简介:《中华疝和腹壁外科杂志(电子版)》为中华人民共和国国家卫生和计划生育委员会主管、中华医学会主办、首都医科大学附属北京朝阳医院承办、中华医学电子音像出版社有限责任公司出版的疝和腹壁外科专业学术电子版期刊,以疝和腹壁外科专业医师、研究人员为读者对象。本刊以电子期刊特有的表现形式,以多媒体图文声像并茂的方式,报道疝和腹壁外科领域领先的科研成果和临床诊疗经验,并报道对临床有指导作用且与临床密切结合的基础理论研究,具有很强的互动性。本刊的办刊宗旨是:贯彻党和国家的卫生工作方针、政策,贯彻理论与实践、中医与西医、普及与提高相结合的方针,反映我国疝和腹壁外科领域科研和临床工作的进展,促进国内外学术交流。
简介:Aim:ToobservetheapoptoticchangesfollowingexposuretoEMPandtoexplorethepossibleinjurymechanisminNIH-3T3fibroblasts.Methods:FollowingNIH-3T3cellswereexposedtoEMP,theproliferationandviabilityofNIH-3T3fibroblastswereestimatedbyhemacytometerandMTTMeasurements.Apoptoticratewasmeasuredbyflowcytometer.TheimnmohistochemicalSPmethodwasusedtodeterminebcl-2,p53.ThepositivelystainedcellswereanalyzedwithCMIAS-Ⅱimageanalysissystematamagnification400.AlldatawereanalyzedbySPSS8.0software.Results:TheproliferationandviabilityofNIH-3T3cellsweremarkedlyinhibitedandsignificantapoptosiswasinducedfollowingexposuretoEMP.EMPcouldincreasethenumberofnon-adherentcells,thehighestratio(33.9%)ofnon-adherentcellsalsooccurredat6h.TheAs70valueofMTTdecreasedimmediatelyat1h,6hfollowingthecellswereexposedascomparedwiththecontrol(P<0.05).Thehighestratioofapoptosiswas15.07%at6h,thendecreasedgradually.Down-regulationofbcl-2andup-regulationofp53wereinducedbyEMP(P<0.05).Conclusions:EMPcouldpromoteapoptosisofNIH-3T3fibroblasts.EMPcouldalsodown-regulatebcl-2levelandup-regulatep53levelinNIH-3T3fibroblasts.Bcl-2andp53genemaytakepartintheprocessofapoptosis.