简介：Theglass-formingability(GFA)andmagneticpropertiesoftheGd_(50)Coso-basedamorphousaiioywithAladditionsubstitutionforCoareinvestigated.ItisfoundthattheGFAandmagneto-caloriceffectoftheGd_(50)Co_(45)Al_5amorphousaiioyarebetterthanGd_(50)Co_(50)amorphousalloy.Themaximummagneticentropychange(-△S_m~(peak))andthemagneticrefrigerantcapacityoftheamorphousalloyunderafieldof5Tareabout6.64J·kg~(-1)K~(-1)and764J·kg~(-1),respectively.Thefielddependenceofmagneticentropychangemeetstheonepredictedbythemeanfieldtheory,whichisinvestigatedforabetterunderstandingofthemagneto-caloricbehaviorsoftheGd_(50)Co_(45)Al_5amorphousalloy.

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简介：摘要：本文章运用思辨的思维，探索生物计算的能力在城市设计中的应用。多项科学研究已经证实了多头绒泡菌的智慧和其生物计算能力，这种单细胞生物及其生物生长规律现象揭示了其计算和设计城市网络的可能性。本研究是基于多头绒泡菌的生长以及它的集体智能特性成为设计工具的途径。在没有大脑但有记忆有机体的情况下，我们收集了多头绒泡菌这个单细胞生物的分布式记忆，并假设它可以成为重新定义城市空间推理的替代方式。为了研究多头绒泡菌生物计算能力在城市设计中的应用，我们在生物体的不同尺度上进行了观测实验，并折射到城市和建筑尺度上来测试结果。因此，本研究试图提出一种基于生物计算方法的非常规设计新方法，作为重新定义和重组国土系统的推测机制。

简介：Bonemorphogeneticprotein(BMP)isanefficientbone-inducingproteinwhichmainlyexistsinbonematrix.TheimplantsofBovineBMP(bBMP)extractedfrombovinebonematrixcaninducedifferentiationofmesenchymalcellsofmicemusclein-tochondroandosteo-cytesandformnewbonefurther.Inordertoevaluateim-plantablebiomaterialthatpossessesnotonlyosteogenicability,butalsostengtn,

简介：EctomycorrhizalfungusLaccariabicolorS238N,isolatedfromaforestsoilincentralFrancein1990s,hasdemonstratedunequivocallyandabilitytopromotepinegrowth.Inthepresentnurserybedexperiment,theabilityofthisectomycorrhizalfungustoincreasegrowthandPandKuptakeofDouglasFirseedlings(Zone22)wasexamined.Growthofinoculatedseedlingswasovertwice(plantheight)andthreetimes(biomass)thatofnon-inoculatedones.Similarly,boththeconcentrationsandtheamountsofPandKuptakebyseedlingsweresignificantlyincreasedbyfungalinoculation,indicatingtheimprovementofPandKnutritioninmycorrhizalseedlings.Incontrast,Al-Pinthesoilswasdecreasedobviouslybyplants,especiallybymycorrhizas,suggestingutilizationofthissoilPpoolbyplantsandmoreefficientAl-Pmobilizationbymycorrhizasthanbynomycorrhizas.Moreover,Kextractedby1mol/LHClfollowingconsecutiveextractionofH2OandCH3COONH4,whichmaynotbeplantavailable,couldbeutilizedbyfunguscoloniedroots.ThiscouldbeexplainedbythereleaseofprotonsandoxalatebyhypaewhichleadstoreplacementofinterlayerKinnonexpanded2:1claymineralsandbio-weatheringofphyllosilicates.

简介：AIM:JS-38(mitothiolore),asyntheticversionofametaboliteisolatedfromXenorhabdussp.,wasevaluatedforitsanti-tumorandwhitebloodcell(WBC)elevatingactivities.METHOD:Theseanti-proliferativeactivitieswereassessedinvitrousingapaneloftencelllines.Theanti-tumoractivitiesweretestedinvivousingB16allograftmousemodelsandxenograftmodelsofA549humanlungcarcinomaandQGYhumanhepatomainnudemice.Theanti-tumorinteractionsofJS-38andcyclophosphamide(CTX)or5-fluorouracil(5-Fu)werestudiedinaS180sarcomamodelinICRmice.SpecificstimulatoryeffectsweredeterminedonperipheralneutrophilsinnormalandCTX-and5-Fu-inducedneutropenicmice.RESULTS:TheIC50valuesrangedfrom0.1to2.0μmol·L-1.JS-38(1μmol·L-1)causedanincreaseinA549tumorcellapoptosis.Multi-dailygavageofJS-38(15,30,and60mg?kg-1?d-1)inhibitedinvivotumorprogressionwithoutasignificanteffectonbodyweight.JS-38additivelyenhancedtheinvivoanti-tumoreffectsofCTXor5-Fu.JS-38increasedperipheralneutrophilcountsandneutrophilratesinnormalBALB/cmicealmostaseffectivelyasgranulocytecolony-stimulatingfactor(G-CSF).InmicewithneutropeniainducedbyCTXor5-Fu,JS-38rapidlyrestoredneutrophilcounts.CONCLUSION:TheseresultssuggestthatJS-38hasanti-tumoractivity,andalsohastheabilitytoincreaseperipheralbloodneutrophils.

简介：AbstractObjective:This study was performed to investigate the relationship between the human melanogenesis and antioxidant systems and to further confirm the synergistic effect of oxyresveratrol (OXYR) and resveratrol (RES) in human epidermal melanocyte cell line.Methods:The human epidermal melanocyte line PIG1 cells were divided into the UV groups and control group, treated with different doses of UVB and without UVB, respectively. MTT assay and flow cytometry were used to detect cell viability and apoptosis. The expression of Nrf2/HO-1 and melanogenesis-associated proteins/genes was measured by Western blotting and real-time qPCR (RT-qPCR). pCMV6-XL5-Nrf2 was used to upregulate the expression of Nrf2. Subsequently, the proteins/genes levels of Nrf2/HO-1 and tyrosinase (TYR), melanin/eumelanin content, and reactive oxygen species (ROS) were analyzed. Isobologram analysis and cell experiment were used to analyze whether OXYR and RES inhibit TYR synergistically. Western blotting, RT-qPCR, and NaOH splitting method were used to determine the Nrf2/HO-1 and melanogenesis-associated proteins/genes expression and melanin content to evaluate the efficacy of OXYR and RES.Results:The activated Nrf2 and HO-1 eliminated ROS produced by UVB irradiation. The melanogenesis-associated proteins/genes of melanocyte-inducing transcription factor (MITF, P < 0.01 on protein expression), TYR (both P < 0.01), TYR-related protein (TRP)-1 (both P < 0.05), and TRP2 (P < 0.05 on mRNA expression) were activated in PIG1 cells by UVB irradiation. Simultaneously, the upregulation of Nrf2 significantly reduced melanogenesis formation (P < 0.001) and TYR level (P < 0.01 on protein expression). Moreover, OXYR and RES synergistically inhibited TYR activity (P < 0.001) and reduced melanin content (P < 0.001).Conclusions:A microbalance exists between Nrf2/HO-1 signaling and melanogenesis production in the UVB-induced responses of melanocytes. Simultaneously, OXYR enhances the ability of RES to inhibit melanin production.

简介：AbstractBackground:The CHA2DS2–VASc score was initially applied to stratify stroke risk in patients with atrial fibrillation (AF) and was found to be effective in predicting all-cause mortality outcomes. To date, it is still unclear whether circulating long non-coding RNAs (lncRNAs) as emerging biomarkers, can improve the predictive power of the CHA2DS2–VASc score in stroke and all-cause mortality.Methods:Candidate lncRNAs were screened by searching the literature and analyzing previous RNA sequencing results. After preliminary verification in 29 patients with AF, the final selected lncRNAs were evaluated by Cox proportional hazards regression in 192 patients to determine whether their relative expression levels were associated with stroke and all-cause mortality. The c-statistic, net reclassification improvement (NRI), and integrated discrimination improvement of the patients were calculated to evaluate the discrimination and reclassification power for stroke and all-cause mortality when adding lncRNA expression levels to the CHA2DS2–VASc score model.Results:Five plasma lncRNAs associated with stroke and all-cause mortality in AF patients were selected in our screening process. Patients with elevated H19 levels were found to have a higher risk of stroke (hazard ratio [HR] 3.264, 95% confidence interval [CI]: 1.364–7.813, P = 0.008). Adding the H19 expression level to the CHA2DS2–VASc score significantly improved the discrimination and reclassification power of the CHA2DS2–VASc score for stroke in AF patients. In addition, the H19 level showed a marginally significant association with all-cause mortality (HR 2.263, 95% CI: 0.889–5.760, P = 0.087), although it appeared to have no significant improvement for the CHA2DS2–VASc model for predicting all-cause mortality.Conclusions:Plasma expression of H19 was associated with stroke risk in AF patients and improved the discriminatory power of the CHA2DS2–VASc score. Therefore, lncRNA H19 served as an emerging non-invasive biomarker for stroke risk prediction in patients with AF.

简介：Thegrowth,biochemicalcontentandbioaccumulationquantityof2,2’,4,4’-tetrabromodiphenylether(BDE-47)inSkeletonemacostatumwerestudiedunderdifferentN:Pratios(1,4,16,64and128).AllcellularbiochemicalcontentsofS.costatumpresenteddecreasingtrendovercultivationtime.Atearlystageofcultivation,thecellularprotein,carbohydrateandlipidinS.costatumpresentedhighervaluesintreatmentsofN:P=4and16.However,theywerelowerinthesetreatmentsatthelatestage,buthigherintreatmentsN:P=1and128.Similarly,BDE-47levelspercellofS.costatumwerehigherintreatmentsofN:P=4and16atearlystageofcultivation,whichwere3.8and3.7ng(106cells)-1,respectively.Atthemiddlestageofcultivation,theBDE-47levelperS.costatumcelllowered;anditfurtherreducedinthetreatmentsofN:P=4and16atthelatestagewiththevalues0.6and0.5ng(106cells)-1,respectively.However,itroseinN:P=128,reachingupto2.3ng(106cells)-1.ComparedwithBDE-47percell,BDE-47peralgalvolumeunderdifferentN:Pratiosdidnotpresentobviousdifference.ThequantityBDE-47accumulatedpercellofS.costatumwaspositivelycorrelatedwithprotein,carbohydrateandlipidpercell;meanwhile,theBDE-47pervolumehadapositivecorrelationwithbiochemicalcontentpervolume.ThevariationofbioaccumulationabilityofBDE-47inS.costatumcanbeexplainedbybiochemicalchangesduetoN:Pratios.

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简介：摘 要：开展巴林特小组可让医学生较早地认识到医患沟通的重要性和艺术性，能够及时发现自己在医患沟通中的盲点，养成自我反思的良好习惯，促进医学生换位思考培养同理心，获得医患沟通技巧指导，有助于自我效能的建立，并得到小组同伴支持有效缓解工作压力，促进医患关系改善带来的良性互动，本文从这8个方面对巴林特小组提升医患沟通能力的作用进行分析。在医学生培养方案、教学管理架构、小组组长（领导者）培训和激励机制4个方面提出有操作性的教学策略。

简介：摘要：为了探讨呼吸肌训练对自行车运动员呼吸功能及运动能力的影响，对 10 名现役男子自行车运动员进行了四周的呼吸肌训练，并于每周进行高温高湿环境下最大摄氧量（ VO 2 max ）、每分通气量（ VE ）、呼吸频率（ RR ）和运动潮气量（ Vt ）反应有氧能力、呼吸功能的指标以及主观疲劳程度（ RPE ）和最大功率数据的采集。结果显示呼吸肌训练可以提高自行车运动员在高温高湿环境下的 VO 2 max 和最大运动功率，其中 VO 2 max 在呼吸肌训练 2 周后和 4 周后均较训练前提高了约 16.1% ，最大运动功率在呼吸肌训练 4 周后提升了 30W ，提升幅度为 11.1% ；此外呼吸肌训练可以有效提高平均每分通气量（ VE ）与运动时潮气量（ Vt ）， VE 和 Vt 在呼吸肌训练 4 周后高温高湿环境下较训练前分别升高约 19.5% 和 12.5% ，训练对高温高湿环境运动的肺通气功能有明显促进作用。建议自行车等有氧耐力性运动项目可以尝试练习呼吸肌群，从而帮助提升高温高湿环境下的有氧运动能力。