简介:Phenologyshiftsandrangeexpansionscauseorganismstoexperiencenoveldaylength-temperaturecorrelations.Dependingonthetemporalniche,organismsmaybenefitorsufferfromchangesindaylength,thuspotentiallyaffectingphenologicaladaptation.WeassessedtheimpactofdaylengthchangesonlarvaeofChrysoperlacarnea(Stephens)andEpisyrphusbalteatus(DeGeer),bothofwhichpreyonaphids.LarvaeofE.balteatusarenight-active,whereasthoseofC.carneaappeartobecrepuscular.Wesubjectedbothspeciesinclimatechamberstodaylengthsof16:8L:Dand,tocircumventdiapauseresponses,20:4L:D.Werecordeddevelopmenttimesandpredationratesofbothspecies.E.balteatusgrew13%fasterinthe16:8L:Dtreatmentandpreyedonsignificantlymoreaphids.Incontrast,C.carneagrew13%fasterinthe20:4L:Dtreatmentandhigherpredationratesin20:4L:Dweremarginallysignificant.Ourresultsshowthatdaylengthaffectsdevelopmentandpredation,butthatthedirectiondependsonspecies.Suchdifferencesintheuseofdaylengthmayaltertheefficiencyofbiocontrolagentsinachangingclimate.
简介:土豆psyllid,Bactericera(=Paratrioza)cockerelli(ulc)(半翅类:Triozidae),是土豆的一个主要害虫。研究被进行决定男性和女性在到达繁殖成熟和年龄的效果并且在性别吸引力上白天预定的年龄。成年B。cockerelli活动范围在48h以内的繁殖成熟羽化以后,与在1天在羽化和男性的日子是成熟的女性一起羽化以后。Oviposition通常在交配以后开始了2天,但是当女性们在2以内交配了天时,被推迟羽化以后。在实验室olfactometer试金,当气味来源和到这些气味的男性assayed的年龄没影响性别吸引力,女性的年龄使用了,与两个更年轻(14-day-old)并且更旧(810-day-old)男性们正在被吸引到女性,不管女年龄(14-day-old或810-day-old)。男性assayed到在不同时间的实时女性在光照发育阶段期间(在8:00和20:00小时之间)被吸引到在11:00和17:00小时之间的女性,与B在性别吸引力显示出时间的频率。cockerelli至少在光照发育阶段期间。
简介:Humanμ-opioidreceptor(HμOR)withatagofsixconsecutivehistidinesatitscarboxylterminushasbeenexpressedinrecombinantbaculovirusinfectedSf9insectcells.Themaximalbindingcapacityforthe[^3H]diprenorphineand[^3H]ohmefentanyl(Ohm)were9.1±0.7and6.52±0.23nmol/gprotein,respectively.The[^3H]diprenorphineor[^3H]OhmbindingtothereceptorexpressedinSf9cellswasstronglyinhibitedbyμ-selectiveagonists[D-Ala^2,N-methyl-Phe^4,glyol^5]enkephalin(DAGO),Ohm,andmorphine,butneitherbyδnorbyκselectiveagonist.Na^+(100mM)andGTP(50μM)couldreduceHμORagonistsetorphineandOhmaffinitybindingtotheoverexpressedHμOR.μ-selectiveagonistsDAGOandOhmeffectivelystimulated[^35S]GTPγSbinding(EC50=2.7nMand6.9nM)andinhibitedforskolin-stimulatedcAMPaccumulation(IC50=0.9nMand0.3nM).Theagonist-dependenteffectscouldbeblockedbyopioidantagonistnaloxoneorbypretreatmentofcellswithpertussistoxin(PTX).TheseresultsdemonstratedthatHμORoverexpressedinSf9insectcellsfunctionallycoupledtoendogenousCi/oproteins.
简介:Plasmamembrane(PM)Ca^2+-ATPaseactivityinpoplarapicalbudmeristematiccellsduringshort-day(SD)-induceddormancydevelopmentwasexaminedbyaceriumprecipitationEM-cytochemicalmethod.Ca^2+-ATPaseactivity,indicatedbythestatusofceriumphosphateprecipitatedgrains,waslocalizedmainlyontheinteriorface(cytoplasmicside)ofthePMwhenplantsweregrownunderlongdaysandreachedadeepdormancy.Afewreactionproductswerealsoobservedonthenuclearenvelope.Whenplantbudsweredevelopingdormancyafter28to42dofSDexposure,almostnoreactionproductswerepresentontheinteriorfaceofthePM.Incontrast,alargenumberofceriumphosphateprecipitatedgrainsweredistributedontheexteriorfaceofthePM.After70dofSDexposure,whenbudshaddevelopedadeepdormancy,thereactionproductsofCa^2+-ATPaseactivityagainappearedontheinteriorfaceofthePM.TheresultsseemedsuggestingthattwokindsofCa^2+-ATPasesmaybepresentonthePMduringtheSD-induceddormancyinpoplar.OneistheCa^2+-pumpingATPase,whichislocatedontheinteriorfaceofthePM,formaintainingandrestoringtheCa^2+homeostasis.Theothermightbeandecto-Ca^2+-ATPase,whichislocatedontheexteriorfaceofthePM,fortheexocytosisofcellwallmaterialsassuggestedbythefactofthecellwallthickeningduringthedormancydevelopmentinpoplar.