootproliferationon4.44lMBA+0.5lMNAAresultedinthemostshoots/callus.Non-aeratedliquidmediumwasmoreefficientinpromotingshootmultiplication(53.5shoots/callus)thanwassemisolidmedium(28.5shoots/callus).Levelsofphenoliccompoundsweresignificantlyreducedintheshootscultivatedinliquidmedium.Efficientrooting(76%)wasobtainedusing16lMIBA.
简介:Thehighgrowth-stimulatingeffectofplantextracthasurgedtheplantbiotechnologiststousenaturalsupplementsintheculturemediainsteadofsyntheticphytohormones.Weadvocatedtheeffectofsproutedsorghumextract(SSE)onemergence,invitroacclimatization,andgeneticfidelityincoleoptilederivedcallusofindicaricevarietyADT36.TheuseofSSEwithMurashigeSkoogmediumefficientlyacclimatizedtherootandshootapicalsystems.Ahighermatandseminalroots(3.4gbiomass)withanefficientshootprimordiumelongationwereobservedwithanincreaseintheconcentrationofSSE.SeedstreatedwithSSEmediumshowedhighergerminationandearliercoleoptilematurationabout48hcomparedtountreatedseeds,andtherewasahigherexpressionofeEF-1αwithanincreaseincoleoptilelength.B5mediumwaseffectiveoninducingembryogenicandnodularcallusfrom3-day-oldcoleoptilewith3.0mg/L2,4-dichlorophenoxyaceticacidandfurtherproliferatedeffectivelywith0.8mg/Lkinetinwithafreshweightof180mg.Highlysignificantregenerationwasobservedwithcombinationof2.5mg/L6-benzylaminopurineand3.0mg/Lα-naphthaleneaceticacid.Themetabolicandgeneticprofilesofinvitroanddirectlycultivatedplantswerethesame,examinedthroughFourier-transforminfraredspectroscopy,randomamplifiedpolymorphicDNA(RAPD),inter-simplesequencerepeat(ISSR)andR-ISSR(combinationofRAPDandISSR)markers,respectively,andthusconfirmingthesignificantefficacyoftheSSEincorporatedmedium.DisarmedT-DNAwastransformedtocoleoptilederivedcallusthroughAgrobacteriumtumefaciensLBA4404andconfirmedbyGUSassay.TheT-DNAintegrationwasconfirmedbyDNAblotanalysisusingDNAfromtransientGUS-expressedexplants.Thus,SSEcanbeusedasanaturalandorganicsupplementfororganogenesisandefficientacclimatizationsofshootandrootapicalmeristemsinregeneratedplants.
简介:Sincethegenerationoffull-sibartificialtriploidfamilies,rapidcloneestablishmentandgeneticimprovementshavebeenneeded.Here,wereportaninvitromethodofdirectshootregenerationofatriploidhybridpoplar[(Populussimonii9P.nigra‘Italica’)9(P.9‘popularis’)].Usingdifferentrandomizedblockdesigns,weselectedonetriploidtoevaluatetheexplanttype,optimalconcentrationsofplantgrowthregulatorsandagar,andculturetimeunderlightordarkconditionsover60days.Thehighestrateofshootinduction,80.0%,wasobtainedusingMurashigeandSkoog(MS)mediumsupplementedwith0.2mg/Lbenzyladenine,0.04mg/Lnaphthaleneaceticacid(NAA),and5.5g/Lagarforthefirst30daysinthedark,then3g/Lagarforthenext30daysinlight.Thislastmediumyieldedthebestrateofshootinduction(6.32shoots/explant).Thesethreemediawerealsousedtoevaluatetheinfluenceofthegenotypesoftheparentsandhybridtriploidsonregeneration.Twoparentsandthreeofthefourfull-sibtriploidswereregeneratedsuccessfully;differentgenotypesandexplanttypessignificantlyaffectedtherateofshootinductionandaveragenumberofshoots.Leavesbutnotpetioleswereasuitableexplant.Onegenotypeproducedthehighestrateofshootinductionof96.67%.Half-strengthMSmediumsupplementedwith0.2mg/Lindolebutyricacidand0.04mg/LNAAwasthemosteffectiveforrooting;rootingratewas96.67%,survivalrateoftransplantswas73.33%,androotingfrequencysurpassed85%foreachgenotype.Overall,thisinvitroregenerationsystemwillbeusefulforthepropagationandgeneticmodificationoftriploidpoplars.
简介:Populus种类是为工业并且在生物、农业的系统上的科学学习的重要资源。我们的目的是在喜玛拉雅的白杨提高植物新生的频率(Populusciliata墙。前Royle)。TDZ的效果独自并且在有腺嘌和NAA的联合在叶柄将生物的活组织移植于培养基中培养的新生潜力上被学习。将生物的活组织移植于培养基中培养从在温室种的喜玛拉雅的白杨植物被切除。在表面消毒以后,将生物的活组织移植于培养基中培养在射击正式就职媒介上是有教养的。高百分比射击新生(86?%)在与0.004补充的MS媒介上被记录?mgL1TDZ并且79.7?mgL1腺嘌。为延伸和增加的改革射击被转移到MS?+?0.5?mgL1BAP?+?0.2?mgL1IAA?+?0.3?mgL1GA3。从在vitro开发的射击的根新生在与0.10补充的MS媒介上被观察?mg?L1IBA。喜玛拉雅的白杨小植物能在2以内被生产?在在沙和土壤的无菌的混合物的acclimatization以后的月。我们从P的叶柄将生物的活组织移植于培养基中培养开发了一个高效率植物新生协议。ciliata。