简介:寻常狼疮是皮肤结核中最常见的类型,约占所有皮肤结核患者的半数。本病易侵犯儿童和青少年,好发于面部,影响容貌。我们用异烟肼、乙胺丁醇和利福平联合治愈寻常狼疮2例,报告如下。
简介:结合1例被误诊7年的胰高血糖素瘤综合征患者病例介绍,并分析胰高血糖素瘤罕见临床表现,该病极易误诊。但因其有反复发作的特定表现的皮损和明显异常的实验室检查,临床上根据可疑皮损,再结合糖尿病史(或血糖升高)、消瘦、贫血等临床表现应考虑该病的可能性,并进一步检查血胰高糖素及影像学定位诊断。
简介:Inordertoacknowledgethemulti-infectiondataofSTDpatientsandimprovetheprophylaxisandtherapyforSTDs,297patientsand30healthypeoplewereexaminedusingtheHSV-2plasmaantibody.TheresultsshowedthatallkindsofSTDpatientswereinfectedbyHSV-2indifierentratios.thehighestratiooccurringinsyphilispatients.
简介:Objective:TounderstandthechangeofTh1/Th2andTc1/Tc2balanceinpatientswithsecondaryearlysyphilis.Methods:PeripheralbloodThandTccellsubpopulationswereexaminedbythree-colorflowcytometryin30patientswithsecondaryearlysyphilisand20healthysubjects.Results:PercentagesofCD3^+,CD4^+TcellsandtheratioofCD4^+/CD8^+andNKcellsinsyphiliticpatientsweresignificantlylowerthaninthecontrolgroup(P<0.001),butnostatisticaldifferencewasfoundinpercentagesofCD8^+andCD19^+cellsbetweenthetwogroups(P>0.05).ThepercentageofTh1cellsandratiosofThl/Th2andTcl/Tc2insyphiliticpatientswereclearlydecreasedascomparedtothoseincontrois(P<0.001-0.01),whereaspercentagesofTc0andTc2cellsinsyphiliticswashigherthanthoseincontrols(P<0.001).Conclusion:Therecouldbeadisturbanceofcellularimmunityandtherelativelyintactfunctionofhumoralimmunityinsecondarysyphilis.ItispostulatedthatthepolarizationtowarddevelopmentofTh2andTc2cellsandtheirassociatedcytokinesmightbeinvolvedintheincompleteclearanceoftreponemesandthedevelopmentofchronicinfection.
简介:Objective:Toconstructarecombinantplasmidcontainingtheoutermembraneprotein2(Omp2)geneofChlamydiatrachomatisandexpressOmp2inE.coli.Methods:Theomp2geneofC.trachomatisserovarDwasclonedintopQE30vectorfollowingPCRamplificationfromgenomicDNA.E.coliM15transformantswereinducedtoexpressthefusionproteinbyIPTGandtheproductwasidentifiedbySDS-PAGEandWesternblot.Results:ConfirmedbyenzymecleavageanalysisandDNAsequencing,acorrectrecombinantplasmidpQE30/omp2wasconstructed.Thefusionproteinfromthetransformantswasapproximately60kDainsizeinSDS-PAGEanalysis,whichcouldspeciallyreactwithanti-6×HismousemonoclonalIgGantibodies.Conclusion:WesuccessfullyexpressedOmp2inE.coilM15,providinganefficientandsimplesystemforassayingtheimmunologicalpropertiesofOmp2.
简介:目的:探讨男性肿瘤患者化疗后勃起功能状况。方法:选取我院2011年1月至2013年12月间的50例初次接受化疗的男性肿瘤患者为研究对象,采取国际勃起功能指数-5(IIEF-5)进行评估化疗前后勃起功能状况。结果:治疗有效率为58.0%。其中,CR14例,PR15例,PD21例,SD0例。化疗前勃起功能障碍32.0%,化疗后勃起功能障碍83.0%,化疗前后勃起功能障碍发生率比较差异有统计学意义(P<0.05);化疗后消化道肿瘤、鼻咽癌、肺癌、淋巴瘤、软组织肿瘤患者IIEF-5评分较化疗前有不同程度的降低,差异有统计学意义(P<0.05)。化疗后不同病理类型间IIEF-5评分比较无统计学意义(P>0.05)。结论:临床中男性肿瘤患者实施化疗后会增加勃起功能障碍,增加其发病率。
简介:背景:汗孔角化症是一种罕见的角化性疾病,有常染色体显性遗传和后天获得两种形式。许多病例报道和系列研究都认为免疫抑制与该病发生有关。据作者所知,仅有5例骨髓移植后发生汗孔角化症的报道。结果:作者报道5例骨髓移植患者,在移植术后1~13年内诊断为Mibelli汗孔角化症。5例患者中4例的原发恶性肿瘤为白血病,第5例为非霍奇金淋巴瘤。4例白血病患者的汗孔角化症发生于缓解期,而第5例的汗孔角化症发生于淋巴瘤复发期。结论:汗孔角化症可发生于骨髓移植后。作者报道的5例是医学文献报道例数的2倍,且骨髓移植后发生汗孔角化症的发病率可能比先前公认的要高得多。由于已有报道指出皮肤癌与汗孔角化症相关,对骨髓移植受者发生的汗孔角化症进行仔细监测是十分必要的。
简介:Objective:Tostudythecellularimmunitystatusofpatientswithrecurrentgenitalherpes.Methods:Serumlevelsofinterlukin-2anditssolublereceptorandinterlukin-6weremeasuredbyELISAin34patientswithrecurrentgenitalherpes.Results:SerumlevelsofIL-2andIL-6weresignificantlydecreasedinpatientscomparedtohealthycontrols(P<0.01),andthelevelofsIL-2Rwassignificantlyincreasedinpatientswithrecurrentgenitalherpes(P<0.01).Therewerenosignificantdifferencesinallvariablesamongstpatientsregardingrelapsestageandremissionstage(P>0.05).Conclusion:Therewasacellularimmunedeficiencyinpatientswithrecurrentgenitalherpes.
简介:ObjectivesTocomparetheefficiencyoffourcommercialELISAkitsindetectingtype-specificHSV-2IgGantibodies.MaterialsandMethods:Atotalof125subjects,including105withgenitalulcers,and20controlswithoutanyhistoryofSTDswererecruitedfromtheSTDclinicfordetectionoftype-specificHSV-2IgGantibodywithdifferentkits.FourkindsofcommerciallyavailableELISAkits,includingQuidaHSV2IgGELISA(AifulangBiochemCo.Ltd.,Hangzhou),TORCH-HSV2IgG(JingmeiBiotechCo.Ltd.,Shanghai),Captia^TMHSV2IgG(Trinitybiotech,USA)andHerpeSelectT^TM2ELISAlgG(Focustechnologies,USA)wereusedforevaluation.WesternBlotassaywasperformedasagoldstandard.Results:ComparedtoWesternBlotresults,thesensitivityandspecificityofthekits(QuidaHSV2,TORCHHSV2,Captia^TMHSV2andHerpeSelect^TM2)were13.1%and98.4%,7.5%and100%,100%and11.1%,87.7%and96.7%,respectively.Thepositivepredictivevalue(PV)andnegativePVofthefourkitswere88.9%and54.3%,100%and55.5%,55.6%and100%,96.2%and89.2%,respectively.TheareasundertheROCcurveofthreekits(QuidaHSV2,Captia^TMHSV2andHerpeSelect^TM2)were0.885(0.822-0.948),0.825(0.747-0.902),0.974(0.950--0.998),respectivelyConclusion:TheperformanceofHerpeSelect^TM2isthebestamongthefourkits.Theresultsalsoindicatethatthecommerciallyavailablekitsfordetectionoftype-specificHSV2antibodyshouldbere-evaluatedintermsoftheirvalidity.
简介:Objective:Toinvestigatetherolesofinterleukin-2(IL-2)andinterleukin-10(IL-10)inpathogenesisofearlysyphilis.Methods:TheserumlevelsofIL-2andIL-10in48patientswithearlysyphilisweredetectedbyABC-ELISA.Results:(1)ThelevelofIL-2inthepatientswithearlysyphiliswassignificantlyhigherthanthatinhealthycontrols,whilethatofIL-10waslower(P<0.001andP<0.001).(2)ThelevelsofIL-2andIL-10werealmostidenticalinpatientswithprimaryandsecondarysyphilis(P>0.05),aswellasbetweendif-ferentRPRtiters(P>0.05).(3)Aftertherapy,thelevelofIL-2decreasedmarkedly(P<0.05),whilethatofIL-10increase(p>0.05).(4)AsignificantcorrelationwasfoundbetweentheserumlevelsofIL-2andIL-10(r=0.5385P<0.05).Conclusions:Th1up-regulationoccursinpatientswithearlysyphilis,andplaysanactiveroleinfightingagainstTPinfection.