简介:摘要目的探讨糖链抗原19-9(CA19-9)、CA72-4和癌胚抗原(CEA)与胃蛋白酶原(PG)Ⅰ和Ⅱ联合检测在胃癌诊断中的临床应用价值。方法连续收集北京积水潭医院消化科收治的50例胃癌(GC)和50例胃良性病变(NGD)患者的血清标本。应用胃癌生物标志物抗体芯片定量检测患者血清中CA19-9、CA72-4、CEA、PGⅠ和PGⅡ的浓度。计算其单独及联合检测用于胃癌诊断的效能。结果上述五项指标在GC组中的浓度均显著高于NGD组(P<0.05)。其单独检测用于胃癌诊断的ROC曲线下面积(AUC)在0.586(95%CI:0.471~0.701)至0.811(95%CI:0.719~0.902)范围内波动,即CA72-4最低,PGⅡ最高。CA19-9、CA72-4和CEA三者联合检测的AUC略低于PGⅠ和PGⅡ两者联合检测,分别为0.797(95%CI:0.710~0.885)和0.814(95%CI:0.724~0.905),而上述所有指标联合检测的AUC最高,可进一步升至0.875(95%CI:0.800~0.943)。结论应用抗体芯片法联合检测传统血清学肿瘤标志物和胃蛋白酶原对辅助胃癌诊断具有潜在价值,值得进一步推广和应用。
简介:AIM:ToexploreexpressionsofPIK3CAintheprogressionofgastriccancerfromprimarytometastasisanditseffectsonactivationofphosphatidylinositol3-kinase(PI3K)/Aktpathway.METHODS:mRNAandproteinlevelsofPIK3CAwereassessed,respectively,byreal-timequantitativepolymerasechainreactionandimmunohistochemistryinspecimensofnormalgastricmucosa,primaryfociandlymphnodeanddistantmetastasisofgastriccancer.AktandphosphorylatedAktproteinwerealsoexaminedbyWesternblottinginthesetissues,inordertoanalyzetheeffectofPIK3CAexpressionlevelchangesontheactivationofPI3K/Aktsignalingpathway.RESULTS:PIK3CAmRNAinlymphnodemetastasiswereapproximately5and2foldshigher,respectively,thanthatinthecorrespondingnormalgastricmucosaandprimarygastriccancertissues(P<0.05),whilenostatisticalsignificancewasfoundcomparedwithdistantmetastasis.Immunohistochemically,PIK3CAproteinexpressionwasdiscoveredin7(35%)specimensof20primaryfocivs10(67%)of15oflymphnodemetastasisor11(61%)of18ofdistantmetastasis(35%vs67%,P=0.015;35%vs61%,P=0.044).WiththeincreasedlevelofPIK3CAexpression,thetotalAktproteinexpressionremainedalmostunchanged,butp-Aktproteinwasupregulatedmarkedly.CONCLUSION:IncreasedexpressionofPIK3CAisexpectedtobeapromisingindicatorofmetastasisingastriccancer.Up-regulationofPIK3CAmaypromotethemetastasisofgastriccancerthroughaberrantactivationofPI3K/Aktsignaling.
简介:摘要在脓毒性休克患者早期诊治中,常规血流动力学参数及氧供参数并不能准确判断组织缺氧的存在,而与这些参数相比,静-动脉二氧化碳分压差/动-静脉氧含量差比值(Pv-aCO2/Ca-vO2)能够迅速地反映机体无氧代谢的状态,且与乳酸及乳酸清除率有较强的相关性。因此,在脓毒性休克患者早期复苏阶段,Pv-aCO2/Ca-vO2比值可以作为一个较好的生物标志物来评估液体反应性及预测不良预后。
简介:Kineticsofchloride/sulfate,chloride/nitrateandnitrate/sulfateforwardexchangesandreverseexchangesatsolutionconcentrationsof1N,0.2Nand0.02Non201×7strongbaseanionexchangeresin,respectively,havebeenexaminedat25℃.Itisfoundthattheforwardandthereverseexchangeratesofthetwogivenionsatlowsolutionconcentration,respectively,underidenticalconditionscanbecontrolledbydifferentmechanisms,whilethoseathighsolutionconcentrationareallcontrolledbyparticlediffusion.Theternaryexchangeratesofsulfate/(chloride+nitrate)andnitrate/(chloride+sulfate)havealsobeenexamined.